Related Manuals for Sartorius Vivapore 5
Summary of Contents for Sartorius Vivapore 5
- Page 1 Instructions for Use Vivapore 5 | 10 ® Solvent Absorption Ultrafiltration Units for General Laboratory Use 3163992-001-00...
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Page 3: Table Of Contents
Contents Contents 1 About these Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Scope . - Page 4 Contents 8 Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Dimensions .
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Page 5: About These Instructions
About these Instructions About these Instructions Scope These instructions are part of the product. These instructions apply to the following versions of the product: Quantity Prod . no . Vivapore ® 7.5 kDa PES VP-S005P0008--3 Vivapore ® 7.5 kDa PES VP-S010P0008--3 Target Groups The instructions are designed for the following target groups. -
Page 6: Symbols Used
About these Instructions Symbols Used 1.3.1 Warnings in Operation Descriptions NOTICE Denotes a hazard that may result in property damage if it is not avoided. 1.3.2 Other Symbols Required action: Describes actions that must be carried out. The actions in the sequence must be carried out in succession. Result: Describes the result of the actions carried out. -
Page 7: Safety Instructions
Safety Instructions Safety Instructions General functions The product is intended for the concentration and | or desalting of proteins and macromolecules from dilute solutions. The sample solutions and volumes used must be suitable for the product. The filtration process uses an absorbent cellulose pad mounted behind the ultrafiltration membrane to draw solvents and micromolecules through the membrane. -
Page 8: Personnel Qualification
Safety Instructions Personnel Qualification Persons without sufficient knowledge in the safe use of the device can injure themselves and others. If a specific qualification is required for an activity: The target group is indicat- ed. If no qualification is specified: The activity can be carried out by the target group “Operator”. -
Page 9: Product Description
Product Description Product Description Vivapore ® Fig. 1: Product overview (example) Pos . Description Membrane Dead stop pocket Instructions for Use Vivapore 5 | 10 ®... -
Page 10: Vivapore
Product Description Vivapore ® Fig. 2: Product overview (example) Pos . Description Membrane Dead stop pocket Instructions for Use Vivapore 5 | 10 ®... -
Page 11: Product Symbols
Product Description Product Symbols Symbol Definition Catalogue number Do not reuse Use by Batch code Manufacturer Temperature limitation Consult instructions for use Non-sterile product Instructions for Use Vivapore 5 | 10 ®... -
Page 12: Process Preparation
Process Preparation Process Preparation Scope of Delivery Article Product packed in a cardboard box Vivapore 5 | 10 30 | 30 ® disposable stand Instructions for Use Unpacking Procedure NOTICE Risk of product malfunctions due to exceeding the usability! Check the usability of the product (see specification on packaging). Dispose of products for which the usability has been exceeded. -
Page 13: Operation
Operation Operation Concentrating Macromolecules It is recommended that a pipette is used to apply the sample into the product. The pipette must be compatible with the product (see Chapter “8.5.1 Pi- pettes“, page 18). Please ensure that the molecular weight cut-off (MWCO) of the product is suitable for the size of the target molecule to be concentrated. -
Page 14: Desalting (Vivapore ® 10 Only)
Operation 5.1.2 Vivapore ® Procedure Check whether the MWCO of the product is suitable for the application. Insert the product into a disposable stand. If the volume to be concentrated is larger than 10 mL, insert an expansion reservoir into the aperture at the top of the product. Pipette the sample through the aperture at the top of the product. -
Page 15: Storage
Storage Storage Storing the Product NOTICE Risk of damage to the product due to improper storage! Comply with the storage specifications. Procedure If the product is packaged: Store the product in the original packaging. Store the product according to the ambient conditions (see Chapter “8.3 Ambient Conditions“, page 17). -
Page 16: Disposal
Disposal Disposal Decontaminating the Product If the product has come into contact with hazardous substances: Steps must be taken to ensure proper decontamination and declaration. The operator of the product is responsible for adhering to local government regulations on the proper decontamination and declaration for transport and disposal. Procedure If the product has come into contact with hazardous substances: Decontaminate the product. -
Page 17: Technical Specifications
Technical Specifications Technical Specifications Dimensions Vivapore Vivapore ® ® Unit Value Unit Value Length x Width 82 x 42 100 x 46 Active membrane cm² cm² surface Materials Vivapore Vivapore ® ® Concentrator Styrene acrylonitrile Styrene acrylonitrile resin resin Absorbent Cellulose Cellulose Membrane... -
Page 18: Operating Conditions
Technical Specifications Operating Conditions Vivapore Vivapore ® ® Unit Value Value Filling volume, maximum Filling volume with expansi- on reservoir, maximum Dead stop volume µL The dead stop volume may vary depending on the type and concentration of the sample, operating temperature and pH Equipment Required 8.5.1... -
Page 19: Chemical Compatibility
Technical Specifications Chemical Compatibility Chemical, biological and aqueous solutions with appropriate compatibility for the materials of the product (2 hr contact time) Examples of compatible chemical solutions Acetic Acid (25%) Phosphate Buffer pH 7 - 8 (1 M) Ammonium Sulphate (saturated) Polyethylene Glycol (10%) Butanol (70%) Sodium Carbonate (20%) -
Page 20: Typical Performance Characteristics
Technical Specifications Typical Performance Characteristics Time to concentrate at 20 .5°C Product Vivapore Vivapore ® ® Unit Start volume α-chymotrypsin ** (25 kDa) Concentrator factor 138x Time IgG** (150 kDa) Concentrator factor 179x Time *With Vivapore 10 Expansion Reservoir ® ** Proteins were concentrated from human urine specimens (pH 4.5), averaged results from devices tested;... -
Page 21: Accessories
Accessories Accessories Accessories Quantity Prod . no . Vivapore Stand for 4 units VP-AST0001-C ® Vivapore 10 Expansion Reservoir VP-ARV0010-D ® Instructions for Use Vivapore 5 | 10 ®... - Page 22 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.
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