Shodex OHpak LB-800 Series Operation Manual

（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex™ product. Shodex OHpak LB-800 series is a size exclusion
chromatography column used with aqueous solvents. The column series is suitable for the separations of
various water-soluble high molecular weight polymers, proteins, and/or oligomers, and molecular weight
distribution analysis of high molecular weight polymers. Successfully reduced baseline noise in the light
scattering (LS) detector by suppressing the column bleeding as much as possible made it to provide accurate
molecular weight measurements. The columns with different exclusion limits are available. Please select
columns with target molecular weight ranges (see below) that meet the molecular weight range of your samples.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6429206 OHpak LB-802.5
F6429201 OHpak LB-803
F6429204 OHpak LB-804
F6429203 OHpak LB-805
F6429205 OHpak LB-806
F6429202 OHpak LB-806M
F6709434 OHpak LB-G 6B
LB-806M is a mixed-gel column, suitable for the analysis of wide molecular weight ranges.
Operation Manual

Shodex OHpak
Column Size
Particle
(mm)
Size
I.D. Length (μm)
8.0 300 6
8.0 300 6
8.0 300 10
8.0 300 13
8.0 300 13
8.0 300 13
6.0 50 13
SHOWA DENKO K.K. (https://www.shodex.com/)

LB-800 series
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 16,000
500 - 10,000
≥ 16,000
1,000 - 100,000
≥ 16,000
5,000 - 400,000
≥ 12,000
100,000 - 1,000,000
≥ 12,000
100,000 - (20,000,000)
≥ 12,000
500 - (20,000,000)
(Guard Column)
Exclusion
*1 Limit *1
10,000
100,000
1,000,000
(4,000,000) *2
*2 (20,000,000) *2
*2 *2
(20,000,000)
- -
ver.211115E
LS Noise
(V) *3
 5.0 x 10 -5
 2.0 x 10 -5
 5.0 x 10
-5
 5.0 x 10
-5
 5.0 x 10
-5
 2.0 x 10
-5
-
1