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Instruction Sheet 69-5243-134 February 12, 2010
8. Click OK to start the run.
If you selected Liquid or Solid (Pause), continue with
step 9.
If you selected None, skip steps 9 through 11 and
continue with step 12.
9. The system will equilibrate the column. During
this time, prepare the sample.
If you selected Liquid, dissolve the sample in a
❍
minimal volume of Solvent A (or other misci-
ble solvent) and draw the liquid into a
syringe. Alternatively, prepare the optional
Sample Load Pump.
If you selected Solid (Pause), prepare the sample
❍
using a solid sample cartridge. Refer to the
Help topic "Preparing Solid Samples" for
instructions.
Liquid Samples
Solid Samples
Figure 2: Pause messages
10. The CombiFlash Torrent module will pause after
performing the column equilibration (Figure 2).
It will ask you to inject the sample or load the
solid sample cartridge.
If Liquid – inject or pump the liquid into the
sample injection port. To force the sample
through the valve, follow this injection with
another 5 mL of the starting mobile phase
(chase solvent).
If Solid – load the cartridge on the
CombiFlash Torrent module. To load the car-
tridge:
Teledyne Isco, Inc.
P.O. Box 82531, Lincoln, Nebraska, 68501 USA
Toll-free: (800) 775-2965 ˘ Phone: (402) 464-0231 ˘ Fax: (402) 465-3001
E-mail: IscoService@teledyne.com
a. The solid load cartridge cap must match the
size of your selected cartridge. If it does not
match, install the correct cap now.
b. Press the lever on the side of the cap and fully
extend the plunger.
c. Slide the plunger into the cartridge until it
reaches the top frit.
d. Press the lever and push the cartridge into
the cap. Align the cartridge so that it fits fully
into the recess in the cap.
e. Rotate the cartridge
cap. For 25 gram cartridges, rotate the car-
tridge clockwise. For cartridge sizes 65 gram
and larger, rotate the cartridge counterclock-
wise.
Note
Refer to the instruction sheet titled "Adjustable Rf
Solid Load Cartridge Cap for RediSep Rf" (available at
www.isco.com) for complete instructions.
11. Click OK to begin the run.
12. Monitor the red absorbance trace.
The CombiFlash Torrent module will proceed
through the run and collect fluids in the fraction
tubes. It isolates peak fluids from non-peak flu-
ids. Tube change marks on the screen and the
tube map help you locate your compounds.
For detailed operating instructions, refer to the
on-line help.
Operation Tip: Difficult Separations — If the TLC indi-
cates a difficult separation (R
button at onset of each peak in the absorbance trace.
This inserts an isocratic hold and extends the run. The
isocratic hold can increase the resolution by delaying
the release of the next closely eluting peak. When the
absorbance of the peak is close to the baseline again,
click the Pause button to resume the linear gradient
which will help to maintain sharp peaks.
Assistance Available
Should you need assistance with these instructions,
please contact Teledyne Isco.
1
/
-turn to secure it in the
4
<0.2), click the Pause
f
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