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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex™ product. Shodex PROTEIN LW-403 4D series is a high performance
silica-based gel filtration chromatography column. Since the pore size is specifically controlled, the column is
suitable for analyzing proteins with a molecular weight of several hundred of thousand.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6989403
PROTEIN LW-403 4D
F6700134
PROTEIN LS-G 4J
Base Material
: Spherical porous particles of silica modified with hydrophilic group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
Operation Manual
Shodex
PROTEIN LW-403 4D
Column Size
Particle
(mm)
Size
(μm)
I.D.
Length
4.6
150
1.9
4.6
20
1.9
SHOWA DENKO K.K. (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 11,000
10,000 - 700,000
(Guard Column)
*1
Reference value only / Measured with protein
ver.21111E
Exclusion Limit
*1
(1,000,000)
-
-
*2
Estimated value
*1
*2
1
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Summary of Contents for Shodex PROTEIN LW-403 4D
- Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex™ product. Shodex PROTEIN LW-403 4D series is a high performance silica-based gel filtration chromatography column. Since the pore size is specifically controlled, the column is suitable for analyzing proteins with a molecular weight of several hundred of thousand.
- Page 2 Product Name (MPa)(Per Column) Range Range (°C) Recommended Maximum PROTEIN LW-403 4D 0.35 3.0 - 7.5 4 - 45 PROTEIN LS-G 4J Usable solvents are listed below. (1) The standard eluent is a buffer with a salt. Buffers such as phosphate, acetate, and Tris-HCl can be used.
- Page 3 7. Column Usage Procedure PROTEIN LW-403 4D is a semi-micro column. To achieve its best performance, please use it with a semi-micro type system. 7.1 HPLC System Preparation Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used.
- Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
- Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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