Summary of Contents for Thermo Scientific 1500 A2 Series
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Class ll, Type A2 Biological Safety Cabinet 1500 Series A2, Made in the Operating manual 7021555 • Revision 0 • 5/17/24...
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IMPORTANT Read this instruction manual. Failure to read, understand and follow the instructions in this manual may result in damage to the unit, injury to operating personnel, and poor equipment performance. CAUTION All internal adjustments and maintenance must be performed by qualified service personnel. Material in this manual is for information purposes only.
1.1 About this Manual The Thermo Scientific™ 1500 Series Class II, Type A2 biological safety cabinet has been tested and certified in accordance to NSF/ANSI 49, and is designed to protect the user, the environment, and your research from harmful substances and cross-contamination.
1.2 Intended Use The 1500 Series Class II, Type A2 biosafety cabinet is for installation and operation in microbiological and biotechnical laboratories of safety levels 1, 2 and 3 and offers protection for personnel, product, and environment in laboratories and similar environments working within biological hazards.
1.4 Regulatory Compliance 1.4.1 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EC. It is marked with the adjacent symbol. Thermo Fisher Scientific has contracted with companies for recycling/disposal in each EU Member State. For further information, email to weee.recycle@thermofisher.com.
2 Technical Specifications Table 1. Biological Safety Cabinet Technical Specifications for 4 ft & 6 ft models Dimensions 4 ft Models 6 ft Models (10 inch window opening work position) (10 inch window opening work position) 1544A2, 1545A2, 1554A2, 1555A2, 1547A2, 1549A2, 1557A2, 1559A2, 1577A2, 1575A2, 1576A2, 1585A2, 1586A2 1578A2, 1587A2, 1588A2...
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Table 1. Biological Safety Cabinet Technical Specifications for 4 ft & 6 ft models (Continued) Dimensions Remaining lower ports - Distance lower edge 353.1 / 13.9 (mm / in) Distance from back panel SmartPort (mm / in) 182.9 / 7.2 First access port 259.0 / 10.2 (mm / in)
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Table 2. Biological Safety Cabinet Technical Specifications for 3 ft & 5 ft models Dimensions 3 ft Models 5 ft Models (10 inch window opening work position) (10 inch window opening work position) 1520A2, 1521A2, 1522A2, 1523A2, 1568A2, 1569A2, 1570A2, 1571A2, 1580A2, 1530A2, 1531A2, 1532A2, 1533A2 1581A2, 1582A2, 1583A2 Description...
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Table 3. Volume, Weights, and Loads Specifications for 4 ft & 6 ft models Volume, weights, and loads 4 ft Models 6 ft Models (10 inch window opening work position) (10 inch window opening work position) 1544A2, 1545A2, 1554A2, 1555A2, 1547A2, 1549A2, 1557A2, 1559A2, 1577A2, 1575A2, 1576A2, 1585A2, 1586A2 1578A2, 1587A2, 1588A2...
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Table 4. Volume, Weights, and Loads Specifications for 3 ft & 5 ft models Volume, weights, and loads 3 ft Models 5 ft Models (10 inch window opening work position) (10 inch window opening work position) 1520A2, 1521A2, 1522A2, 1523A2, 1568A2, 1569A2, 1570A2, 1571A2, 1580A2, 1530A2, 1531A2, 1532A2, 1533A2 1581A2, 1582A2, 1583A2...
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Table 5. Electrical Data for 3 ft & 5 ft models and 4 ft & 6 ft models Electrical data 120 V Models - 4 ft / 6 ft 230 V Models - 4 ft / 6 ft 1505A2, 1507A2, 1525A2, 1527A2, 1506A2, 1508A2, 1526A2, 1528A2, 1536A2, 1535A2, 1537A2, 1545A2, 1555A2, 1538A2, 1544A2, 1549A2, 1554A2, 1576A2,...
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Table 6. Airflow System for 4 ft & 6 ft models Airflow system Description 4 ft Models 6 ft Models (10 inch / 8 inch window opening work (10 inch / 8 inch window opening work position) position) 1505A2, 1506A2, 1525A2, 1507A2, 1508A2, 1527A2, 1528A2, 1537A2, 1526A2, 1535A2, 1536A2, 1544A2, 1538A2, 1547A2, 1549A2, 1557A2, 1559A2,...
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Table 6. Airflow System for 4 ft & 6 ft models (Continued) Airflow system Length mm / in 610 / 24 915 / 36 Depth mm / in 117 / 4.61 Biological Safety Cabinet 1500 Series A2 2 Technical Specifications |...
