Sigma 4-16KR Operating Manual
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Sigma 4-16KR Operating Manual

Refrigerated centrifuge for robot technology
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Operating Manual
Refrigerated Centrifuge
for Robot Technology
4-16KR
from serial no. 128012
Please retain for later use!
Version 10/2010, Rev. 1.9 of 02/07/2012
Translation of the original operating manual

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Summary of Contents for Sigma 4-16KR

  • Page 1 Operating Manual Refrigerated Centrifuge for Robot Technology 4-16KR from serial no. 128012 Please retain for later use! Version 10/2010, Rev. 1.9 of 02/07/2012 Translation of the original operating manual...
  • Page 3 4-16KR In case of inquiries please state the following number: Serial number: Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Phone +49 (0) 55 22 / 50 07-0 +49 (0) 55 22 / 50 07-12 E-mail: info@sigma-zentrifugen.de...

  • Page 5: Table Of Contents

    4-16KR Table of Contents Table of Contents General Information Importance of the Operating Manual ................9 Intended Use ......................9 Warranty and Liability ....................9 Copyright ........................10 Standards and Regulations ..................10 Scope of Supply ....................... 10 Design of the Centrifuge Overview ........................
  • Page 6 4-16KR Table of Contents Storage and Transport Dimensions and Weight .................... 21 Storage Conditions ....................21 Notes on Transport ....................21 Set-up and Connection Unpacking the Centrifuge ..................22 Transport Safety Device ................... 22 Installation Site ......................23 Power Supply ......................23 5.4.1...
  • Page 7 4-16KR Table of Contents Use as a robot-controlled Centrifuge ................ 37 6.3.1 Connection ....................37 6.3.2 Input ......................37 6.3.3 Serial Control Interface ................37 6.3.3.1 Introduction ................. 37 6.3.3.2 General specifications ..............38 6.3.3.3 Communication protocol ............. 38 User commands ................38 Control commands ...............
  • Page 8 4-16KR Table of Contents Disposal Disposal of the Centrifuge ..................63 Disposal of the Packaging ..................63 Technical Data 10.1 Ambient Conditions ....................64 Appendix 11.1 Suitable Accessories ....................65 11.2 Grafical Representation of the Rotors ............... 65 11.3 Formulae - Mathematical Relations ................66 11.3.1...

  • Page 9: General Information

    They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. SIGMA Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use. The intended use also includes...

  • Page 10: Copyright

    4-16KR General Information Copyright The copyright concerning the operating manual remains Sigma Laborzentrifugen GmbH. The operatin manual is solely intended for the operator and their personnel. It includes instructions and information that may not be duplicated, distributet, or communicated in any othe way neither in full nor in parts.

  • Page 11: Design Of The Centrifuge Overview

    4-16KR Design of the Centrifuge Design of the Centrifuge Overview Sliding cover Operating panel Mains switch Fig. 2.1: Total view of the centrifuge Name plate (see page 12) Fig. 2.2: Rear view of the centrifuge Serial interface Mains cable Version 10/2010, Rev. 1.9 of 02/07/2012...

  • Page 12: Name Plate

    4-16KR Design of the Centrifuge Name Plate Manufacturer and registered office Type name Serial number Max. speed Kinetic energy Max. density Nominal voltage Input fuse Symbol for special disposal (see chapter 9) CE mark in accordance with the directive 94/9/EC...

  • Page 13: Safety

    4-16KR Safety Safety Marking of the Unit International symbols used for SIGMA centrifuges: Gefährliche elektrische Spannung Dangerous voltage Courant haute tension Achtung, Betriebsanleitung lesen Attention, consult instruction manual Attention, consulter mode d'emploi Ein (Netzverbindung) On (Power) Marche (mise sous tension)

  • Page 14: Explanation Of The Symbols And Notes

    4-16KR Safety Explanation of the Symbols and Notes This operating manual uses the following names and symbols to indicate hazards: This symbol stands for a direct hazard to the life and health of persons. Non-observance of these symbols causes serious health problems up to life-endangering injuries.

