Sartorius Picus User Manual

Sartorius Picus User Manual

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Picus® & Picus® NxT Electronic Pipette
User Manual

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Table of Contents
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Summary of Contents for Sartorius Picus

  • Page 1 Picus® & Picus® NxT Electronic Pipette User Manual...
  • Page 2: Table Of Contents

    Table of Contents 1. Introduction 1.1. Intended use 1.2. Product Overview 1.2.1. Single and Multichannel Pipettes 1.2.2. Display 1.2.3. Softkey functions 1.2.4. Optifit Tips and Safetyspace Filter Tips 1.3. Content of delivery package 2. Getting Started 2.1. Charging 2.2. Power Up 3.
  • Page 3: Introduction

    Optiload tip-loading feature together with the electronic tip ejection function. To improve safety by reducing the risk of contamination, replaceable Safe-Cone Filters can be used in all Picus®/Picus® NxT models greater than 10 µl. The fully electronic operation, the unique DC-motor, electronic brake and piston control system, guarantee excellent accuracy and precision.
  • Page 4: Intended Use

    Intended Use Picus®/Picus® NxT pipette is intended to be used in liquid handling applications for dispensing liquids in the volume range of 0,2 µl to 10 ml. It is recommended to use Optifit Tips or SafetySpace Filter Tips to ensure the best compatibility and performance with Sartorius pipettes.
  • Page 5: Display

    −Right Softkey (ADV, OK, CLEAR) 1.2.4 Optifit Tips and SafetySpace Filter Tips It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes. This ensures the best possible compatibility and thus best possible accuracy and precision, as these tips are designed for the pipette in question.
  • Page 6: Content Of Delivery Package

    Picus®/Picus® NxT is delivered ready for use in a package containing: – Picus®/Picus® NxT electronic pipette – USB-charger – Sartorius tip rack (10x96 tips) with up to 1000 µl single-channel models/ 1200 µl multichannel models – One tip with 5 ml and 10 ml single-channel models –...
  • Page 7: Getting Started

    2. Getting Started Please read this manual before using Picus®/Picus® NxT electronic pipette. 1. Picus®/Picus® NxT is delivered ready for use. 2. Insert a replaceable Safe-Cone Filter into the tip-cone before use, to prevent contamination of the pipette. 3. Charge the pipette before first use for one (1) hour.
  • Page 8: Operation

    3. Operation 3.1 Principles of Operation Pipetting functions are controlled using the operating button, adjustment wheel and Softkeys. The Hotkey is for storing or activating a program. Operating button −Used to confirm the settings (alternative to the Softkey for OK) and initiate the piston movement for aspiration, dispensing and repeated blow-out.
  • Page 9: Pipetting Modes

    3.2 Pipetting Modes The Picus® /Picus® NxT electronic pipette has eight (8) main pipetting modes. The Picus® NxT has seven (7) additional modes while the Picus® has six (6) additional modes. Pipetting Modes Additional Modes (ADV) - available in all Picus® &...
  • Page 10: Pipetting (Forward Pipetting)

    Additional modes Counter, Mixing and Tracker can be used in conjunction with the Pipetting mode. Additionally, in Picus® NxT, Repeated Blow-out is available for use with the Pipetting mode. Repeated Blow-out helps to remove the residual liquid from the tip, if the liquid is such that normal dispensing does not completely empty the tip.
  • Page 11: Reverse Pipetting

    2. Turn the adjustment wheel to set the desired value. 3. Confirm the setting by pressing a. the operating button or the right Softkey for OK, and simultaneously exit the editing mode b. the middle Softkey for NEXT, and move on to edit the next, highlighted, parameter.
  • Page 12: Manual Pipetting

    To execute the selected program: 1. Press the operating button to aspirate the liquid (selected volume + excess) in. 2. Press the operating button again to discard the prime excess. 3. Press the operating button repeatedly untill all aliquots have been dispensed.
  • Page 13: Diluting