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Table 7. Airflow System for 3 ft & 5 ft models Airflow system Description 3 ft Models 5 ft Models (10 inch / 8 inch window opening work (10 inch / 8 window opening work position) position) 1520A2, 1521A2, 1522A2, 1550A2, 1551A2, 1552A2, 1553A2, 1566A2, 1523A2, 1530A2, 1531A2, 1532A2, 1567A2, 1568A2, 1569A2, 1570A2, 1571A2,...
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Table 7. Airflow System for 3 ft & 5 ft models (Continued) Airflow system Length mm / in 610 / 24 Depth mm / in 117 / 4.61 Biological Safety Cabinet 1500 Series A2 2 Technical Specifications |...
3 Description Figure 1. Unit Components The Thermo Scientific 1500 Series A2 bio safety cabinet offers innovative SmartFlow™ Plus technology; an automatic airflow compensation system that adjusts motor speed as filters load, without the use of a manual damper. The SmartFlow™ Plus system ensures safe working conditions, even between annual certifications.
Table 8. Standard Configuration: Internal Outlets as Accessory Power Source Two duplex right and left side Two single right and left side 1505A2, 1507A2, 1520A2, 1521A2, 1522A2, 1506A2, 1508A2, 1526A2, 1528A2, 1530A2, 1531A2, 1523A2, 1525A2, 1527A2, 1535A2, 1537A2, 1532A2, 1533A2, 1536A2, 1538A2, 1544A2, 1549A2, 1539A2, 1540A2, 1541A2, 1542A2, 1545A2, 1554A2, 1559A2, 1566A2, 1567A2, 1572A2, 1573A2, 1547A2, 1550A2, 1551A2, 1552A2, 1553A2,...
3.1.6 Window position monitoring The position sensors detect the size of the front window opening and indicate whether the window is open to the specified work position, closed (energy saving) or in an unsafe intermediate position. 3.2 HEPA Filters Figure 2. Airflow Filtering System The filter system consists of two HEPA filters for downflow (supply) and exhaust air.
3.4 Use of the Window The sample chamber is accessible through various positions of the front window. The working position of the window is an 8" or 10" opening, +0.25”, -0.75” (depending on model - check data label and first page of this manual). For loading the chamber, the window opens fully.
Figure 5. Window Ready Figure 6. Standby Mode Figure 7. Window Open Our patented SmartClean™ window design easily lowers below the work position for replacing the sample chamber light bulbs (See Figure 3). CAUTION: Do not use force, if the front window gets jam or is difficult to move. Contact Technical Services immediately and do not attempt to repair the problem yourself.
Figure 8. Interfaces (2 of 4 access ports are shown) 3.6 Chamber Lighting The Thermo Scientific 1500 Series A2 bio safety cabinet offers a bright workspace for a more comfortable working environment. Models with coated rear and side walls resist glare and make it easier for the user to work safely.
Figure 9. UV Light and Chamber Light Note: To protect from UV radiation, the UV light can only be turned on when the window is completely closed. 3.8 Work Area A single piece work tray is provided with the cabinet. It is placed onto the frame above the sample chamber drain pan. It can be removed by lifting up, using the large holes in the work tray.
4 Installation The operational safety and proper function of the unit depends on the location where it is to be operated. The cabinet should be operated only in the ambient conditions listed below. • Up to 6,562 ft (2000 m) above sea level. •...
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Figure 11. Thimble 4.2.1 Connecting Sleeve 1. Place the connecting sleeve onto the connecting opening of the accessory and secure it with the screws. 2. Fit the connecting pipe [200 mm (8 in)] of the on-site exhaust air system to the connecting sleeve. 3.
4.3 Moving the Unit To prevent tilting, always transport the cabinet separately from the stand, using equipment capable of supporting the weight, even when moving within a building. CAUTION: When moving the unit, lift only at the lift points as shown in Figure CAUTION: Do not allow the weight of the cabinet to rest on the drain pan.
4.4 Service Valve Connections Install the service valve(s) by removing the centers of the plug (brass) or piercing it at the pre-determined location (plastic). Installation spacing on side of unit is indicated in Figure 14. Note: The plugs must only be used for the installation of service valves in accordance with the applicable national regulations. Figure 14.
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Figure 15. Valve Assembly and Installation Biological Safety Cabinet 1500 Series A2 4 Installation |...
4.5 Universal Piping Connections Universal piping is a factory-installed option. The piping can be installed on either one or both the sides. Access points, depending on selected installation, are available on the top, side or bottom of the cabinet. Pipe threads are 1/4” FPT. Refer to Figure 16.
