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Technical data and operating instructions
®
Vivaspin
Vivaspin
®
500 and 2 10K devices for in vitro diagnostic use
®
Vivaspin
500 and 2 3K, 5K, 30K, 50K, 100K, 300K, 1000K and 0.2 µm
devices for research use only; not for use in diagnostic procedures
For French, Italian, and Spanish manuals, please go to
www.sartorius.com/en/product-family/product-family-detail/m-centrisart-i/
85030-511-58
500 µl and 2 ml
85030-511-58
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Summary of Contents for Sartorius Vivaspin 500
- Page 1 500 and 2 10K devices for in vitro diagnostic use ® Vivaspin 500 and 2 3K, 5K, 30K, 50K, 100K, 300K, 1000K and 0.2 µm devices for research use only; not for use in diagnostic procedures For French, Italian, and Spanish manuals, please go to www.sartorius.com/en/product-family/product-family-detail/m-centrisart-i/ 85030-511-58 85030-511-58...
- Page 2 Vivaspin ® 500 µl and 2 ml – Introduction Storage conditions| shelf life ® Vivaspin ultrafiltration spin columns The Vivaspin ® 500 & 2 product line includes should be stored at 15 – 30°C. The devices 9 different cutoffs (Molecular Weight Cutoff, should be used before the expiry date MWCO): printed on the box.
- Page 3 5 k, 10k, and 30 k molecular weight cutoffs. Please note that membrane behaviour largely depends on the specific characteristics of the solution being processed. Sartorius Stedim Biotech recommends that users experiment with alternative membranes in seeking to optimise their process performance.
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Page 4: Operation
Operation Removing the Vivaspin ® 2 body from 1. Select the most appropriate membrane the filtrate tube for your sample. For maximum recovery The sleeve (seen from the end) is oval in select a MWCO at least 50% smaller than cross section. - Page 5 Reverse spin with Vivaspin ® Depending on user preference and need Vivaspin ® 2 Reverse Spin for sample storage, the concentrate can be reverse spun into the concentrate recovery Position with printed cap (when fixed angle rotors are used, angle window upwards|outwards concentrator so that the printed window faces upwards| outwards).
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Page 6: Technical Specifications
Technical Specifications Table 1: Technical specifications ® ® Vivaspin Vivaspin Concentrator capacity Swing bucket rotor do not use 3 ml Fixed angle rotor 500 µl 2 ml Dimensions Total length 50 mm 126 mm Width 11 mm 17 mm Active membrane area 0.5 cm 1.2 cm Hold-up volume,... - Page 7 Usage Tips 3. Sanitization of Polyethersulfone 1. Flow Rate Membranes Filtration rate is affected by several parame- ® Vivaspin devices should not be autoclaved ters, including MWCO, porosity, sample con- as high temperatures will substantially centration, viscosity, centrifugal force and increase membrane MWCO.
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Page 8: Performance Characteristics
Performance Characteristics Table 3a: Typical Performance Characteristics Vivaspin ® Time to concentrate up Concentrate to 30x [min.] at 20°C recovery % Start volume 500 µl 500 µl Aprotinin 0.25 mg/ml (6,500 MW) 3,000 MWCO PES BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 10,000 MWCO PES 30,000 MWCO PES... - Page 9 Table 3b: Typical Performance Characteristics Vivaspin ® Time to concentrate up Concentrate to 30x [min.] at 20°C recovery % Start volume 2 ml 2 ml Insulin chain A 0.1 mg/ml (2,535 MW) ® 2,000 MWCO Hydrosart Aprotinin 0.25 mg/ml (6,500 MW) 3,000 MWCO PES BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES...
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Page 10: Chemical Compatibility
Chemical Compatibility Table 4: Chemical Compatibility (2hr contact time) Solutions Compatible pH range pH 1–9 pH 4–8 pH 1–9 Acetic Acid (25.0%) Acetone (10.0%) Acetonitrile (10.0%) Ammonium Hydroxide (5.0%) Ammonium Sulphate (saturated) Benzene (100%) n-Butanol (70%) Chloroform (1.0%) Dimethyl Formamide (10.0%) Dimethyl Sulfoxide (5.0%) Ethanol (70.0%) Ethyl Acetate (100%) - Page 11 Solutions Compatible pH range pH 1–9 pH 4–8 pH 1–9 Phenol (1.0%) Phosphate Buffer (1.0 M) Polyethylene Glycol (10%) Pyridine (100%) Sodium Carbonate (20%) Sodium Deoxycholate (5.0%) Sodium Dodecylsulfate (0.1 M) Sodium Hydroxide (2.5 M) Sodium Hypochlorite (200 ppm) Sodium Nitrate (1.0%) Sulfamic Acid (5.0%) Tetrahydrofuran (5.0%) Toluene (1.0%)
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Page 12: Ordering Information
Ordering Information Ordering Tips – Choose a membrane pore size at least 50% smaller than the size of the molecule to be retained. – Usually choose Polyethersulfone membranes for fastest concentrations. – Usually choose Cellulose Triacetate for Protein Removal| Ultrafiltrate recovery. ®... - Page 13 Vivaspin ® 2 Polyethersulfone Qty per box Prod. no. 3,000 MWCO VS0291 3,000 MWCO VS0292 5,000 MWCO VS0211 5,000 MWCO VS0212 10,000 MWCO VS0201 10,000 MWCO VS0202 30,000 MWCO VS0221 30,000 MWCO VS0222 50,000 MWCO VS0231 50,000 MWCO VS0232 100,000 MWCO VS0241 100,000 MWCO VS0242...
- Page 14 Vivaspin ® 2 Cellulose triacetate Qty per box Prod. no. 5,000 MWCO VS02U1 5,000 MWCO VS02U2 10,000 MWCO VS02V1 10,000 MWCO VS02V2 20,000 MWCO VS02X1 20,000 MWCO VS02X2 ® ® Vivaspin 2 Hydrosart Qty per box Prod. no. 2,000 MWCO VS02H91 2,000 MWCO VS02H92...
- Page 15 Status: September 2018, Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany Specifications subject to change without notice. Copyright Sartorius Stedim Biotech GmbH. Printed in the EU on paper bleached without chlorine. Publication No.: SLU6093-e180910 Ver. 09 | 2018...
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