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Sigma 3-16PK Use And Maintenance Manual
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Sigma 3-16PK Use And Maintenance Manual

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Standard Operating Procedure
Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK
Location:
CBE Laboratory Unit (H21/22)
1PURPOSE
To describe procedures for the safe operation and maintenance of the bench standing Sigma 3-16PK
refrigerated centrifuge.
2 SCOPE
This SOP applies to the use and maintenance of Sigma 3-16PK centrifuge by authorised CBE
personnel within CBE laboratories. The centrifuge is used for separating substances or mixtures with a
density of up to max 1.2g/cm
designated rotors; swing-out rotor for sealable buckets (Cat.-No. 11180) and swing-out rotor for plate
centrifugation (Cat.-No. 11222). This SOP does not cover the use of any other type of rotor with this
centrifuge. Certified and hermetically sealed accessories (e.g. rotors, buckets or safety cups) MUST be
used for applications involving the centrifugation of infectious, toxic or pathogenic material
SPECIAL NOTES: HEALTH & SAFETY
2.1
• Mechanical failure of rotating parts (often violent).
• Contact with rotating parts.
• Sample leaks causing aerosols, stress corrosion, contamination.
• Sample imbalance causing machine movement/walking.
• Fire/explosion.
• Contact with contaminated components/vapours.
2.2
Safe Working Practices (also refer to Safety Note in Operator Manual)
Users MUST read all Precautionary Statements in Section 2 of the Operators Manual and should
refer to the Guidelines in Annex 1 for further recommendations on the safe use of centrifuges.
(i)
Ensure that the centrifuge is placed on a horizontal level surface and there is a clearance of at
least 30 cm around the centrifuge when in operation. Make sure that hazardous materials are
not stored or placed within this area and there is sufficient ventilation.
(ii)
The centrifuge should not be placed in a safety cabinet unless an operator protection factor (KI
Discus) test has been carried out with it running in situ and it shown not to compromise operator
protection.
(iii)
Always observe the start-up checks detailed in this SOP before using the centrifuge
Version 003
Written by: Victoria Workman
Centre for Biological Engineering
3
. The SOP covers the use of the centrifuge using the following
Hazards associated with use of centrifuge
Effective Date: 26
Reviewed by: P.Hourd
CONTROLLED COPY
th
June 2018
Page 1 of 22
SOP122
Review 26
Approved by: R/I.Temple
th
June 2020

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Summary of Contents for Sigma 3-16PK

  • Page 1 Location: CBE Laboratory Unit (H21/22) 1PURPOSE To describe procedures for the safe operation and maintenance of the bench standing Sigma 3-16PK refrigerated centrifuge. 2 SCOPE This SOP applies to the use and maintenance of Sigma 3-16PK centrifuge by authorised CBE personnel within CBE laboratories.
  • Page 2 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (iv) Do not centrifuge toxic, pathogenic or radioactive material without taking proper precautions for aerosol containment.
  • Page 3 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (xix) Check that the replacement of time-expired components are made according to the manufacturer’s Instructions - Do not use rotors or buckets which have exceeded their maximum operating life.

  • Page 4: Equipment And Materials

    CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (v) Shall schedule servicing and preventative maintenance with authorized service representatives. External maintenance and servicing of the equipment can only be performed after it has been suitably disinfected (see SOP003) and a 'Decontamination Certificate' has been issued (a proforma is available on the CBE website).
  • Page 5 Make certain the rotor tie down nut is secure before starting the centrifuge. Check that the rotor is seated on the drive hub correctly. (iv) Inspect the supporting lugs on the rotor; lubricate if required (use Sigma grease Part no. 70 284). Fill sample containers only within the range recommended by the manufacturer. Never fill sample containers when they are mounted in the centrifuge unless contained in a head specifically designed for this method of filling.
  • Page 6 Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (i) Swing out rotor (part number: 11180) takes a maximum of 1100g in each of the four buckets.
  • Page 7 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (iii) Insert the holders into the rotor, ensuring that the holders fit correctly into the trunions. Always use both holders 5.3 Display/ program options...
  • Page 8 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) The centrifuge display has the following display fields: 1 Time field 2 "Lock" symbol for lockdown...
  • Page 9 Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (vi) To change the time in steps of 1 second (instead of in steps of 10 seconds in the min:sec mode) or in steps of 1 minute (instead of in steps of 10 minutes in the hrs:min mode): Keep the stop key pressed while setting the desired runtime with the right rotary knob.
  • Page 10 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) 5.3.5 Setting the temperature The temperature of the centrifuge is controlled by a refrigeration unit. Temperatures between -10 °C and + 40 °C can be preselected.
  • Page 11 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) 5.3.5.1 Using the Rapid cooling facility Pre-cooling at a standstill may distort the measurement results and subsequently caused increased wear of the mechanical components.
  • Page 12 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (ii) To avoid corrosion, do not expose aluminium rotor components to strong acids or bases, alkaline lab detergents, or salts (chlorides) of heavy metals (e.g., caesium, lead, silver or mercury).

  • Page 13: Every Month

    CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) 5.5.3. Routine Checks of Rotors and Accessories EVERY MONTH: This is the responsibility of the housekeeper as part of the housekeeping duties.
  • Page 14 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) adaptors should be removed after use to allow adequate cleaning and drying. Ensure that the manufacturer's recommendations on cleaning materials are followed.
  • Page 15 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (ii) Check Operator’s Manual to make sure that individual materials are resistant to this steam treatment and for recommended sterilisation cycles.
  • Page 16 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) (ii) All problems and corrective actions should be recorded in the Preventative Maintenance of centrifuges Record (Section 6).
  • Page 17 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) Annex 1: Safe Use of Centrifuges (Extracts from BS4402) Moving parts in centrifuges can reach very high kinetic energies. Breakage of a rotor or accessories inside the centrifuge releases large amounts of energy, and debris can be expelled at high speeds from the centrifuge;...
  • Page 18 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) B.1.4.2 Fill sample containers and head accessories only within the range recommended by the manufacturer and avoid contamination outside the seal of a sealed head or sealed bucket.
  • Page 19 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) B.1.6.3 Avoid moving defective and damaged components, unless it is necessary for decontamination of hazardous materials, and in any event retain all components and debris for inspection by the manufacturer's representative.
  • Page 20 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) 7. Over speeding because de rated over speed disc not fitted. 8. Running badly corroded and weakened rotors or buckets; stress corrosion failure.
  • Page 21 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) Remember that it is important to follow the manufacturer's instructions in all aspects of centrifuge use. Only trained persons should use centrifuges. If in doubt about the operation of a centrifuge –...
  • Page 22 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP122 Title: Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK Location: CBE Laboratory Unit (H21/22) SOP Version History Version Date Revised/ Revision Summary Reviewed Reviewed Version Number October i)Transferred to the new lean template with minor...