4.6 SmartPort™ SmartPort™ locations are indicated in Figure 16 (one on each side).The SmartPort™ feature on the 1500 Series A2, designed to improve work area organization inside the biological safety cabinet. It is designed to accommodate the routing of vacuum line tubing, line cords and data cables from the interior of the cabinet to the exterior working area, providing space inside the work area.
5 Start-Up CAUTION: Correct assembly and installation is essential for proper start-up and operation. 5.1 Assemble the Stand For a unit without stand, place the cabinet onto a stable structure that can support twice the weight of the unit. Make sure the weight is not resting on the drain valve or nipple.
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Figure 17. Stand Assembly 6. To install the cabinet frame to the stand, insert four flat washers and Allen screws loosely into the threaded holes at the bottom of the cabinet. 7. Place the safety cabinet onto the stand so that the Allen screws are routed through the holes of the keyhole tabs. 8.
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Table 10. Installing the Caster Stand Cabinet Caster Stand 1911584 Caster Stand (3 feet) 1911582 Caster Stand (4 feet) 1911585 Caster Stand (5 feet) 1911583 Caster Stand (6 feet) 1. Slide the two crossmembers (2) onto the retaining angles (3) of the side panels (1), then secure the crossmembers to the side panels using the screws (4) with fitted wedge lock washers (4a).
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Figure 19. Installing the Caster Stand Biological Safety Cabinet 1500 Series A2 5 Start-Up |...
5.2 Unlock Counterweight The window counterweight is secured to the back of the cabinet to protect the glass window from damage during shipping. CAUTION: The counterweight on the cabinet back compensates for the weight of the front window. Do not try to move the front window before the counterweight has been unlocked.
5.3 Level the Cabinet Note: The cabinet should be leveled only after it has been located in the desired working location. For cabinets without a stand, place a bubble level onto the work tray surface and adjust the cabinet until the level indicates an absolutely horizontal position in all directions.
CAUTION: Thermo Scientific will not warrant operational safety if the unit is operated without the required installation and certification test, or if these tests and repeat test are not performed by adequately trained and authorized personnel.
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Biohazard Cabinet Field Certifier Program 1. NSF International PO Box 130140 789 N. Dixboro Rd Ann Arbor, MI 48113-0140 Telephone (734) 769-8010 Or (800) NSF-MARK Fax (734) 769-0109 http://www.nsf.org/Certified/Biohazard-Certifier 2. IAFCA PO Box 12155 Columbus, OH 43212 Telephone (888) 679-1904 Fax (614) 486-1108 http://www.iafca.com/certifier.html The Controlled Environment Testing Association (CETA) is a trade association devoted to promoting and developing quality...
5.7 Rear Cover The rear cover is an optional feature on the 1500 Series A2, designed to improve efficiency of cleaning the biological safety cabinet. For 3 & 4 feet models, 2 pieces of sheet metal covers are provided. For 5 & 6 feet models, 3 pieces of sheet metal covers are provided.
6 Operation 6.1 Initial Setup This section explains how to set up the device using the initial setup routine. When the unit is turned on for the first time after delivery to the customer site, you must complete an initial setup process before you can use the unit.
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3. Tap the language button to turn blue to set the language (default is English). Tap the date button (MM/DD/YYYY) to open the keypad to set the date. Figure 24. Date Setup 4. Tap the time button (HH:MM) to open the keypad to set the time. Figure 25.
6.2 Operation This chapter describes how to control the Cabinets from the touchscreen display mounted in the light dome of the unit. The screen display unit measures 17.8 cm (7") diagonally across and has touch-sensitive areas that support finger gestures like tapping, it can be used with gloves.
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The Info & Health Status Area displays the following information: • Unit Name (top left in Figure 28) • Date/Time (bottom left in Figure 28), as entered during the initial setup (see 6.1 Initial Setup) or adjusted later on (see Display Setting).
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The screen content changes with operational state, depending on whether the unit is: in Startup mode, as shown in 6.3 Startup Mode • • in Working mode, as shown in 6.4 Working Mode • in Standby mode, as explained in 6.5 Standby Mode •...
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Table 13. Button and Indicator Meaning Indicator Meaning The Light Bulb button switches the interior sample chamber illumination of the biological safety cabinet on and off. The Fan button switches the device on and off. Tap and hold this button for 5 sec. to switch the device off (including the blowers) during the Standby mode.
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Note: The work mode of the unit determines whether the control panel button is operable or not. The unit will ignore the inop- erable icon tapping. Table 14. Operational Modes and Unit Functionality Window Work Standby Button OFF Mode Open UV Mode Mode Mode...
6.3 Startup Mode Figure 33 shows the Main Screen while the unit is starting up. The following startup screen shows what happens when the Fan button is tapped for turning on fans. The screen displays an alert due to the fact that the airflow speed has not reached the required level yet.