    6. Eject the tip by pressing the electronic tip ejector button. Additional modes: Mixing, and repeated Blow-out with Picus® NxT, can be used in conjunction with Diluting. Mixing mixes the liquids manually or automatically. The mixing volume can be adjusted according to the pipette’s maximum volume.
  • Page 14: Sequential Dispensing

    5. Eject the tip by pressing the electronic tip ejector button. Additional modes Excess Adjustment, and Repeated Blow-out with Picus® NxT, can be used in conjunction with Sequential Dispensing. Excess Adjustment can be used to set the excess volume. Repeated Blow-out helps to remove the residual liquid from the tip, if the liquid is such that the normal dispensing does not completely empty the tip.
  • Page 15: Titration

    3. Eject the tip by pressing the electronic tip ejector button. Additional mode Repeated Blow-out, available with Picus® NxT, can be used in conjunction with Multi-Aspiration mode, if the liquid is such that the normal dispensing does not completely empty the tip.
  • Page 16: Additional Modes

    Tracker Tracker is a helpful feature for guiding the user to dispense into correct microplate wells. It is a unique feature only available with Picus® and Picus® NxT pipettes and can be used with the following main modes: Pipetting, Reverse Pipetting and Multi-Dispensing.
  • Page 17 12-channel pipettes: 1. Select the microplate size (96 or 384 well plate). Pipetting is possible in rows only. 2. Press the operating button or the right Softkey for OK to activate the Tracker. 3. The wells, into which to pipette, are shown on the display: For 96 well plates, rows are shown as A, B, C….
  • Page 18 Excess Adjustment Excess Adjustment can be used to set the excess volume with Reverse Pipetting, Multi-Dispensing and Sequential Dispensing modes, where excess volume is used. The excess volume varies according to the volume range of the pipette. To set the excess volume: 1.
  • Page 19: Saving Pipetting Programs To Memory

    3.3 Saving Pipetting Programs to Memory All Picus® and Picus® NxT models allow the saving of 10 programs into the memory of the pipette. To store the program in the memory: 1. Choose the pipetting mode and set the parameters as guided in the previous chapter, then press the Hotkey (H) to see the list of memory locations.
  • Page 20 2 or 3 point adjustment, if performance over the whole 10 % 50 % 100 % range is required. 1-point adjustment in Picus Target Volume The adjustment options are: In 2-point −1-point adjustment: adjustment in one point only, the user can choose the...
  • Page 21: Sound

    10. Then, the pipette asks if you want to save the adjustment data. Confirm the saving of the data by pressing the operating button or the right Softkey for YES. If you do not wish to save the data, press the left Softkey for NO.
  • Page 22: User Id

    To change the colour of the display backlight: 1. Select MENU by pressing the left Softkey. 2. Use the adjustment wheel to select Setup. Confirm the selection by pressing the operating button or the Softkey for OK. 3. Use the adjustment wheel again to select Backlight. Confirm the selection by pressing the operating button or the Softkey for OK.
  • Page 23: Glp Info

    3.4.5 GLP Info GLP (Good Laboratory Practice) Info feature allows the storage of dates to indicate when the pipette was last serviced/calibrated and when the next service/calibration is due. To change the dates: 1. Select MENU by pressing the left Softkey. 2.
  • Page 24: Languages

    The saved programs can optionally be protected with a password from Chinese Russian unauthorized editing in Picus® NxT pipettes. With the Password setting, the password can be activated, deactivated and changed. To activate or deactivate the Password feature: 1. Select MENU by pressing the left Softkey.
  • Page 25: Rfid

    8. Once you are back in the Setup menu, press the left Softkey for BACK two times to return to the main display. 3.5. RFID Picus® NxT is RFID enabled to support asset management. To recall the information stored on the RFID chip, the reader and the software for RFID type HTS2048 are required.
  • Page 26: Single-Channel Pipettes

