Page 1
Translation of the original operating manual 06/2020 Rev. 1.2 of 28/04/2022 Sigma 4-5KL;Refrigerated centrifuge;Sigma 4-5KL IVD; ; Refrigerated centrifuge part no. 0703602 Sigma 4-5KL Sigma 4-5KL IVD from serial no. 180163 Refrigerated centrifuge Operating Manual Please retain for later use!
Page 2
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 2 / 84 Translation of the original operating manual, part no. 0703602...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................
Page 6
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Table of contents Storage and transport ....................... 29 Dimensions and weight ....................29 Storage conditions ......................29 Notes on transport ......................29 Packaging ........................30 Transport safety device ....................30 Set-up and connection ...................... 31 Installation site ........................
Page 7
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Table of contents Malfunctions and error correction ..................51 General malfunctions ...................... 51 7.1.1 Emergency lid release ....................52 Table of error codes ......................53 Service contact ....................... 54 Maintenance and service ....................55 Maintenance ........................
Page 8
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Table of contents Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 8 / 84 Translation of the original operating manual, part no. 0703602...
• compliance with the inspection and maintenance instructions. Sigma Laborzentrifugen GmbH cannot be held liable for: • damage resulting from the improper use of the centrifuge not in line with its intended purpose, •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 1 General information → Copyright The copyright concerning the operating manual remains with Sigma Laborzentrifugen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that must not be •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 2 Layout and mode of operation → Layout and mode of operation → Layout of the centrifuge 2.1.1 Functional and operating elements User interface (see chapter 6.3.1 - "User interface") Mains switch Fig.
Page 12
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 2 Layout and mode of operation Circuit breaker Mains power input Abb. 3: Detailed view A - Mains power input Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 12 / 84...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 2 Layout and mode of operation 2.1.2 Name plate Manufacturer Power consumption Max. speed Max. kinetic energy Serial number Part number Type Nominal voltage Consult operating manual 10 Symbol for special disposal (see chapter 9 - "Disposal")
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 2 Layout and mode of operation → Mode of operation → 2.2.1 Centrifugation principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions, or gas mixtures) into their components.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 2 Layout and mode of operation → 2.2.2.1 Speed, radius, and relative centrifugal force The acceleration g, which the samples are subject to, can be increased by increasing the radius in the rotor chamber and by increasing the speed.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → Safety → Marking of the unit The following symbols are used on this centrifuge: Arrow indicating the On (Power) direction of rotation Rotor loading information Off (Power) (see chapter 2.1.2 - "Name plate")
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → Explanation of the symbols and notes In this operating manual, the following names and symbols to indicate hazards are used: This symbol stands for a direct hazard to the life and health of persons.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → Responsibility of the operator The operator undertakes to authorise only trained, specialised personnel to work on the centrifuge (see chapter 3.4 - "Requirements concerning the personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → Requirements concerning the personnel Risk of injury if the personnel are not sufficiently qualified If unqualified personnel perform work on the centrifuge or are present in the danger zone of the centrifuge, hazards result that can cause serious injuries and considerable damage to property.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety The operating personnel must ensure that any serious event which either directly or indirectly had, could have had or could have one of the following consequences will be reported to the manufacturer or competent authority:...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → Safety instructions 3.6.1 Electrical safety As protection against electric shock, the centrifuge is equipped with an earthed mains power cable and connector. To ensure the effectiveness of this safety feature, the following must be ensured: •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety • Do not spin any substances that could damage the material of the rotors and buckets of the centrifuge in any way. Highly corrosive substances, for example, damage the material and affect the mechanical strength of the rotors and buckets.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and connection").
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety 3.6.7 Service life (for centrifuges according to Machinery Directive) The rotors and accessories have a limited service life. • Perform regular checks (at least once per month) for safety reasons! •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety 3.6.8 Service life (for centrifuges according to the IVD regulation) Centrifuge The service life of the centrifuge depends on several factors, e.g. the type and frequency of use, the area of application and the performance of service and maintenance tasks.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety 3.6.9 Safety of rotors and accessories 3.6.9.1 Marking During production, every rotor and bucket receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection. Some rotors also have an additional serial number providing further detailed information.
