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Summary of Contents for Sartorius Vivaspin 500
- Page 1 Instructions for Use Vivaspin 500 and 2 ® Centrifugal Ultrafiltration Units for General Laboratory Use 3104647-001-00...
- Page 3 Contents Contents 1 About these Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Scope .
- Page 4 Contents 7 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 7.1 Decontaminating the Product .
- Page 5 About these Instructions About these Instructions Scope These instructions are part of the product. These instructions apply to the following versions of the product: Vivaspin 500 PES Quantity Prod . no . ® 3 kDa 25 | 100 VS0191 | VS0192 5 kDa 25 | 100 VS0111 | VS0112...
- Page 6 About these Instructions Vivaspin 2 PES Quantity Prod . no . ® 3 kDa 25 | 100 VS0291 | VS0292 5 kDa 25 | 100 VS0211 | VS0212 10 kDa 25 | 100 VS0201 | VS0202 30 kDa 25 | 100 VS0221 | VS0222 50 kDa 25 | 100...
- Page 7 About these Instructions Target Groups The instructions are designed for the following target groups. The target groups must possess the knowledge listed below. Target Group Knowledge and Qualifications Operator The operator of the product is responsible for compliance with safety requirements and workplace safety regula- tions.
- Page 8 Safety Instructions Safety Instructions General Functions The product is intended for the ultrafiltration and | or diafiltration of biological and aqueous solutions. The sample solutions and volumes used must be suitable for the product. The filtration process must be carried out in a centrifuge. Macromolecules that are sufficiently larger than the nominal pore size of the membrane are retained above the membrane and progressively concentrated.
- Page 9 Safety Instructions Personnel Qualification Persons without sufficient knowledge in the safe use of the device can injure themselves and others. If a specific qualification is required for an activity: The target group is indi- cated. If no qualification is specified: The activity can be carried out by the target group “Operator”.
- Page 10 Product Description Product Description Vivaspin ® Fig. 1: Product overview (example) Pos . Description Concentrator Membrane Filtrate container Instructions for use Vivaspin 500 | 2 ®...
- Page 11 Product Description Vivaspin ® Fig. 2: Product overview (example) Pos . Description Concentrator cap Concentrator Membrane Filtrate container Instructions for use Vivaspin 500 | 2 ®...
- Page 12 Product Description Product Symbols Symbol Definition Catalogue number Do not reuse Use by Batch code Manufacturer Temperature limitation Non-sterile product Instructions for use Vivaspin 500 | 2 ®...
- Page 13 Process Preparation Process Preparation Scope of Delivery Article Product packed in a cardboard box Vivaspin 25 or 100 ® Vivaspin 25 or 100 ® Instructions for Use Unpacking Procedure NOTICE Risk of product malfunctions due to exceeding the usability! Check the usability of the product (see specification on packaging). Dispose of products for which the usability has been exceeded.
- Page 14 Operation Operation Pre-Rinsing the Product Membranes in the product may contain traces of glycerin. If this substance can interfere with the analysis of the sample: The membrane may be rinsed before filtration. Procedure Remove the concentrator cap. Use a pipette to apply a filling volume of buffer solution or deionized water into the concentrator.
- Page 15 Operation Applying the Sample It is recommended that a pipette is used to apply the sample into the product. The pipette must be compatible with the product (see Chapter "8.5.1 Pipettes," page 23). Please ensure that the molecular weight cut-off (MWCO) of the product is suitable for the size of the target molecule to be concentrated.
- Page 16 Operation Inserting the Product into the Centrifuge Procedure NOTICE Risk of product malfunctions or damage to the centrifuge! Only use the product in suitable centrifuges (see Chapter "8.5.2 Centrifuges," page 24). Insert the product into the centrifuge. If a centrifuge with fixed-angle rotor is used: Place the product into the centrifuge so that the printed volume graduations on the concentrator are facing upwards.
- Page 17 Operation Operation Vivaspin ® 5.7.1 Removing the Concentrator from the Filtrate Container Procedure To release the tube from the concentrator, pinch the tube – to press it into an oval shape – before removing it with a twisting action. Press Press here here...
- Page 18 Operation Operation 5.7.2 Reverse spin with Vivaspin ® The concentrate can be collected into the concentrator cap by reverse spinning, instead of using a pipette. Concentrator Procedure Remove filtrate container. Concen- With the concentrator cap still fitted, invert trator cap the concentrator.
