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500 and 2 10K devices for in vitro diagnostic use ® Vivaspin 500 and 2 3K, 5K, 30K, 50K, 100K, 300K, 1000K and 0.2 µm devices for research use only; not for use in diagnostic procedures For French, Italian, and Spanish manuals, please go to www.sartorius.com/en/product-family/product-family-detail/m-centrisart-i/ 85030-511-58 85030-511-58...
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Vivaspin ® 500 µl and 2 ml – Introduction Storage conditions| shelf life ® Vivaspin ultrafiltration spin columns The Vivaspin ® 500 & 2 product line includes should be stored at 15 – 30°C. The devices 9 different cutoffs (Molecular Weight Cutoff, should be used before the expiry date MWCO): printed on the box.
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5 k, 10k, and 30 k molecular weight cutoffs. Please note that membrane behaviour largely depends on the specific characteristics of the solution being processed. Sartorius Stedim Biotech recommends that users experiment with alternative membranes in seeking to optimise their process performance.
Operation Removing the Vivaspin ® 2 body from 1. Select the most appropriate membrane the filtrate tube for your sample. For maximum recovery The sleeve (seen from the end) is oval in select a MWCO at least 50% smaller than cross section.
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Reverse spin with Vivaspin ® Depending on user preference and need Vivaspin ® 2 Reverse Spin for sample storage, the concentrate can be reverse spun into the concentrate recovery Position with printed cap (when fixed angle rotors are used, angle window upwards|outwards concentrator so that the printed window faces upwards| outwards).
Technical Specifications Table 1: Technical specifications ® ® Vivaspin Vivaspin Concentrator capacity Swing bucket rotor do not use 3 ml Fixed angle rotor 500 µl 2 ml Dimensions Total length 50 mm 126 mm Width 11 mm 17 mm Active membrane area 0.5 cm 1.2 cm Hold-up volume,...
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Usage Tips 3. Sanitization of Polyethersulfone 1. Flow Rate Membranes Filtration rate is affected by several parame- ® Vivaspin devices should not be autoclaved ters, including MWCO, porosity, sample con- as high temperatures will substantially centration, viscosity, centrifugal force and increase membrane MWCO.
Ordering Information Ordering Tips – Choose a membrane pore size at least 50% smaller than the size of the molecule to be retained. – Usually choose Polyethersulfone membranes for fastest concentrations. – Usually choose Cellulose Triacetate for Protein Removal| Ultrafiltrate recovery. ®...
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Status: September 2018, Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany Specifications subject to change without notice. Copyright Sartorius Stedim Biotech GmbH. Printed in the EU on paper bleached without chlorine. Publication No.: SLU6093-e180910 Ver. 09 | 2018...
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