(see chapter 7.2 - "Table of error codes"). 3.7.4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument. Please contact the Sigma service department (see chapter 7.3 - "Service contact"). → 3.7.5 Imbalance monitoring system The indication "Imbalance"...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 3 Safety → Measures in the event of hazards and accidents • If an emergency arises, switch off the centrifuge immediately! • If in doubt, call the emergency doctor! DANGER →...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 4 Storage and transport → Storage and transport → Dimensions and weight 4-5KL, 4-5KL IVD Height: 390 mm Height with open lid: 883 mm Width: 605 mm Depth: 678 mm Weight: 110 kg →...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 4 Storage and transport Packaging The centrifuge is packaged in a wooden crate. • After taking off the lid, remove the side panels. • Remove the packaging material. • Lift the centrifuge upwards with a lifting device to lift it safely. When lifting the centrifuge, always reach under the centrifuge from the side.
CAUTION Sigma centrifuges are units of protection class I. The centrifuges of this model series have a three-wire power cord with an IEC C13 connector. The removable power cord must not be longer than 3 m!
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 5 Set-up and connection The centrifuges are equipped with a mains power switch with an integrated thermal circuit breaker. • Switch the unit off by actuating the mains power switch. •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → Using the centrifuge → Initial start-up • Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 - "Set-up and connection").
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → 6.2.2 Installation of rotors and accessories → 6.2.2.1 Installation of a standard rotor • Open the centrifuge lid by pressing the lid key. • Unscrew the rotor tie-down screw from the motor shaft (counter- clockwise).
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge 6.2.2.2 Installation of accessories • Only use inserts that are suitable for the rotor. • All buckets of the swing-out rotor need to be installed when spinning.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge Pay attention to the marking of the centrifuge (see the illustration below)! Safety indications on the centrifuge must be kept readable at all times. If necessary, they must be replaced.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → Control system "Spincontrol L" → 6.3.1 User interface The centrifuge is operated via three buttons with integrated light-emitting diodes and one function knob. The display is divided into several different fields.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → 6.3.2 Manual mode → 6.3.2.1 Starting a centrifugation run The centrifuge is ready for operation when the start key is illuminated. • Press the start key in order to start a centrifugation run.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → 6.3.2.5 Speed / relative centrifugal force (RCF) The set speed of the centrifuge is displayed in the upper area of the Speed/RCF field. The actual value is displayed right below. The speed is...
Page 40
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge Runtime as of the set speed If the runtime is to be counted as of the moment when the set speed is reached, the symbol (see the illustration) behind the set speed value must be activated: •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge Short run A short run can be started if no run is active. • Keep the start key pressed during the short run. During the short run, the centrifuge accelerates with the acceleration curve 9 (maximum) until the maximum permissible speed of the rotor is reached.
Page 42
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge Program "Rapid Temp" The centrifuge has a special "Rapid Temp" program that precools the centrifuge rapidly under defined conditions. • Select the program with the aid of the menu item "run Prog" (see chapter 6.3.3.2 - "Loading a program").
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → → 6.3.2.8 Rotor selection This field shows the rotor that is currently being used. The rotor selection can only be changed when the centrifuge is at a standstill.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → 6.3.2.9 Acceleration and deceleration curves Acceleration This function is used to select an acceleration curve. The system offers 10 fixed, programmed acceleration curves (curves 0-9). Brake This function is used in order to select a curve that decelerates the centrifuge to a standstill.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → 6.3.2.11 Sound signal ("Buzzer") This function is used to set an acoustic warning signal that sounds at the end of the centrifugation run and also in the event of an imbalance or error message.
WARNING The cycle display will be reset after the rotor and buckets have been replaced by the service department of Sigma Laborzentrifugen GmbH (see chapter 7.3 - "Service contact"). Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 46 / 84 Translation of the original operating manual, part no.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge 6.3.2.14 Input lock In order to prevent the centrifuge from being manipulated by unauthorised persons, inputs can be disabled via the menu. Inputs via the keypad, i.e. for starting or stopping a centrifugation run or for opening the lid, are enabled.
Page 48
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge After the end of the turn-off countdown, the refrigeration unit will be switched off and the centrifuge lid will be opened so that any remaining moisture can dry. The display continues to show "Eco".