    −Never use the pipette without tip attached to the tip-cone. −Tools needed: lint-free cloth, cleaning solution, e.g. 70% ethanol. 4.1.1 Single-channel pipettes Cleaning the outer surface of all models Clean any visible dirt with mild detergent or similar and a lint-free cloth. Wipe dry.
  • Page 27: Multichannel Pipettes

    Sartorius service provider. Please contact your nearest Sartorius service provider or distributor. 4.2 Sterilizing The lower parts of Sartorius electronic pipettes can be sterilized by autoclaving, UV or by using disinfectant or decontaminant liquids such as 70% ethanol, 60% isopropanol, mild detergent or similar. Always make sure of the chemical compatibility between the materials of the pipette and the disinfectant or decontaminant liquid.
  • Page 28: Uv Sterilization

    Sartorius service provider. Performance Testing It is recommended that the performance of the Sartorius pipettes is checked regularly (e.g. every 3 months) and always after in-house maintenance. A regular testing routine should be established by users, for their pipettes,...
  • Page 29: Replacing The Battery

    (ISO 8655-1). Replacing the Battery It is recommended that the Picus®/Picus® NxT battery is replaced only by an authorised Sartorius service provider. Please contact your nearest Sartorius representative.
  • Page 30: Warranty

    5. Warranty Sartorius products are meant to be used as described in this user manual. Picus®/Picus® NxT electronic pipettes are covered by warranty for two years against defects in workmanship and materials. The battery is not covered by our warranty.
  • Page 31: Troubleshooting

    Problem Possible Cause Solution Leakage – Incompatible tip – Use original Sartorius tips – Tip is loose – Attach the tip firmly – Clean the pipette – Pipette is dirty – Replace the broken parts or send the pipette for service –...
  • Page 32: Technical Data

    Li-Polymer battery with protection circuit Capacity 3,7 V/350 mAh Charging time Approx. 1 hour Chargers, charging stands USB-charger for Picus®/Picus® NxT pipette For Indoor and Office Equipment Use Only Input voltage 100 – 240V ~ 50/60Hz, 180mA Output voltage 5V, 1A LPS...
  • Page 33: Performance Specifications

    Performance Specifications 1-channel Increment Test volume Systematic Error Random Error (µl) (µl) +/- % +/- µl +/- % +/- µl 0,2-10 µl 0,01 0,90 0,09 0,40 0,04 1,00 0,05 0,70 0,035 2,50 0,025 1,50 0,005 5–120 µl 0,10 0,40 0,48 0,15 0,18 0,60...
  • Page 34: Speed Table

    Speed Table Speed is measured in Pipetting mode with the maximum volume. Speed can be adjusted in all main pipetting modes individually for aspiration and dispensing. Speed range is from 1 (slow) to 9 (fast). Single-channel pipettes (speed in seconds) Speed 10 µl 120 µl...
  • Page 35: Ordering Information

    10. Ordering Information Picus® NxT and Picus® Picus® NxT Picus® Channels Volume Range (µl) Safe-Cone Filters Optifit Tips (µl) SafetySpace Filter Tips (µl) Order Code Order Code Standard Plus LH-745021 735021 0,2-10  721008 721018 LH-745041 735041 5-120  721007...
  • Page 36: Declaration Of Conformity

    Sartorius Biohit Liquid Handling Oy, Laippatie 1, 00880 Helsinki, Finland _______________________________________________________________________________________________ Sartorius Biohit Liquid Handling Oy, Head Office: Laippatie 1, 00880 HELSINKI, FINLAND Tel: +358 9 755 951, Fax: +358 9 755 95 200, Business ID: FI2441885-8. Internet site www.sartorius.com. Contact us: lhinfo.finland@sartorius.com...
  • Page 38 The status of the information, specifications and illustrations in this manual are subject to change. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. All trademarks are Sartorius property unless otherwise stated.

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