Sartorius Vivaflow 50 Directions For Use Manual
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Sartorius Vivaflow 50 Directions For Use Manual

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Directions for Use
Vivaflow 50 | 50R | 200
85030-519-25
85030-519-25

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Summary of Contents for Sartorius Vivaflow 50

  • Page 1 Directions for Use Vivaflow 50 | 50R | 200 85030-519-25 85030-519-25...

  • Page 2: Table Of Contents

    Contents Vivaflow 50 Single Module Linked Modules Diafiltration|Desalting Concentration Recovery System Components Technical Specifications Performance Characteristics Ordering Information Chemical Compatibility Vivaflow 50R and Vivaflow 200 Operation of Single Module Operation of Two Modules 12 – 13 Diafiltration| Desalting Concentration Recovery...

  • Page 3: Vivaflow 50

    Filtrate vessel Sample | diafiltration reservoir (VFA006) Pink flow restrictor Diagram 1: Vivaflow 50 set up Single module 4. Pump liquid through the system to purge any air pockets. The recirculation rate 1. Set up the system as illustrated in Diagram 1.

  • Page 4: Linked Modules

    T­connector Interconnector Pressure indicator Diagram 2: Setting up multiple Vivaflow 50 cassettes Linked modules 4. The flow path of the system can be con­ 1. Set up the system as illustrated in figured to suit the membrane|sample combination. Most solutions are better Diagram 2.

  • Page 5: Diafiltration|Desalting

    Diagram 3: Buffer exchange | diafiltration with Vivaflow 50 Diafiltration| Desalting 5. As the volume in the 500 ml reservoir 1. Set up the system as illustrated in decreases, the vacuum created draws exchange solvent through the feed line Diagram 3 for linked modules. If only from the larger reservoir.

  • Page 6: Concentration

    Concentration Recovery 1. Fill the feed reservoir with sample solu­ 1. Disconnect the feed line from the lid tion. When initial volumes larger than of the 500 ml reservoir or when using 500 ml are required, place 500 ml in a different container, remove the feed line from the sample.

  • Page 7: System Components

    Masterflex easy load pump head (Prod. No. VFA012) Masterflex pump CE 230V – US 115V (Prod. No. VFP001|VFP002) Diagram 4: System componets for setting up multiple Vivaflow 50 units Table 1: Technical Specifications Dimensions Overall L | H| W 107|84|25 mm Channel W| H 15 mm| 0.3 mm...

  • Page 8: Performance Characteristics

    Materials of Construction Main housing Polycarbonate Flow channel TPX (PMP) Membrane support TPX (PMP) Seals and O rings Silicone Pressure indicator Polypropylene, SS Spring, Flow restrictor Polypropylene Fittings Nylon Tubing PVC (medical grade) Table 2: Performance characteristics Time to concentrate up to 20x (min.) at 3 bar inlet pressure, 20°C Single Three...

  • Page 9: Ordering Information

    Ordering Information Vivaflow 50 include filtrate tube, size 16 peristaltic tubing, Pack Size Prod. No. flow restrictor and fittings and 1 + Series Interconnector 3,000 MWCO (PES) VF05P9 5,000 MWCO (PES) VF05P1 10,000 MWCO (PES) VF05P0 30,000 MWCO (PES) VF05P2...

  • Page 10: Chemical Compatibility

    Table 3: Chemical Compatibility for the Vivaflow 50 Solution Solution Peracetic acid (0.2%) Acetic acid (25%) Phenol (1%) Acetone Phosphate Buffer (1 M) Ammonium hydroxide (5%) Pyridine Ammonium sulfate sat. DMEM RPMI­1640 Sodium azide Ethanol (70%) Ethyl acetate Sodium deoxycholate (5%)

  • Page 11: Vivaflow 50R And Vivaflow 200

    Vivaflow 50R and Vivaflow 200 Diagram 5: Vivaflow 50R and Vivaflow 200 set up Operation Single Vivaflow 50R or 4. Pump liquid through the system to purge Vivaflow 200 Modules any air pockets. The recirculation rate should be 200–400 ml/min, and suit­ Assembly and rinsing single module able flow should exit the filtrate line.