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge → 6.3.3 Program mode A program contains all the data that are required for a centrifuge run. Certain sedimentation results can be repeated under identical conditions. Programs can be saved, loaded, executed, and edited when the centrifuge is at a standstill.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 6 Using the centrifuge 6.3.3.2 Loading a program • Select the menu item "run Prog" and confirm the selection. The indication "run" flashes in the activated status. • Select the desired program and confirm the selection by pressing the function knob.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 7 Malfunctions and error correction → Malfunctions and error correction → General malfunctions Error messages are displayed as "Error" followed by a code number. If the acoustic signal is activated, it sounds when the error message is displayed.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 7 Malfunctions and error correction 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 7 Malfunctions and error correction → Table of error codes Error no. Kind of error Measures Note • System error Allow to slow down All these errors stop the centrifuge or cause it to •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 7 Malfunctions and error correction → Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → 8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration system) In order to cool the refrigerant that is compressed by the refrigeration unit, centrifuges with an air-cooled refrigeration system use a lamellar condenser.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → 8.1.2.1 Plastic accessories The chemical resistance of plastic decreases with rising temperatures (see chapter 11.5 - "Resistance data"). • If solvents, acids, or alkaline solutions have been used, clean the plastic accessories thoroughly.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → 8.1.4 Load bearing bolts Only greased load-bearing bolts ensure a uniform swing-out of the buckets and, therefore, the smooth operation of the centrifuge. Load-bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → 8.1.5 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g. with a vacuum cleaner). Replace the rubber cushions since even thorough cleaning will not remove all glass particles.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. • Only qualified specialist personnel is authorised to perform this service work.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 8 Maintenance and service → Return of defective centrifuges or parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer.
Page 63
NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 63 / 84 Translation of the original operating manual, part no. 0703602...
Disposal → Disposal of the centrifuge In accordance with the directive 2012/19/EU, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste. •...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 10 Technical data → 10.1 Ambient conditions • The figures are valid for an ambient temperature of +23°C and a nominal voltage 10 %. The minimum temperature is ≤ +4°C and depends on the rotor type, speed, and ambient temperature.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix → 11 Appendix → 11.1 Range of accessories The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. 11.1.1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix → 11.3 Acceleration and deceleration curves Linear curves are numbered in the direction of increasing acceleration (from right to left). The deceleration curves are inverted images of the acceleration curves and are assigned the same numbers.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix → 11.4 Table of the service life of rotors and accessories • The rotors and accessories must be put out of service after 10 years. Any use after this period may be permissible in individual cases after an inspection performed by the manufacturer.
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix → 11.5 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde...
Page 72
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate...
Page 73
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate...
Page 74
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix Declaration of conformity – China RoHS 2 11.7 Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 79 / 84 Translation of the original operating manual, part no. 0703602...
Page 80
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD 11 Appendix Version 06/2020, Rev. 1.2 of 28/04/2022 • sb 80 / 84 Translation of the original operating manual, part no. 0703602...
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Index 12 Index Changes in structure ........60 Chemical and biological safety ....22 Acceleration ..........44 Chemical resistance of plastic ....57 Acceleration curves ......44, 69 China RoHS 2 – Declaration of conformity . 79 Accessories, cleaning and care ....
Page 82
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Index Dimensions ..........29 Imbalance error .......... 53 Direct hazard to the life and health ..... 17 Imbalance monitoring system ....27 Directive 2002/96/EC ......... 64 Importance of the operating manual ..... 9 Disinfectants ..........
Page 83
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Index Maximum speed for tubes ......36 Measures in the event of hazards and Qualified electrician ........19 accidents ..........28 Quick stop ..........38 Mechanical safety ........21 Quitting the modification mode ....38 Mode of operation ........
Page 84
Refrigerated centrifuge Sigma 4-5KL Refrigerated centrifuge Sigma 4-5KL IVD Index Saving a program........49 Switching the centrifuge on ......33 Scope of supply ......... 10 System check ..........27 Selection, display, and modification of data 38 System error ........51, 53 Serial number ........
Need help?
Do you have a question about the 4-5KL and is the answer not in the manual?
Questions and answers