Sigma 4-15C Operating Manual
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Sigma 4-15C Operating Manual

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Centrifuge
4-15C
Operating Manual

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  • Page 1 — — — — — — — — — — — — — — — Centrifuge 4-15C Operating Manual...
  • Page 3 Dear customer, Congratulations for purchasing a SIGMA laboratory centrifuge. You have selected a device which combines many advantages. A wide spectrum of programming options and an electronic operation control allow a trouble- free use of the centrifuge. With its 3-phase drive, maintenance-free, quiet operation without any carbon dust pollution is guaranteed.
  • Page 5 Sigma Laborzentrifugen An der Unteren Sö se 50 D-37520 Osterode 01.02.2002 Geschä ftsführer Managing Director Directeur Gé rant ................ Fabr. Nr. Serial No. Numé ro de fabrique Konformitätserklärung dreisprachig 4-15 20020201.DOC Operating Manual SIGMA 4-15C, page 5 of 95 03/06...

  • Page 7: General Information

    Fuses / emergency circuit breaker on site Installation of rotors and accessories 3.3.1 Fastening of angle rotors with hermetically sealed lid Initial start-up 3.4.1 Switching on of the centrifuge 3.4.2 Opening lid 3.4.3 Installation of a rotor Operating Manual SIGMA 4-15C, page 7 of 95 03/06...

  • Page 8: Operating Elements

    Alteration of existing curves 5.1.5 Alteration of the contrast 5.1.6 Imbalance monitoring 5.1.7 Shortrun and faststop Programming Load, save and delete programs 6.1.1 Load a program 6.1.2 Save a program 6.1.3 Delete a program Operating Manual SIGMA 4-15C, page 8 of 95 03/06...

  • Page 9: Care And Maintenance

    9.5.2 Centrifuge decelerates during operation 9.5.3 Lid cannot be opened/closed 9.5.4 Emergency lid release 9.5.5 Problems with the centrifuge? Error codes Speed-RCF-diagram Declaration of decontamination/Return declaration Form program data 9.10 Leaflet Operating Manual SIGMA 4-15C, page 9 of 95 03/06...