  • Page 15: Responsibility Of The Operator

    4-16KR Safety Responsibility of the Operator The operator is responsible to authorise only qualified personnel to work on the machine (see chapter 3.4 "Operating Personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.

  • Page 16: Safety Instructions

    Stop the centrifuge immediately in the event of a malfunction (see chapter 7 "Malfunctions and Error Correction") or contact the service of SIGMA Laborzentrifugen GmbH (see 7.3 "Service Contact"). Ensure that all repairs are performed only by authorized and specialized personnel (see 7.3 "Service Contact").

  • Page 17: Safety Instructions For Robot-Controlled Centrifuges

    4-16KR Safety Prior to any start-up, check the centrifuge, rotor, and accessories for signs of damage that can be discerned from the outside. Special attention must be paid to all of the rubber parts (e.g. motor cover, lid seal, adapter) in terms of visible structural changes.

  • Page 18: Safety Instructions For Centrifugation

    4-16KR Safety 3.6.6 Safety Instructions for Centrifugation For safe operation, observe the following before starting the centrifuge: Ensure that the centrifuge was set up properly (see chapter 5 "Set-up and Connection“). Maintain a safety distance of at least 30 cm (12 inches) around the centrifuge.

  • Page 19: Safety Devices

    4-16KR Safety bucket with the engraving “max cycles = 10,000” has a service life of 10,000 cycles, and a rotor with the engraving “Exp.Date 02/15” must be scrapped in Feburary 2015 at the latest (see fig. 3.1)! Fig. 3.1: Different service life – engraving on the bucket/rotor...

  • Page 20: Ground Wire Check

    4-16KR Safety 3.7.4 Ground Wire Check For the ground wire check, there is an equipotential bonding screw on the rear panel of the centrifuge. A ground wire check can be carried out by authorized and specialized personnel using a suitable measuring instrument.

  • Page 21: Storage And Transport Dimensions And Weight

    4-16KR Storage and Transport Storage and Transport Dimensions and Weight Height: 426 mm Width: 670 mm Depth: 650 mm Weight: 134 kg Fig. 4.1: Dimensions and weight Storage Conditions The centrifuge can be stored for up to a year without any problems.

  • Page 22: Set-Up And Connection Unpacking The Centrifuge

    4-16KR Set-up and Connection Set-up and Connection Unpacking the Centrifuge The centrifuge is packaged in a wooden crate. After taking off the lid, remove the side panels. Remove the packaging material. Lift the centrifuge upwards with a lifting device to lift it safely.

  • Page 23: Installation Site

    4-16KR Set-up and Connection Installation Site Operate the centrifuge only in closed and dry rooms. All the energy supplied to the centrifuge is converted into heat and emitted to ambient air. Ensure sufficient ventilation. Keep a safety distance of at least 30 cm around the centrifuge so that the vents in the centrifuge remain fully effective.

  • Page 24: Using The Centrifuge Initial Start-Up

    4-16KR Using the Centrifuge Using the Centrifuge Initial Start-Up Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 "Set-up and Connection"). 6.1.1 Switching the Centrifuge ON Press the mains power switch on the right side of the front (see Fig.

  • Page 25: Installation Of Accessories

    4-16KR Using the Centrifuge When using rotors for microtiter plate formats: Ensure that the plate holders are inserted together with the plates into the bucket. Follow the safety instructions and hazard warnings in chapter 3! 6.1.3.2 Installation of Accessories Only use inserts that are suitable for the rotor (see 11.1 "Suitable Accessories").

  • Page 26: Adapters

    4-16KR Using the Centrifuge 6.2.2.1 Adapters In order to ensure easy handling, even if vessels of various sizes are used, carrier systems were developed. Load the opposite carriers with the same number of vessels and with the same weights in order to avoid imbalance.