6.5 Standby Mode Figure 35 shows the Main Screen in standby mode. Figure 35. Main Screen - Unit in Standby Standby mode is entered when you close the window from within a working session. A Standby Mode header appears to show the unit is in standby mode, maintaining containment, and ready to resume work when you move the window back to the working position.
Figure 37 shows that it is in Idle mode with the window ready. In this case, the downflow and inflow status indicator boxes show an Alert icon. Figure 37. Main Screen - Idle Mode, Window Ready Figure 38 shows that it is in Idle mode with the window open. In this case, the window status indicator boxes show an Alert icon.
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6.7.1 Manual UV disinfection If the UV button is pressed while the window is open and/or the LED light is on, the following pop-up window shown below is displayed on the Main Screen: Figure 39. Main Screen - UV Disinfection rejected While the routine is running, the “UV disinfection”...
6.7.2 Auto UV Disinfection The UV disinfection can be started automatically with the specified time if the UV-auto-start function is enabled. Refer to 6.7 Running UV Disinfection. Note: When it is not in the main screen (standby mode) the UV disinfection will not be started automatically even though the specified time has reached, which means that the UV disinfection will be ignored once.
6.8.2 Replacing UV Bulb If you replace a new UV bulb, please follow below process to update the UV status Tap the Reset button to update the installed date, and the UV work hours will be fall to zero. • When you tap the Reset button, a pop-up screen shown in Figure 43.
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Tapping the Snooze button silences the audible alarm temporarily for a pre-set timeout period. (The default alarm snooze timeout period is 5 minutes, but the setting may be changed in the “Alarms and Alerts” screen, as explained in Snooze Timeout). The Alarm bell icon is crossed out to indicate that the audible alarm signal has been snoozed as shown in Figure 46.
6.10 Handling Alarms To handle the problem, proceed as follows: • Inspect the small blue alarm counter in the red Health Status Alarm icon to find out how many alarms you have to handle. • Tap the red Health Status Alarm icon to display the “Health Status Alarm” screen, then you can review the alarm list. Figure 47 is an example.
The Health Status Alert icon in the shape of a yellow Alert triangle icon appears on a black background. Additionally, the Health status icon has a small blue circle overlay that shows the number of pending alerts. If you tap the Alert triangle icon, you will be taken directly to the “Status Alert” screen with its alert list, as shown in Figure 49. Figure 49.
6.13 Settings This section explains how to set up the unit using the various options of the “Settings” screen. After completing the initial setup process, you can further set up and customize the unit by using the options of the “Settings” screen shown below in Figure 50. Figure 50.
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Figure 51. Display Setting Screen 6.13.2 Alarms and Alerts Snooze Time Setting Tapping the Alarms and Alerts button on the “Settings” screen takes you to the “Alarms and Alerts” screen, shown in Figure 52 below. From the “Alarms and Alerts” screen, you may change the audible alarms suspend time when Snooze button was tapped. Minutes are selectable from a drop-down list.
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6.13.3 UV Disinfection Tapping the UV Disinfection button takes you to the “Disinfection Settings” screen, shown in Figure 53 below. From this screen, you can change the default running time of the UV disinfection cycle. This value is used by default when you start the Disinfection cycle by tapping the UV light button in the Control Panel Area of the display window.
6.14 Alarm/Alert Log Tapping the Alarm/Alert log icon in Main Navigation Bar takes you to the “alarm/alert log” screen shown in Figure 54. You can browse up to 50 latest records of alarm/alert events. Figure 54. Alarm/Alert log Log file Screen 6.15 Data Plate Tapping the Data Plate icon in the “Settings”...
6.16 Loading the Chamber 1. Move the front window to the maximum opening position. This automatically switches the blowers to full speed. 2. Install needed work materials within the sample chamber work area. Avoid blocking the front air intake grille. 3.
• To compensate between the floor and sitting height, a footrest should be used. The minimal effective size of the footrest should be 18 x 14 in (45 x 35 cm). The slope should be adjustable to within a range from 5° to 15°. The adjustable height should extend to a minimum of 4 in (11 cm) above the floor.
6.19 Power Supply Connection The safety cabinet should remain connected to the power supply system at all times to ensure that settings for the individual unit configuration remain active in the memory. If the power supply is interrupted and later the power supply connection has been re-established, the system switches to the operating mode that had been active last.
7 Cleaning / Decontamination Several procedures can be used for decontaminating the biological safety cabinet. The procedure is selected, depending on the potential risk imminent in the agents used, and/or the degree of cleanliness required by an experiment or work process. One possible decontamination procedure is to clean, rinse and dry with a disinfection liquid or spray, and sterile distilled water.