  • Page 11: Technical Data

    The figures are valid for an ambient temperature of 23 °C +/- 2 °C and nominal voltage +/- 5 %. (Allowable ambient temperature +4 °C - +40 °C; max. humidity 80 %.) Subject to technical alterations. Operating Manual SIGMA 4-15C, page 11 of 95 03/06...
  • Page 13 7.8 cm 4 300 3 680 13235 Bucket, aluminium, for 7 culture tubes 50 ml 15151, suitable for 11150, max. radius 19.0 cm, min. radius 9.3 cm 4 500 4 302 Operating Manual SIGMA 4-15C, page 13 of 95 03/06...
  • Page 14 10-12 ml, max. ∅ 16.2/19 x 65 x 90 mm, e.g. 13026 plus 17126, 15000, 15010, 15039, polypropylene 17356 Round carrier for 16 Vacutainer/hemolyse/ RIA-tubes 5-6 ml, max. ∅ 13.5/17.5 x 70 - 90 mm, polypropylene Operating Manual SIGMA 4-15C, page 14 of 95 03/06...
  • Page 15 Round carrier for 4 culture tubes 50 ml with skirt, polypropylene 17385 Round carrier for 1 tube with screw cap 78-85 ml, max. ∅ 38/40 x 85 - 115 mm, e.g. 13085 plus 17185, 15074, 15075, 15076, 15080, polypropylene Operating Manual SIGMA 4-15C, page 15 of 95 03/06...
  • Page 16 4 120 data with 13127: max. radius 19.1 cm, min. radius 9.7 cm 4 500 4 324 11140 Swing-out rotor for 4 buckets for the following accessories 5 000 4 668/4 416 Operating Manual SIGMA 4-15C, page 16 of 95 03/06...
  • Page 17 ∅ 13.5/18 x 65 - 90 mm, polypropylene 18015 Rectangular carrier for 12 tubes 10-15 ml, max. ∅ 17 x 90 - 105 mm, e.g. 15015, 15020, 15022, 15023, 15024, polyallomer Operating Manual SIGMA 4-15C, page 17 of 95 03/06...
  • Page 18 118 mm, polypropylene 18085 Rectangular carrier for 1 tube with screw cap 78-85 ml, max. ∅ 38/40 x 85 - 112 mm, e.g. 13085 plus 17185, 15074, 15075, 15076, 15080, polyallomer Operating Manual SIGMA 4-15C, page 18 of 95 03/06...
  • Page 19 Swing-out rotor 72 x 15 ml complete, consisting of rotor 11156, 6 rectangular buckets 13115, 6 carriers 18015 and 72 polystyrene tubes 15020, max. radius 18.2 cm, min. radius 9.5 cm 4 500 4 120 Operating Manual SIGMA 4-15C, page 19 of 95 03/06...
  • Page 20 12130 Angle rotor 30 x 1.5-2.2 ml for reaction vials, e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius 10.0 cm, min. radius 6.7 cm, angle 45° 13 500 20 376 Operating Manual SIGMA 4-15C, page 20 of 95 03/06...
  • Page 21 15005, ∅ 7.9/10 x 28/31 mm suitable for 11148, 12130, 12200, 17353, 18002, 18003 Adapter for PCR-tube 0.2 ml, Ø 5.85/6.95 x 13021 20/23.4 mm, suitable for 11148, 12130, 12200, 17353, 18002, 18003 Operating Manual SIGMA 4-15C, page 21 of 95 03/06...
  • Page 22 15060 75 mm, suitable for 13233, 17352, 17356, 18005, 18009, 18105 Stainless steel tube 10 ml, ∅ 15.7 x 76 mm, 13026 closeable with cap 17126, suitable for 12168, 17355, 18010 Operating Manual SIGMA 4-15C, page 22 of 95 03/06...
  • Page 23 17375, 18050 13055 Stainless steel tube 50 ml, sealable with cap 17054, ∅ 29 x 101.5 mm, suitable for 12166, 13082, 17376, 18051 17054 Stainless steel sealing cap for 13055 Operating Manual SIGMA 4-15C, page 23 of 95 03/06...
  • Page 24 13234. 17390, 18100 15103 ditto, polycarbonate, graduated 2 - 100 ml in steps of 2 ml 15125 Polypropylene bottle with screw cap 125 ml, ∅ 51 x 99 mm, suitable for 17395, 18125 Operating Manual SIGMA 4-15C, page 24 of 95 03/06...
  • Page 25 0 - 5.5 ml in steps of 0.1 ml Glass tube 10 - 12 ml, ∅ 16 x 100 mm, 15015 suitable for 17358, 18015, 18115 15024 ditto, graduated 0 - 10 ml in steps of 0.1 ml Operating Manual SIGMA 4-15C, page 25 of 95 03/06...
  • Page 26 Rubber adapter for 15 ml glass tube 15022, suitable for 12166 16019 Rubber adapter for 15 ml glass tube 15022, suitable for 12165 16030 Rubber adapter for 30 ml glass tube 15033, suitable for 12166 Operating Manual SIGMA 4-15C, page 26 of 95 03/06...

  • Page 27: Maximum Speed For Tubes

    We recommend to always fill up the tubes and to follow the recommendations of the manufacturer. Operating Manual SIGMA 4-15C, page 27 of 95 03/06...
  • Page 28 Operating Manual SIGMA 4-15C, page 28 of 95 03/06...

  • Page 29: Scope Of Supply

    Rotor Part No. Rotor No..............................................................Standards and Regulations Please refer to the enclosed EU-Statement of Conformity. Operating Manual SIGMA 4-15C, page 29 of 95 03/06...

  • Page 30: Safety Instructions

    Keep your hand away from the danger zone when closing the centrifuge lid. Risk of bruising! 10.Attention! Defective lid relieving devices could cause the centrifuge lid to fall down (contact Service). Risk of bruising! Operating Manual SIGMA 4-15C, page 30 of 95 03/06...

