Hitachi ChromasterUltra Rs 6270 Instruction Manual page 50

2.2 Operating Principle and Functional Description
Wash fluid 1
Wash fluid 2
Wash fluid 1
Wash fluid 2
Table 2-2 Isocratic Analysis
One Fluid Wash <standard>
(for Cut injection method,
Full loop injection method)
*1)
Sample solution ,
*1)
Mobile phase
*2)
Aqueous solvent
Table 2-3 Gradient Analysis
One Fluid Wash <standard>
(for Cut injection method,
Full loop injection method)
*1)
Sample solution ,
*2)
Aqueous solvent
*1) As a washing solution in cases where a solvent
containing a buffer solution or salt has been fed as the
mobile phase, use a mobile-phase solvent containing
no salt.
(When 20 mmol/L KH
the mobile phase, use H
If a solvent containing salt is used for washing, it causes
damages, such as the surface of the glass, the tip of the
syringe, and the inside of the wash pump.
Additionally, it is resulting in deterioration of
performance or shorter service life.
*2) Higher sample solubility solvent is used.
(Some types of samples are dissolved with 100%
organic solvent.)
NOTE:
SUS316, Vespel®, UHMWPE, PTFE, PVDF, FFPM,
ETFE, EPDM, PP, PEEK, PCTFE and FEP materials are
used for the wetted parts of the instrument. Never use
solvents that would corrode these materials.
NO.
1 Purified water
2 Methanol
3 Ethanol
4 2-propanol
5 Acetonitrile
6 Phosphoric acid
7 TFA
8 Tetrahydrofuran
Two Fluids Wash
(for All volume injection method,
Low carry over analysis)
Sample solution
(Strong wash)
Mobile phase
Two Fluid Wash
(for All volume injection method,
Low carry over analysis)
Sample solution
Gradient final solvent
(Strong wash)
Gradient initial solvent
(Weak wash)
PO :CH
2 4
O:CH
2
Name
2 - 10
*1)
*1)
(Weak wash)
*1)
*2)
*1)
CN＝50:50 is used for
3
CN＝50:50.)
3
Comments
-
-
-
-
-
Usually, 0.1 %
Usually, 0.1 %
Approx. up to 5 %