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Summary of Contents for Sartorius Vivaspin Filtrate
- Page 1 Instructions for Use Vivaspin Filtrate ® Centrifugal Ultrafiltration Devices for General Laboratory Use 3104669-001-00...
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Page 3: Table Of Contents
Contents Contents 1 About these Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Scope . - Page 4 Contents 7 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Decontaminating the Product .
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Page 5: About These Instructions
About these Instructions About these Instructions Scope These instructions are part of the product. These instructions apply to the fol- lowing versions of the product: Vivaspin Filtrate Quantity Prod . no . ® 5 kDa CTA 13229----------E 10 kDa CTA 13239----------E 20 kDa CTA 13249----------E... -
Page 6: Symbols Used
About these Instructions Symbols Used 1.3.1 Warnings in Operation Descriptions NOTICE Denotes a hazard that may result in property damage if it is not avoided. 1.3.2 Other Symbols Required action: Describes actions that must be carried out. The actions in the sequence must be carried out in succession. Result: Describes the result of the actions carried out. -
Page 7: Safety Instructions
Safety Instructions Safety Instructions General Functions The product is intended for the ultrafiltration of biological and aqueous solutions with small sample volumes, e.g. for protein removal. The filtration process must be carried out in a centrifuge. For this purpose, the sample solution must be put into the product and the product must be in- serted into a centrifuge. -
Page 8: Personnel Qualification
Safety Instructions Personnel Qualification Persons without sufficient knowledge in the safe use of the device can injure themselves and others. If a specific qualification is required for an activity: The target group is indicat- ed. If no qualification is specified: The activity can be carried out by the target group "operator". -
Page 9: Product Description
Product Description Product Description Product Overview Fig. 1: Product overview (example) Pos . Description Dust cap Centrifuge tube Filtrate vessel with membrane Instructions for Use Vivaspin Filtrate ®... -
Page 10: Product Symbols
Product Description Product Symbols Symbol Description Quantity Catalogue number Batch number Use by Non-sterile product Do not re-use Consult instructions for use Temperature limits Instructions for Use Vivaspin Filtrate ®... -
Page 11: Process Preparation
Process Preparation Process Preparation Scope of Delivery Item Quantity Product, packed in a cardboard box, according to packaging unit Instructions for Use Unpacking Procedure NOTICE Risk of product malfunctions due to exceeding the usability! Check the usability of the product (see specification on packaging). Dis- pose of products for which the usability has been exceeded. -
Page 12: Operation
Operation Operation Pre-Rinsing the Product The membrane in the product may contain traces of glycerin. If this substance can interfere with the analysis of the sample: Rinse the membrane before filtration. Procedure Remove the dust cap. Slide out the filtrate vessel and place it with the open end downwards. Do not touch the membrane. -
Page 13: Sanitizing The Product
Operation Sanitizing the Product The product can be sanitized before use. The sanitizing method must be suit- able for the product (see Chapter “8.6 Sanitizing Methods“, page 20). Procedure Remove the dust cap. Sanitize the product using the desired sanitizing method. Empty the product. -
Page 14: Performing Filtration
Operation Procedure Check whether the MWCO of the product is suitable for the application. Remove and discard the dust cap. Slide out the filtrate vessel and place it with the open end downwards. Do not touch the membrane. Apply the sample into the centrifuge tube using a pipette. Comply with the maximum filling volume. -
Page 15: Removing The Sample
Operation 5.3.3 Removing the Sample Procedure If the filtration or concentration is complete: Remove the product from the centrifuge. NOTICE Risk of reduced sample volume due to diffusion. Remove the sample from the filtrate vessel with a pipette as soon as possible after performing filtration. -
Page 16: Storage
Storage Storage Storing the Product If the product has been unpacked and the membrane has been pre-rinsed: The membrane must be protected against drying out. For this purpose, the membrane must be stored in a moist and cool condition. NOTICE Risk of damage to the product due to improper storage! Comply with the storage specifications. -
Page 17: Disposal
Disposal Disposal Decontaminating the Product If the product has come into contact with hazardous substances: Steps must be taken to ensure proper decontamination and declaration. The operator of the product is responsible for adhering to local government regulations on the proper decontamination and declaration for transport and disposal. Procedure If the product has come into contact with hazardous substances: Decontaminate the product. -
Page 18: Technical Specifications
Technical Specifications Technical Specifications Dimensions Unit Value Dimensions Length × Diameter 93 × 14 Active membrane surface 0.79 Weight Materials Materials Filtrate vessel Styrene Acrylonitrile (SAN) Centrifuge tube Polystyrene (PS) Dust cap Polyethylene (PE) Membrane Cellulose Triacetate (CTA) or Polyethersulfone (PES) Instructions for Use Vivaspin Filtrate ®... -
Page 19: Ambient Conditions
Technical Specifications Ambient Conditions Unit Value Storage temperature When packed °C +15 – +30 When unpacked, with membrane kept °C +2 – +8 moist Equipment Required 8.4.1 Centrifuges Unit Value Swing bucket rotor or fixed angle rotor Minimum rotor angle for fixed angle rotor 25°... -
Page 20: Operating Conditions
Technical Specifications Operating Conditions Unit Value Filtration Volumes Filling volume, minimum Filling volume, maximum Membrane hold-up volume, minimum µL <5 Dead stop volume µL Relative Centrifugal Force, maximum Centrifuge with swing bucket rotor 2,500 Centrifuge with fixed angle rotor 2,000 The dead stop volume may vary depending on the type and concentration of the sample, operating temperature and | or centrifuge rotor Sanitizing Methods... -
Page 21: Typical Performance Characteristics
Technical Specifications Typical Performance Characteristics 2.5 mL start volume in Time to filter Time to filter Passage of centrifuge at 2,000 g 50 % of 90 % of sample species sample volume sample volume BSA 1.0 mg/ml (66 kDa) 5 kDa MWCO 300 min 10 kDa MWCO 35 min 80 min... - Page 22 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.
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