  • Page 31: Symbol Table

    Tirer la fiche de prise Vorsicht Quetschgefahr Caution! Risk of bruising Attention! Danger de blessure Drehrichtungspfeil → Arrow direction of rotation Flèche sens de rotation Heiße Oberflä che Hot surface Surface chaude Operating Manual SIGMA 4-15C, page 31 of 95 03/06...

  • Page 32: Construction And Constructive Safety Measures

    General Outlay The new generation of SIGMA laboratory centrifuges is equipped with two microprocessors which guarantee independently the control of the rotor recognition and the overspeed signal. A further optimization and increase of the instrument’ s safety could be maintained. The long-life asynchronous motor is silent and brushless.

  • Page 33: Electronic Control

    (refer to chapter 9.5.4 "Emergency lid release"). 2.6.2 Imbalance Monitoring System In the event that uneven loading leads to imbalance, the drive is switched off and an imbalance warning message is displayed. Operating Manual SIGMA 4-15C, page 33 of 95 03/06...

  • Page 34: Standstill Monitoring

    Ground Wire Check For ground wire check there is a ground screw at the rear panel of the centrifuge. A ground wire check can be carried out using an appropriate measuring instrument. Operating Manual SIGMA 4-15C, page 34 of 95 03/06...

  • Page 35: Unpacking Of The Centrifuge

    Please keep case for possible transport of centrifuge later. 3.1.1 Transport Safety Device The SIGMA 4-15C has a transport safety device which must be removed before start-up. The transport safety device blocks the drive system. The transport safety device screws are accessible from the outer underside.

  • Page 36: Installation

    The operating voltage on the name plate must correspond to the local supply voltage! SIGMA laboratory centrifuges are units of safety class I, DIN VDE 0700, and in- clude a three wire power cord 2,5 m long with shockproof right angle plug. The instrument has thermal fuses.

  • Page 37: Installation Of Rotors And Accessories

    11. In angle rotors the plastic vessels must always be totally filled to avoid cracks of vessels and leakages or loosening of the caps in case of partial filling. Attention, follow the special comments of chapter 1.5. Operating Manual SIGMA 4-15C, page 37 of 95 03/06...
  • Page 38 To increase life of rotors and seals the rotors must be cleaned with slushing oil and the seals and thread areas with vaseline or grease after cleaning. Attention! Please follow the special comments of chapter 1.5. Operating Manual SIGMA 4-15C, page 38 of 95 03/06...
  • Page 39 Operating Manual SIGMA 4-15C, page 39 of 95 03/06...

  • Page 40: Initial Start-Up

    3.3 "Installation of rotor and accessories"). Please pay attention to the fact that during tightening the disc spring of the rotor tie-down screw is pressed together and the screw is tightened. Operating Manual SIGMA 4-15C, page 40 of 95 03/06...

  • Page 41: Operating Panel

    Operating Panel Operating panel The centrifuge can be operated via the operating panel. Keys can be pressed when their LED is on. Operating Manual SIGMA 4-15C, page 41 of 95 03/06...
  • Page 42 This key is used to open the lid. This can only be executed if • the centrifuge has come to a complete stop • the Lid-key is illuminated. 4.1.4 Knob This knob can be used for selecting and alterating parameters and figures/ numbers. Operating Manual SIGMA 4-15C, page 42 of 95 03/06...

  • Page 43: Relative Centrifugal Force (Rcf)

    The set value of this parameter is in the upper section of this area, underneath is the actual value. The maximal RCF-values are rotor dependent. (Refer to chapter 9.4 "Mathematical relations") Operating Manual SIGMA 4-15C, page 43 of 95 03/06...
  • Page 44 By entering a specific run time the continuous mode is deactivated. It can be terminated by pressing the Stop-key as well. Continuous run Operating Manual SIGMA 4-15C, page 44 of 95 03/06...