PCBs and other components under the front canopy as they are susceptible to damage from the process. Thermo Fisher Scientific has developed validated procedures for the decontamination of the Thermo Scientific 1500 Series A2 using the Steris VHP (Vaporized Hydrogen Peroxide) system. These procedures are available on request.
7.7 Clean Exterior Surfaces Clean the exterior surfaces of the cabinet using a solution of tepid water and commercially available mild dishwashing agent. Then, dry all surfaces well, using a soft, clean cloth. 7.8 Clean Window For cleaning, the window can be lowered beyond the closing position (3.4 Use of the Window). Creating a gap at the upper edge of the window ensures that the upper portion of the window can be cleaned and/or disinfected.
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Figure 58. Protective Paper Catch Grid Biological Safety Cabinet 1500 Series A2 7 Cleaning / Decontamination |...
8 Maintenance & Service 8.1 Maintenance The annual certification consists of the following checks: • Electrical safety in accordance with national regulations. • Functional test of the device. • Checking all components for possible damage. • Checking the filter state. •...
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8.2.1 Sample Chamber Lights The sample chamber is illuminated by two (3, 4, 5 & 6 ft units) LED bulbs. The LED bulbs are installed in the light mounting frame in the sample chamber (see Figure 59). Note: Orientation of the LED bulb should be in such a way that LED strip inside LED tube should not be oriented towards work tray.
8.3 Replacements and Repairs CAUTION: Remote alarm systems can be retrofitted and integrated into the unit controls. Alarm contacts change state based on window position. Airflow changes from window position could impair personnel and product protection. Installation of these systems should only be performed by authorized service personnel. 8.4 Unit Disposal The entire cabinet with the exception of the HEPA filters can be discarded after having been thoroughly cleaned and decontaminated.
9 Certification Testing 9.1 Classification of the Safety Cabinet For application in the USA, the unit has been rated as a Class II safety cabinet, Type A2, in accordance with NSF/ANSI 49. For operation as a unit of said classifications, a certification test and repeat test in accordance with NSF/ANSI 49, Annex F should be completed at the time of installation, and at least annually thereafter.
Note: Service with costs: Unless certification was expressly called for in the specification, quotes and/or purchase order, the cost for this on-site testing is to be paid for by the customer. 9.3 Test Equipments For conducting these tests, Thermo Scientific recommends testing equipment of the manufacturers listed below. Table 18. Thermo Recommended Testing Equipment Manufacturers Testing Equipments...
9.4 Testing Information Inflow Velocity – DIM Method (Primary) Description: • Inflow velocity using the direct inflow measurement method (DIM). Equipment: • Shortridge ADM-870, or equivalent • Flow hood Series 8400, or equivalent Method: 1. Move the front window to the work position (25.4 cm / 10" or 20.3 cm / 8", depending on model). 2.
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Inflow Velocity - Constricted Window Method (Secondary) Description: • Inflow velocity measured over several points through a constricted window. Equipment: • Thermal Anemometer, or equivalent (hotwire) • Anemometer Probe Holder, P/N - 1911325 Method: 1. Lower the front window to a height of 3" (7.6 cm). 2.
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Refer to the data plate section on the GUI. Figure 60. Constricted Window Method Downflow Velocity – Uniform Cabinet Description: • Measures the velocity of air flow onto the work surface through the supply filter. Equipment: • Thermal Anemometer, or equivalent (hotwire) •...
9.5 HEPA Filter Leak Test Description: • Determines the integrity of the down flow and exhaust filters. Equipment: • Aerosol Photometer, or equivalent. • Aerosol Generator, or equivalent. Filters that can be accessed and scanned. Method: 1. Allow the cabinet to operate for approximately 20 minutes. 2.
Equipment: • Note: To test the function of fan interlocking alarm you need to access to the Field Certification screen. This function is reserved to Thermo Scientific Field Service and requires a special passcode. Acceptance: • Within 15 seconds audible and visual airflow alarms will signal. The cabinet supply fan will be de-energized.
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• Flow hood series 8400 or equivalent Note: To test the function of Airflow Alarms you need to access to the Field Certification screen. This function is reserved to Thermo Scientific Field Service and requires a special passcode. Acceptance: •...
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Method: • Introduce a smoke source into the intake on the side of the Thermo Scientific thimble connection. If the external exhaust flow is greater than the cabinet exhaust (as required), smoke will be drawn in. This demonstrates containment. •...
10 Warranty Information THERMO FISHER SCIENTIFIC LAMINAR FLOW EQUIPMENT WARRANTY USA The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner.
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