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Step
10
2-4 Gels
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Action
9
Carefully insert the comb into the gel.
a.
Carefully align the center registration line on the comb with the
registration mark on the back plate.
b.
Slide the comb between the plates.
IMPORTANT
To avoid bending or breaking the teeth of the
comb, ensure all teeth enter the space between the plates at the
same time. Do not force the comb into the gel because the teeth will
bend, causing leaking.
c.
Continue to slide the comb down until the tips of the teeth just
touch or slightly depress the surface of the gel.
d.
Teeth should just barely indent the surface of the gel. If the
surface of the gel is not completely flat in the loading region,
insert some of the teeth below the surface of the gel (up to
0.5 mm) so that all of the teeth touch the gel surface.
e.
If a tooth has penetrated the gel surface do not attempt to
withdraw the comb. This will cause sample to leak into adjacent
wells.
Place the gel and the cassette in the 377 instrument.
Note
For instructions on setting up the 377 instrument for a run
refer to the ABI P
RISM
377 DNA Sequencer User's Manual.
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Table of Contents
Troubleshooting
