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•
Allow the cabinet to operate until the Home Screen is
shown
BLOWER STARTING
Warm up Screen
Figure 40. Start-up
•
Wash hands and arms thoroughly with germicidal soap
•
Wear appropriate personnel protective equipment (PPE)
Wipe-Down
•
Raise the sash to its full open position (approximately
21.75" or 552 mm). Mute the alarm by pressing
[OK/MUTE]
•
Wipe down the interior surfaces of the cabinet with 70%
ethanol, or a suitable disinfectant, and allowed to dry
Loading Materials and Equipment
•
Only load the materials required for the procedure. Do not
overload the cabinet
•
Do not obstruct the front, side, or rear return air grilles
•
Large objects should not be placed close together
•
Slowly close the sash until it is in the correct operating
position
•
After loading the cabinet, wait two to three minutes to
purge airborne contaminants from the work area
Work Techniques
•
Keep all materials at least 4" (100 mm) inside from the
sash, and perform all contaminated operations as far to
the rear of the work area as possible
•
Segregate all clean and contaminated materials in the
work area
•
Arrange materials to minimize the movement of
contaminated materials into clean areas
•
Keep all discarded contaminated material to the rear of
the work area
•
Avoid moving materials or the operator's hands and arms
through the front access opening during use
•
Avoid the use of an open flame. Use disposable labware
or an electric incinerator as alternatives
•
Use proper aseptic technique
•
Avoid using techniques or procedures that disrupt the
airflow patterns of the cabinet
1500 Series Class II, Type B2 Biological Safety Cabinet
Home Screen
•
If there is a spill or splatter during use, all objects in the
cabinet should be surface decontaminated before
removal. Thoroughly disinfect the working area of the
cabinet WHILE IT IS STILL IN OPERATION, to prevent the
release of contaminants from the cabinet
Final Purging
•
Upon completion of work, the cabinet should be allowed
to operate for two to three minutes undisturbed, to purge
airborne contaminants from the work area
Unloading Materials and Equipment
•
Objects in contact with contaminated material should be
surface decontaminated before removal from the cabinet
•
All open trays or containers should be covered before
being removed from the cabinet
Wipe-Down
•
Wipe down the interior surfaces of the cabinet with a
suitable disinfectant, or 70% ethanol, and allow to dry
•
Periodically lift the work surface and wipe down the area
beneath it
•
Inspect and clean the towel catch located at the rear of
the work area, beneath the work pan
•
Dispose of rubber gloves appropriately, and have lab coat
laundered properly
•
Wash hands and arms thoroughly with germicidal soap
Shutdown
•
Lower the sash to turn off the LED light and cabinet
blower and activate the UV light if appropriate
Using the Cabinet |
25
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