Preparing Gels - abi PRISM 377 User Manual

Preparing Gels

Pouring Gels
To pour gels for use on the ABI P
Step
2-2 Gels
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Action
1 Cast the gels as instructed in the ABI P
User's Manual. Use 0.2-mm spacers (P/N 401837) and a 0.4-mm
96-lane casting comb (P/N 4305385) with the new stepped front
plate (P/N 4305384).
! WARNING ! CHEMICAL HAZARD. Acrylamide and Bis-
Acrylamide are both poisons, neurotoxins, irritant,
carcinogens, and possible teratogens. Acrylamide and Bis-
Acrylamide sublimes (the solid releases toxic vapor) and is
harmful if swallowed, inhaled, or absorbed through the skin.
Effects are cumulative. When handling, always wear personal
protection (i.e., lab coat, safety glasses, and chemical
resistant gloves) and use in a well ventilated area. Thoroughly
clean surfaces subject to contamination (i.e., binder clips,
combs, and glass plates).
2 Clamp the gels as shown below. Use three stainless steel "bulldog"
binder clips (P/N 4305386) on the top.
IMPORTANT To prevent well leakage, the 96-lane plates and
casting combs require 10–12 lbs. clamping pressure.
End clamps align with
front plate notch
IMPORTANT Be careful not to damage the teeth of the comb
when attaching the clamps.
377 96-lane DNA sequencer:
RISM
377 DNA Sequencer
RISM
Clamping pressure
on casting comb