  • Page 27: Spincontrol Universal - Manual Operation

    4-16KR Using the Centrifuge Spincontrol Universal – Manual Operation Although the centrifuge is designed for robot-controlled operation, manual operating is possible. The funcitons are described in detail in the following chapters. 6.2.1 Operating Panel Start key Centrifuge display (see Lid key...

  • Page 28: Starting The Centrifuge

    4-16KR Using the Centrifuge 6.2.3 Manual Mode 6.2.3.1 Starting the Centrifuge The centrifuge is ready for operation when the start key is illuminated. Press the start key in order to start a centrifugation run. 6.2.3.2 Interrupting a Centrifugation Run Press the stop key in order to interrupt a centrifugation run. The centrifugation run will be terminated prematurely.
  • Page 29 4-16KR Using the Centrifuge Press the right function key in order to confirm the input. If this is not done, the value will be automatically reset to the last setting. It is also possible to change the runtime during the centrifugation run.

  • Page 30: Speed

    4-16KR Using the Centrifuge Continuous Run During the continuous run, the runtime of the centrifuge is unlimited and must be stopped manually. The centrifuge accelerates during the continuous run until the set speed is reached. Starting with the time setting 0:10, turn the knob counter-...

  • Page 31: Relative Centrifugal Force (Rcf)

    4-16KR Using the Centrifuge 6.2.3.7 Relative Centrifugal Force (RCF) The relative centrifugal force (RCF) is the acceleration, to which the sample is subjected. The set value of this parameter is displayed in the upper section of this field, with the actual value shown below. The values are stated in g (gravitational acceleration) and depend on the speed value (see 11.3 "Formulae –...
  • Page 32 4-16KR Using the Centrifuge Press the right function key to confirm the selected value. If this is not done, the value will be automatically reset to the last setting. Fig. 6.11: Temperature preselection The temperature scale shows the actual temperature in the rotor chamber.
  • Page 33 4-16KR Using the Centrifuge Rapid cooling program "RAPID TEMP" Precooling at a standstill may distort the measurement results and subsequently cause increased wear of the mechanical components. This is why the centrifuge has a special program that precools the centrifuge rapidly under defined conditions: Turn the left function key until “run”...

  • Page 34: Rotor Selection

    4-16KR Using the Centrifuge 6.2.3.9 Rotor selection The inserted rotor mus be selected manually when the centrifuge is at a standstil. Rotor selection: Turn the left function key until “set” appears in the display field in front of the parameter "Rotor" (Fig. 6.15).

  • Page 35: Calling Up Stored Programs

    4-16KR Using the Centrifuge The settings are now saved under this program number (program number 08 in the example). Fig. 6.16: Saving the current settings 6.2.4.2 Calling Up Stored Programs Turn the left function key until “run” appears on the display (Fig.

  • Page 36: Activating/Deactivating The Sound Signal

    4-16KR Using the Centrifuge The current status remains active even if the centrifuge is switched off with the help of the mains power switch. fig. 6.18: Padlock symbol 6.2.6 Activating/Deactivating the Sound Signal If this function is active, a sound signal can be heard at the end of the operation as well as in the event of an error message or imbalance.

  • Page 37: Use As A Robot-Controlled Centrifuge

    6.3.3.1 Introduction This document describes the hardware specification and software protocol to communicate with a serial RS232 connection to a sigma centrifuge with Spincontrol electronics. The serial interface offers the possibility of firmware updates (by service technician), control and monitoring of centrifuge parameters and also the readout of service data like error list and cycles.

  • Page 38: General Specifications

    4-16KR Use as a robot-controlled Centrifuge 6.3.3.2 General specifications Interface standard: RS232 Baud rate: 9600 Parity: Data bits: Stopbits: Data format: ASCII The serial communication works without hardware- or XON/XOFF software handshake. 6.3.3.3 Communication protocol User commands consist of an ASCII-coded command string and - if needed - a parameter separated by a space.