  • Page 45: Program (Prog)

    Storage space for fifty programs - No. 1 – 50 - from which you can select and load a program is at your disposal. The actual loaded program is indicated by "--". Operating Manual SIGMA 4-15C, page 45 of 95 03/06...
  • Page 46 Parameters (PARA) Parameters If this area has been selected, parameters can be changed and the start delay and the automatic lid opening after end of run can be activated or deactivated. Operating Manual SIGMA 4-15C, page 46 of 95 03/06...
  • Page 47 Curve no. 0 represents brakeless deceleration. 4.2.7.3 Radius Radius The set radius will determine the displayed RCF-value. If the radius is not changed, the max. RCF-value will be displayed. Operating Manual SIGMA 4-15C, page 47 of 95 03/06...

  • Page 48: Start Delay

    With this function a start delay can be set. 4.2.7.6 Automatic Lid Opening after End of Run If this parameter has been set the lid will automatically open after standstill of the rotor. Operating Manual SIGMA 4-15C, page 48 of 95 03/06...
  • Page 49 Selection of configuration (CONFIG) opens a menu where Code, Language, Screen, Fine, Buzzer, Sensor, Info, Reset and the creation of freely programmable curves can be chosen. Furthermore, the cycles and run times of the individual rotors can be read. Operating Manual SIGMA 4-15C, page 49 of 95 03/06...
  • Page 50 Then, the centrifuge starts with the selected values. Notice: All entry limits are automatically checked. When reaching a limit, the counting operation is stopped (refer to chapter 9.3 "Entry limitations"). Operating Manual SIGMA 4-15C, page 50 of 95 03/06...
  • Page 51 • Confirm the selected parameter by pressing the knob (here: acceleration). The parameter is activated. Select the desired acceleration curve by turning the knob. Deceleration, radius and density are changed in the same way. Operating Manual SIGMA 4-15C, page 51 of 95 03/06...
  • Page 52 • Change the set time by turning the knob and deactivate the start delay by pressing the knob. The automatic lid opening after end of run is activated or deactivated in the same way. Operating Manual SIGMA 4-15C, page 52 of 95 03/06...
  • Page 53 Attention: Please take care that the correct rotor/bucket combination is entered as otherwise the max. allowable speed could be exceeded. This is not allowed. Operating Manual SIGMA 4-15C, page 53 of 95 03/06...
  • Page 54 5.1 "Selection, Display and Alteration of Program Parameters"). You cannot change: • Rotor • Program • Radius • Density The following functions can be activated/deactivated: • Start delay • Automatic lid opening after end of run Operating Manual SIGMA 4-15C, page 54 of 95 03/06...
  • Page 55 The sensor menu displays different signals. In case of a failure of the instrument this makes the diagnosis of errors and their fast repair through the service easier. Values can neither be entered nor altered. Operating Manual SIGMA 4-15C, page 55 of 95 03/06...
  • Page 56 - RCF magnified -Zoom- (no speed display) • Select the configuration mode by turning the knob. • After pressing the knob the configuration menu appears. • Select "SCREEN" by turning the knob. Operating Manual SIGMA 4-15C, page 56 of 95 03/06...
  • Page 57 • Select "SPEED - (ZOOM)" by turning the knob. • Activate the Speed-Zoom-screen by pressing the knob. • Select "EXIT" by turning the knob. Confirm it by pressing the knob. You get into the configuration menu again. Operating Manual SIGMA 4-15C, page 57 of 95 03/06...
  • Page 58 • By pressing the knob you return to the main display. The areas "SPEED" and "TIME" are displayed magnified. The area "RCF" disappears. Operating Manual SIGMA 4-15C, page 58 of 95 03/06...
  • Page 59 The max. preset final speed of one interval corresponds to the set speed of the run. The total time corresponds to the set time of the run. The final speed of the last interval is 0. Operating Manual SIGMA 4-15C, page 59 of 95 03/06...
  • Page 60 Select the configuration mode by turning the knob. • After pressing the knob the configuration menu appears. • Select "CURVES" by turning the knob. • After pressing the knob the curve menue appears. Operating Manual SIGMA 4-15C, page 60 of 95 03/06...
  • Page 61 Activate “0:00:00” by pressing the knob. • Set the time by turning the knob. • Save the time value in interval no. 1 by pressing the knob. • Select “SPEED” by turning the knob. Operating Manual SIGMA 4-15C, page 61 of 95 03/06...
  • Page 62 After pressing and turning the knob the time value of interval no. 2 appears. • Activate “0:00:00” of the second interval by pressing the knob. • Set the time by turning the knob. The extended curve appears. Operating Manual SIGMA 4-15C, page 62 of 95 03/06...
  • Page 63 Activate the speed value “0” by pressing the knob. • Change the speed value by turning the knob. • After pressing and turning the knob the time value of interval no. 3 appears. Operating Manual SIGMA 4-15C, page 63 of 95 03/06...
  • Page 64 Activate “0:00:00” of the third interval by pressing the knob. • Set the time by turning the knob. The extended curve appears. • Save the time value no. 3 by pressing the knob. Operating Manual SIGMA 4-15C, page 64 of 95 03/06...
  • Page 65 Select “EXIT” by pressing and turning the knob and leave the curve mode by pressing the knob again. • The created curve no. 20 is saved and can be recalled when programming the parameters. Operating Manual SIGMA 4-15C, page 65 of 95 03/06...
  • Page 66 Improper loading or malfunction during operation (e.g. glass breakage) resulting in an uneven run. Note! Additional information and a detailed description of errors and their correction is given in chapter 9.5 “Error correction“. Operating Manual SIGMA 4-15C, page 66 of 95 03/06...
  • Page 67 • Faststop Pressing the Stop-key during operation for longer than a second leads to maximum deceleration until standstill. Operating Manual SIGMA 4-15C, page 67 of 95 03/06...
  • Page 68 Activated program area • By turning the knob, all programs saved and the actual program ("--") appear one after the other. Changed program • Load desired program by pressing the knob. Operating Manual SIGMA 4-15C, page 68 of 95 03/06...
  • Page 69 • Select the program you want to load by turning the knob. After pressing the knob a dialogue window appears. Select the instruction "LOAD" and confirm it by pressing the knob. Dialogue window Operating Manual SIGMA 4-15C, page 69 of 95 03/06...
  • Page 70 • Select the desired program position in the list by turning the knob. • After pressing the knob a dialogue window appears. Select "SAVE" and confirm it by pressing the knob. Dialogue window Operating Manual SIGMA 4-15C, page 70 of 95 03/06...