- Page 19 Storage Storage Storing the Product If the product has been unpacked and membrane has been pre-rinsed: The membrane must be protected against drying out. For this purpose, the membrane must be stored in a moist and cool condition. NOTICE Risk of damage to the product due to improper storage! Comply with the storage specifications.
- Page 20 Disposal Disposal Decontaminating the Product If the product has come into contact with hazardous substances: Steps must be taken to ensure proper decontamination and declaration. The operator of the product is responsible for adhering to local government regulations on the proper decontamination and declaration for transport and disposal. Procedure If the product has come into contact with hazardous substances: Decontaminate the product.
- Page 21 Technical Specifications Technical Specifications Dimensions Vivaspin Vivaspin ® ® Unit Value Unit Value Length x Diameter 50 x 11 126 x 17 Active membrane cm² cm² surface Weight Materials Vivaspin Vivaspin ® ® Concentrator Polycarbonate Polycarbonate Filtrate container Polypropylene Polycarbonate Membrane Polyethersulfone Polyethersulfone,...
- Page 22 Technical Specifications Ambient Conditions Unit Value Storage temperature When packed °C +15 – +30 When unpacked, with membrane °C +2 – +8 kept moist Operating conditions 8.4.1 Filtration Volumes Centrifuge with Centrifuge with swing bucket fixed-angle rotor rotor (25°) Unit Value Value Vivaspin...
- Page 23 Technical Specifications 8.4.2 Centrifugation Limit Values Centrifuge Centrifuge with with swing fixed angle rotor bucket rotor Unit Value Value Vivaspin 12,000 ® Vivaspin 4,000 8,000 ® Equipment Required 8.5.1 Pipettes Pasteur pipette, variable volume or fixed volume pipette for sample appli- cation and concentrate or filtrate retrieval.
- Page 24 Technical Specifications 8.5.2 Centrifuges Centrifuge accepting conical base tubes. Required carriers Unit Value Vivaspin ® Rotor type Fixed angle Volume Diameter Minimum rotor angle ° Vivaspin ® Rotor type Swing bucket or fixed angle Volume Diameter Minimum rotor angle ° Sanitizing Methods Rinsing with 70% ethanol or with sanitizing gas mixture, e.g.
- Page 25 Typical Performance Characteristics Typical Performance Charac- teristics Vivaspin ® Time to concentrate up to 30x at 20°C Start volume 500 µL Time (min) Solute Recovery Aprotinin 0.25 mg/mL (6.5 kDa) 3 kDa PES BSA 1.0 mg/mL (66 kDa) 5 kDa PES 10 kDa PES 30 kDa PES IgG 0.25 mg/mL (160 kDa)
- Page 26 Typical Performance Characteristics Vivaspin ® Time to concentrate up to 30x at 20°C Start volume 2 mL Time (min) Solute Recovery Insulin chain A 0.1 mg/mL (2.5 kDa) 2 kDa Hydrosart ® Aprotinin 0.25 mg/mL (6.5 kDa) 3 kDa PES BSA 1.0 mg/mL (66 kDa) 5 kDa PES 5 kDa Hydrosart...
- Page 27 Chemical Compatibility 10 Chemical Compatibility Chemical, biological and aqueous solutions with appropriate compatibility for the materials of the product (2 hr contact time) Solutions Compatible pH range pH 1–9 pH 4–8 pH 1–9 Acetic Acid (25%) Acetone (10%) Acetonitrile (10%) Ammonium Hydroxide (5%) Ammonium Sulphate (saturated) Benzene (100%)
- Page 28 Chemical Compatibility Chemical, biological and aqueous solutions with appropriate compatibility for the materials of the product (2 hr contact time) Solutions Compatible pH range pH 1–9 pH 4–8 pH 1–9 Imidazole (300 mM) Isopropanol (70%) Lactic Acid (5%) Mercaptoethanol (1 M) Methanol (60%) Nitric Acid (10%) Phenol (1%)
- Page 29 Chemical Compatibility Chemical, biological and aqueous solutions with appropriate compatibility for the materials of the product (2 hr contact time) Solutions Compatible pH range pH 1–9 pH 4–8 pH 1–9 Triton ** X-100 (0.1%) ® Urea (8 M) OK = Acceptable ? = Questionable NO = Not recommended * Triton...
- Page 30 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.