abi PRISM 377 User Manual page 30

Thermistors
Problem
Error: "Thermistor
Failure"
Error: "Temperature
below thermistor limit."
Results
Problem
Odd and even
lanes overlap
Signal showing up
in neighboring
lanes
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
Possible Cause
One or more
thermistors are bad
Ambient temperature is
too low (< 21.9 °C) for
100k thermistor
Possible Cause Solution
Running too long Shorten the run time between
between loadings.
staggered
loadings
Too much salt in Resuspend samples in
the sample formamide only.
Perform extra 70% ethanol
rinse of samples if
precipitated (may lead to
slight loss in signal).
Leaky lanes Check clamps and comb fit.
Signal intensity Move tracker lane position
very high and from center of band to the
signal is being edge of the band away from
detected in the strong signal and extract
neighboring lanes as usual.
due to closeness
Use one or two lane
of spacing
averaging to extract lanes.
Load less volume.
Solution
Schedule a service call
to replace the
thermistors. Continue
to use the instrument
as usual.
Turn on the pump
to warm the
coolant to above
21.9 °C.
Scedule a service
call service to
replace the
thermistors.
Continue to use
the instrument as
usual.
Troubleshooting 4-3