  • Page 39: Control Commands

    4-16KR Use as a robot-controlled Centrifuge Control commands These commands cause an immediate action. start starts the centrifuge with the set values stop stops the centrifuge with the preadjusted deceleration fstop stops the centrifuge with the maximum deceleration door opens the door (only possible when the rotor is...

  • Page 40: Commands To Request The Status

    4-16KR Use as a robot-controlled Centrifuge Commands to request the status status requests the status of the centrifuge. The value is displayed decimal. Value Normal centrifuge or with Centrifuge with hatch in the lid motor driven lid Rotor is spinning or door is Rotor is spinning and the centrifuge opening / closing.

  • Page 41: Other Commands

    Part No.: 70926, Version 001 Software Part No.: 26490 – Software Version: 009 CompilationDate: Mar 27 2007 (14:16:22) TotalCycles: 70 TempOffset: -8 ImbalOffset: -1 SIGMA Laborzentrifugen GmbH Osterode www.sigma-zentrifugen.de Para Code Timestamp Version 10/2010, Rev. 1.9 of 02/07/2012 Translation of the original operating manual...
  • Page 42 4-16KR Use as a robot-controlled Centrifuge or this (6-16K): info Cent: 6-16KR 90583 Dev: Z4P Controlboard 71111 Ver: SW PN: 97116 SW Ver: 016 comp: Mar 16 2010 (11:16:23) The exact output format of this command may vary between different centrifuge types.
  • Page 43 [curveNr], [Lin/Quad], [Int1Time], [Int1Speed], [Int2Time], [Int2Speed],etc. Notice this command is only possible if no free curve is running! SIGMA> setcurve 22,0,130,112,60,148,60193,60,256,60326,60,524,60,1010 SIGMA> getcurve 22 CurveNr: 22 Interval 1: Time: 130 Speed: 112 LIN Interval 2: Time: 60 Speed: 148...
  • Page 44 4-16KR Use as a robot-controlled Centrifuge While there was no spin since the last reset, the centrifuge ID and string “No data available” will be output, else data will be output as follows: Item column column column Condition Centrifuge ID...
  • Page 45 4-16KR Use as a robot-controlled Centrifuge Item column column column Condition Run Time Run time Only if normal run started Infinite Run time set to infinite abcd hours, Output depends ef minutes, on set run time gh seconds Interrupted Stop button...
  • Page 46 4-16KR Use as a robot-controlled Centrifuge Item column column column Condition If started abcde *g Depends on set Speed was partly Speed error out of setting detected by run observation Not Reached Set RCF was not reached Not reached yet...
  • Page 47 4-16KR Use as a robot-controlled Centrifuge Item column column column Condition Deceleration Brake If started Curve 9 (Short Short run run) Curve 9 (Fast Fast stop stop) Curve x Normal run, output depends on set deceleration curve Changed during Set deceleration...

  • Page 48: Table Of User Commands

    4-16KR Use as a robot-controlled Centrifuge Table of User Commands The following table contains the available user commands. Command Return 2. name Parameters Unit Format name values start stop fstop door reset reseterr setspeed OUT_SP_1 UINT settemp OUT_SP_2 °C settime OUT_SP_3 sec.

  • Page 49: 6.3.3.4 Examples

    4-16KR Use as a robot-controlled Centrifuge 6.3.3.4 Examples All commands have to be sent without quotation marks and brackets! [CR] and [LF] are ASCII coded control characters (Carriage Return and Linefeed). setting the setspeed to 1000 rpm ‘setspeed 1000[CR][LF]’ starting the centrifuge ‘start[CR][LF]’...

  • Page 50: Commands For Centrifuges With Motor Driven Lid Or Hatch

    4-16KR Use as a robot-controlled Centrifuge Commands for centrifuges with motor driven lid or hatch closes the lid / hatch close[CR] Commands for centrifuges with rotor positioning n=0: unlock the rotor setpos n[CR] n>0: go to position n the lid must be close for positioning If the centrifuge is running, this command stops the run automatically and the rotor goes to position n.