  • Page 12: Operation Of Two Modules

    Diagram 6: Setting up two Vivaflow 50R units Operating two Vivaflow 50R or 2. Vivaflow 50R: two Vivaflow 200 Modules Use two T­connectors (VFA030) to connect to the inlets of both Vivaflow Assembly and rinsing two modules 50R modules. 1. Set up the Vivaflow 50R system as illus­ Vivaflow 200: trated in Diagram 6 and the Vivaflow 200 Use the Y­connector, (VFA005)
  • Page 13 Diagram 7: Setting up two Vivaflow 200 units 3. Note the positioning of a flow restrictor 6. Allow 800 ml to pass into the filtrate on the return line from each Vivaflow 200 vessel. Check for any leakage at tubing module.

  • Page 14: Diafiltration| Desalting

    Concentration 1. Fill the feed reservoir with sample solution. 2. Pump liquid through the system. The recircu lation rate should be 200– 400 ml/min, (500–900 ml/min for two modules), and suitable flow should exit the filtrate line. If used, the pressure indi­ cator should read approximately 2.5 bar.

  • Page 15: Technical Specifications

    Table 4: Technical specifications Vivaflow 50R and Vivaflow 200 Dimensions Vivaflow 50 R Vivaflow 200 Overall L | H |W 100 | 100 | 24 mm 126 |138|38 mm Channel W| H 7.5 | 0.4 mm 10 mm| 0.4 mm...

  • Page 16: Cleaning

    Cleaning and storage information only for Vivaflow 50R and Vivaflow 200 Cleaning Storage Vivaflow 50R and 200 modules may be To store Vivaflow 50R and Vivaflow 200 used several times if cleaning and storage after cleaning, fill module with deionised instructions are followed.

  • Page 17: Performance Characteristics

    Table 5: Performance characteristics Vivaflow 50R Time to concentrate up to 20x (min) at 3.0 bar inlet | 2.5 bar outlet pressure, 20°C start volume Average flux Recovery % 250 ml ml/min Direct 25 ml rinse Lysozyme 0.25 mg/ml (14,000 MW) 5,000 MWCO Hydrosart 10,000 MWCO Hydrosart 10.3...

  • Page 18: Performance Characteristics

    Table 6: Performance characteristics Vivaflow 200 Time to concentrate up to 20x (min.) at 3 bar inlet pressure, 20°C start volume Average flux Recovery % 1 litre ml/min Direct 25 ml rinse Lysozyme 0.25 mg/ml (14,000 MW) 2,000 MWCO Hydrosart >...

  • Page 19: Ordering Information

    Ordering Information Vivaflow 50R modules include pressure indicator, flow restrictor Qty per Prod. no. and size 16 pvc peristaltic tubing and fittings 5,000 MWCO Hydrosart VF05H1 10,000 MWCO Hydrosart VF05H0 30,000 MWCO Hydrosart VF05H2 100,000 MWCO Hydrosart VF05H4 Vivaflow 200 modules include pressure indicator, flow restrictor Qty per Prod.
  • Page 20 Vivaflow 50R | 200 accessories Masterflex economy drive variable speed peristaltic pump (230V) VFP001 Masterflex economy drive variable speed peristaltic pump (115V) VFP002 500 ml sample and |or diafiltration reservoir VFA006 Masterflex easy load pump head – size 16 VFA012 Masterflex easy load pump head –...

  • Page 21: Chemical Compatibility

    Table 7: Chemical Compatibility for Vivaflow 50R and Vivaflow 200 Solution Solution Peracetic acid (0.2%) Acetic acid (25%) Phenol (1%) Acetone Phosphate Buffer (1 M) Ammonium hydroxide (5%) Pyridine Ammonium sulfate sat. DMEM RPMI­1640 Sodium azide Ethanol (70%) Ethyl acetate Sodium deoxycholate (5%) Sodium hydroxide (1 M) Formaldehyde (30%)
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  • Page 24 Sartorius Stedim Lab Ltd Sperry Way Stonehouse Park Gloucestershire GL10 3UT, UK www.sartorius.com Copyright by Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany. All rights reserved. No part of this publication may be reprinted or translated in any form or by any means...

This manual is also suitable for:

Vivaflow 50rVivaflow 200

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