Thermo Scientific Heracell 150i GP Operating Instructions Manual page 39

Chapter 4 Description of the Device
Under normal operating conditions and at the usual incubation temperature of 37 °C, a constant
relative humidity of approx 93 % is achieved in the work space.
If dew formation occurs on the culture containers due to elevated relative humidity, the humidity in
the work space can be adapted to a lower value by enabling the Low Humidity feature. The relative
humidity in the work space is lowered from approx. 93 % to approx. 90 %. The modification
requires an extended adaption phase. To ensure that it effectively prevents dew formation on
culture containers, it must be used as a permanent setting.
CO supply:
2
To ensure the growth conditions for the cell and tissue cultures, the work space is supplied with
CO .
2
The pH of the bicarbonate-buffered culture media largely depends on the CO
space atmosphere.
The CO -content of the work space atmosphere can be controlled within a range of 0 to 20 %.
2
The supplied CO must have one of the following quality characteristics:
2
• Purity 99.5 % min.
• medical gas quality
O supply:
2
If the CO -incubator is to be operated with more than 21 % oxygen, the work space is supplied
2
with oxygen. The O
0 to 21 %.
N supply:
2
If the oxygen content during operation is to be lowered to less than 21 % (air oxygen content), the
work space is supplied with nitrogen. The O
controlled depending on the configuration of the sensor within a range of 1 % to 21 % and 5 % to
90 %.
Heracell 150i / 240i GP 50159351_B_06-2020
-content of the work space atmosphere can be controlled within a range of
2
-content of the work space atmosphere can be
2
32
-content of the work
2
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