  • Page 51: Hardware Interface (Optional Accessory)

    4-16KR Use as a robot-controlled Centrifuge 6.3.3.5 Hardware interface (optional accessory) Pinning of the connector Pinning of the 9 pin SUB-D (male) connector on the cover of the centrifuge PIN 2: TxD (transmit) PIN 3: RxD (receive) PIN 5: GND (ground)

  • Page 52: Rotors

    4-16KR Use as a robot-controlled Centrifuge 6.3.4 Rotors Robot-controlled centrifuges require special rotors that are equipped with magnets on the bottom surface (Fig 6.19). They are necessary in order to ensure the correct position of the rotor. When the desired position is reached, the rotor is locked mechanically.

  • Page 53: Malfunctions And Error Correction

    4-16KR Malfunctions and Error Correction Malfunctions and Error Correction General Malfunctions Malfunctions are indicated by error messages with a number in the speed display. If the sound signal is activated, it sounds when the error message is displayed Eliminate the source of the problem (see tables in fig. 7.1 and 7.3).

  • Page 54: Emergency Lid Release

    4-16KR Malfunctions and Error Correction 7.1.1 Emergency Lid Release In the event of a power failure, it is possible to manually open the centrifuge lid. Switch off the mains power switch and disconnect the power cord from the socket. Remove the plug (1) from the opening on the left side of the control panel, e.g.

  • Page 55: Error Codes

    4-16KR Malfunctions and Error Correction Error Codes Error no. Kind of error Measure Note System error All these errors stop the allow to slow down centrifuge or cause it to power off/on decelerate brakeless 10-19 Speedometer error allow to slow down...

  • Page 56: Service Contact

    Germany: use the service request form at www.sigma-zentrifugen.de [Service Area] or contact SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-8425 Fax +49 (0) 55 22 / 50 07-9425 E-mail: service@sigma-zentrifugen.de...

  • Page 57: Maintenance

    4-16KR Disposal Maintenance 8.1.1 Centrifuge Disconnect the power cord from the wall outlet or instrument receptacle before cleaning. Carefully remove all liquids, including water and particularly all the solvents, acids, and alkaline solutions from the rotor chamber using a cloth in order to avoid damage to the motor bearings.

  • Page 58: Accessories

    4-16KR Maintenance and Service 8.1.2 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at the same time. Immediately rinse off the rotor, buckets, or accessories under running water if they have come into contact with any liquids that may cause corrosion.

  • Page 59: Load-Bearing Bolts

    4-16KR Disposal Check the material regularly (at least once a month) for - cracks - visible damage of the surface - pressure marks - signs of corrosion - other changes. Check the bores of the rotors and multiple carriers. Replace any damaged components immediately for your own safety.

  • Page 60: Condenser

    4-16KR Maintenance and Service 8.1.6 Condenser In order to cool the refrigerant that is compressed by the refrigeration unit, a lamellar condenser is used. It is cooled by air. Dust and dirt obstruct the cooling flow of air. Dust on condenser pipes and lamellas reduces the heat exchange and thus the performance of the refrigeration unit.

  • Page 61: 8.3 Service

    4-16KR Disposal Accessories max. temp. min. time max. time max. (°C) cycles Glass tubes 134-138 Polycarbonate tubes 115-118 Polypropylene copolymer tubes 115-121 Teflon tubes 134-138 Aluminium rotors 134-138 Polycarbonate/Polyallomer lids for angle rotors 115-118 Polysulfone lids for angle rotors 134-138...

  • Page 62: Return Of Defective Parts

    Germany: use the service request form at www.sigma-zentrifugen.de [Service Area] or contact SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-8425 Fax +49 (0) 55 22 / 50 07-9425 E-mail: service@sigma-zentrifugen.de...

  • Page 63: Disposal

    This symbol means that it is not permissible to dispose of the unit among household trash. You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH. Ensure that the unit is decontaminated. Fill in a declaration of decontamination.

