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Suggested Loading
Follow the loading procedure to load the odd lanes first, electrophorese
for 2 min, then load the even lanes.
Sequence
IMPORTANT
table below.
If you are running...
Sequencing
GeneScan
Loading
To load the gel:
Procedure
Step
2-10 Gels
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If any wells leak, flush the contaminated lanes then follow the
Then...
Electrophorese immediately, then after each three
loads.
Leaking wells are not tolerated in GeneScan
applications. If a well leaks, it is best to run another
gel. At the very least, do not use the wells around
the leaking lane.
Action
1
Press PAUSE during the prerun.
2
Flush the wells with 1x TBE using a syringe.
Note
Use care when flushing the wells: Too much pressure
could tear the wells, and touching the teeth with the syringe could
damage the comb and displace the teeth, which could cause
leakage.
3
Using the two-pitch, eight-channel loader, draw 2 µL of sample into
the needles.
4
Clear any air gaps in the needles by dispensing 0.5 µL of sample,
or by dispensing until sample is visible in the tips of the syringe.
5
Using the comb markers as a guide, align the needles into their
respective lanes.
6
Very slowly dispense up to 1.5 µL of samples into the wells. Load
the odd lanes first.
Note
For longer reads, load the samples close to the gel surface
rather than from the top of the well. To accomplish this, the two-
position loading syringe must have needles with 0.2-mm or 0.25-
mm outer diameters. With 0.3-mm outer diameter needles, the
samples must be gravity loaded.
7
After each loading, rinse the needles with warm dH
with a lint-free tissue to remove residual salt and prevent clogging.
8
Continue to load until all odd lanes have been loaded.
O and blot dry
2
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Troubleshooting