  • Page 71: Delete A Program

    • Select the program you want to delete by turning the knob. • A dialogue window appears after pressing the knob. Select "DELETE" and confirm it by pressing the knob. Dialogue window Operating Manual SIGMA 4-15C, page 71 of 95 03/06...
  • Page 72 12. Fill vessels carefully to same weight. Imbalances would result in increased wear of bearings. 13. Grease joints of buckets and rotor pins in swing-out rotors. 14. Use perfect accessories only. 15. Avoid corrosion to accessories by careful maintenance. Operating Manual SIGMA 4-15C, page 72 of 95 03/06...
  • Page 73 18. Record all program data, refer to forms of appendix chapter 9.8. 19. When centrifuging substances with a density > 1,2 g/cm 3 the allowable max. speed must be reduced (refer to chapter 9.4.2 "Density"). Operating Manual SIGMA 4-15C, page 73 of 95 03/06...
  • Page 74 14. Lifting or moving of the centrifuge during operation. Leaning against or resting on the centrifuge is not allowed. 15. Do not place potential dangerous material - eg. glass vessels containing liquids - near the centrifuge. Operating Manual SIGMA 4-15C, page 74 of 95 03/06...
  • Page 75 19. Substances which could damage the material of the centrifuge, the rotors or the buckets must not be centrifuged. Infectious, toxic, pathogene or radioactive substances must be centrifuged in certified rotors and vessels only and all necessary safety precautions are taken. Operating Manual SIGMA 4-15C, page 75 of 95 03/06...
  • Page 76 Increased wear of the motor bearings will thus be avoided. If there is the risk of toxic, radioactive or pathogene contamination, special safety measures must be kept. Operating Manual SIGMA 4-15C, page 76 of 95 03/06...
  • Page 77 In order to avoid corrosion, rotor incl. tie-down screw and cover seal, buckets and carriers must be cleaned and greased regularly with the supplied slushing oil (SIGMA part no.: 70104 for 20 ml slushing oil). Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge.