  • Page 64: 10 Technical Data

    4-16KR Technical Data 10 Technical Data Manufacturer: S I G M A Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: 4-16KR Electr. Connection: see name plate Protection class: IP Code: Connected load (kVA): Power consumption (kW): Max. current consumption (A):...

  • Page 65: 11 Appendix

    For use in a robot-controlled centrifuge, special equipment is required. A list of suitable rotors and accessories is available on request. Please contact our sales department.: SIGMA Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-8213...

  • Page 66: 11.3 Formulae - Mathematical Relations

    4-16KR Appendix 11.3 Formulae - Mathematical Relations 11.3.1 Relative Centrifugal Force (RCF) The parameters speed, RCF, and the group rotor and radius cannot be specified independently. They are interrelated via the following formula: RCF = 11.18 x 10 x r x n If two values are given, the third value is determined by the equation.
  • Page 67 4-16KR Appendix Fig. 11.2: Speed-Grravitational-Field Diagram Version 10/2010, Rev. 1.9 of 02/07/2012 Translation of the original operating manual...

  • Page 68: Resistance Data

    4-16KR Appendix Resistance Data Resistance Data Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated Acetone Acrylonitrile Allyl alcohol Aluminum chloride AlCl saturated Aluminum sulfate Ammonium chloride...
  • Page 69 4-16KR Appendix Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Dioxane Dipropylene glycol (mono)methyl ether Ethyl acetate Ethylene chloride Ferrous chloride FeCl saturated Formaldehyde solution Formic acid Furfural...
  • Page 70 4-16KR Appendix Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Potassium hydrogen carbonate CHKO saturated Potassium hydroxide Potassium hydroxide Potassium nitrate Potassium permanganate KMnO Pyridine Resorcinol Silver nitrate...

  • Page 71: Ec-Declaration Of Conformity

    EC-Declaration of Conformity...
  • Page 73 4-16KR Index 12 Index Acceleration time - see also the softstart and softstop function ..............28 Accident prevention ......................15 Acid ........................18, 57, 68, 70 Activating/Deactivating the sound signal ................36 Actual temperature....................... 32 Adapters ........................26, 59 Alkaline solutions ......................18, 58 Aluminium accessories ......................
  • Page 74 4-16KR Index Contamination ........................ 17, 57 Continuous heat resistance ....................60 Continuous run ........................30 Control commands ....................... 39 Copyright ..........................10 Corrosion ....................16, 18, 26, 56, 58, 59 Cost estimate ........................63 Cracks ........................18, 58, 59 Cycles ..........................18, 24 Damage of the surface ......................
  • Page 75 4-16KR Index Hardware interface (optional accessory) ................51 Hardware specification ......................37 Hazard warnings ......................9, 25 Hexagon socket wrench ....................... 10 Imbalance ......................18, 25, 26, 59 Imbalance error ........................55 Imbalance monitoring ......................20 Importance of the operating manual ..................9 Important information ......................
  • Page 76 4-16KR Index Measures in the event of hazards and accidents..............20 Mechanical safety ........................ 16 Motor error ........................... 55 Motor shaft ........................24, 57 Multiple carrier ......................26, 58, 59 Name plate ......................11, 12, 16, 23 No indication on the display ....................53 No power in the mains supply ....................
  • Page 77 4-16KR Index Remove glass particles and metal dust from the rotor chamber ........... 59 Resistance data ....................... 18, 58, 68 Resistance of plastics ......................18 Responsibility of the operator ....................15 Return of centrifuges, spare parts, or accessories ............... 62 Return of defective parts ......................
  • Page 78 4-16KR Index Starting the centrifuge ......................28 Status of the centrifuge ......................37 Sterilization and disinfection of the rotor chamber and accessories........60 Storage and transport ......................21 Storage conditions ....................... 21 Storage temperature ......................21 Stress-corrosion ........................58 Structural changes ....................... 16 Suitable accessories ......................

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