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Summary of Contents for Sartorius Vivacon 500
- Page 1 Technical data and operating instructions ® Vivacon For in vitro use only 85034-538-74 85034-538-74...
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Page 2: Operation
® Vivacon 500 µl – Introduction Operation Storage conditions | shelf life 1. When working with DNA samples, select ® Vivacon ultrafiltration spin columns a molecular weight cut off (MWCO) which should be stored at room temperature. retains the fragment size of double The devices should be used before the stranded DNA (ds DNA) as shown in expiry date printed on the box. -
Page 3: Technical Specifications
Technical Specifications Desalting | Buffer Exchange Table 1: Technical Specifications 1. Concentrate sample to desired level. Concentrator capacity 2. Empty filtrate container. Fixed angle rotor 0.5 ml 3. Refill concentrator with an appropriate Dimensions solvent. Total length 45 mm (Concentration) 4. -
Page 4: Usage Tips
Usage Tips 1. Flow Rate 5. Retention and Recovery Filtration rate is affected by several parame- ® The membranes used in Vivacon ters, including MWCO, porosity, sample con- characterized by a molecular weight cut centration, viscosity, centrifugal force and off (MWCO). For proteins, it corresponds temperature. -
Page 5: Performance Characteristics
Performance Characteristics ® Table 3: Performance Characteristics Vivacon 500 for DNA Start volume 0.5 ml, sample concentration 50 ng/ml Sample size (bp) Time to concentrate up Concentrate g-force (xg) to 30x [min.] at 20°C recovery % 2,000 MWCO 60 min 7,500 10,000 MWCO 25 min... - Page 6 Table 6: Chemical Compatibility (2hr contact time) Hydrosart ® Cellulose Acetate Compatible pH range pH 1-9 pH 4-8 Acetic Acid (25.0%) Acetone (10.0%) Acetonitrile (10.0%) Ammonium Hydroxide (5.0%) Benzene (100%) Chloroform (1%) Dimethyl Formamide (10.0%) Dimethyl Sulfoxide (5.0%) EDTA (1.0 M) Ethanol (70.0%) Ethyl Acetate (100%) Formaldehyde (30%)
- Page 7 – DNA recovery is lower than expected If the DNA sample contains a high salt concentration, dilute the sample. Run the device at the recommended g-force. ® – Can proteins be concentrated with Vivacon ® Proteins can be concentrated with Vivacon , using the guidelines on page 5 to choose the ®...
- Page 8 Sartorius Stedim Lab Ltd Sperry Way Stonehouse Park Gloucestershire GL10 3UT, UK www.sartorius.com Copyright by Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany. All rights reserved. No part of this publication may be reprinted or translated in any form or by any means...
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