  • Page 78: Glass Breakage

    Principally we want to point out that for centrifuging of e.g. infectious material certified and hermetically sealed accessories have to be used in order to avoid that the centrifuge is contaminated. Operating Manual SIGMA 4-15C, page 78 of 95 03/06...
  • Page 79 Rubber cushions 115-118 Round carriers for 13104/ 115-118 13117, Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Round carriers for 13350/ 115-118 13550, Polypropylene Rectangular carriers, 115-118 Polypropylene ditto, Polyallomer and 115-118 Polycarbonate Operating Manual SIGMA 4-15C, page 79 of 95 03/06...
  • Page 80 50.000 cycles. Any changes like e.g. corrosion, cracks, material abrasion etc. require special attention. 5. Screw connections have to be tight. Furthermore, the earth wire must be checked regularly. Operating Manual SIGMA 4-15C, page 80 of 95 03/06...
  • Page 81 (exception: curve 0). − Curve 0 decelerates brakeless. Comment: The acceleration time depends on the moment of inertia of the rotor. Linear Curves (0 - 9) Figure 2 Operating Manual SIGMA 4-15C, page 81 of 95 03/06...
  • Page 82 Slope 240 sec./1000 rpm. 180 sec./1000 rpm. 120 sec./1000 rpm. 60 sec./1000 rpm. 40 sec./1000 rpm. 30 sec./1000 rpm. 20 sec./1000 rpm. 10 sec./1000 rpm. 5 sec./1000 rpm. 0,9 sec./1000 rpm. Operating Manual SIGMA 4-15C, page 82 of 95 03/06...
  • Page 83 Quadratic curve no. Time up to 1000 rpm Slope from 1000 rpm linear sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. sec./1000 rpm. Operating Manual SIGMA 4-15C, page 83 of 95 03/06...
  • Page 84 If the density of the liquid is higher than 1.2 g/cm , the allowed maximum speed of the centrifuge is calculated according to the following formula: n = n , 1 ( Rho = density in g/cm Operating Manual SIGMA 4-15C, page 84 of 95 03/06...

  • Page 85: Error Correction

    • These error numbers indicate an error in to 77 after power on. the internal program storage. This error is e.g. generated when a power failure occurs during a storage procedure. Operating Manual SIGMA 4-15C, page 85 of 95 03/06...

  • Page 86: Emergency Lid Release

    The lid may only be unlocked and opened when the rotor is at standstill. 9.5.5 Problems with the Centrifuge? Please contact your supplier for support or in the event of malfunctions and for supply of spare parts. Operating Manual SIGMA 4-15C, page 86 of 95 03/06...

  • Page 87: Error Codes

    Fill in the forms and attach them when returning centrifuges, accessories and spare parts. Please understand that we cannot carry out any work before we have the declarations. (We recommend to make several copies of this page.) Operating Manual SIGMA 4-15C, page 87 of 95 03/06...
  • Page 89 Unit / component contaminated : Unit / component unused (new) : !!! Attention – This form must be glued on outside of the box !!! "---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 4-15C, page 89 of 95 03/06...
  • Page 91 I / we hereby declare that the information on this declaration are correct and complete. Company / Institute : Street : Postcode, City : Tel. : FAX : Name : Date : Stamp : Signature : "---------------------------------------------------------------------------------------------------------------------- Please make some copies before removing this page. Operating Manual SIGMA 4-15C, page 91 of 95 03/06...
  • Page 93 ........... Gravitational field ............. Rotor ............. Bucket ............. Made on: ..............................Operating Manual SIGMA 4-15C, page 93 of 95 03/06...
  • Page 95 Operating Manual SIGMA 4-15C, page 95 of 95 03/06...

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