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Related Manuals for Thermo Scientific invitrogen EVOS OSI-2
Summary of Contents for Thermo Scientific invitrogen EVOS OSI-2
- Page 1 EVOS Onstage Incubator (OSI-2) ™ USER GUIDE for use with: EVOS M5000 Imaging System ™ EVOS M7000 Imaging System ™ Catalog Number AMC2000 Publication Number MAN0027987 Revision A.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Life Technologies Corporation | 22025 20th Ave SE Ste. 100 | Bothell, Washington 98021 USA Revision history: MAN0027987 A.0 (English) Revision Date Description 6 March 2023 Initial release. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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Page 3: Table Of Contents
Contents ■ CHAPTER 1 Product information ..........5 Product description . - Page 4 Contents ■ APPENDIX B Compatible vessel holders and stage plates ....36 EVOS Onstage Incubator (OSI-2)-compatible vessel holders and stage plates ..36 ™...
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Page 5: Chapter 1 Product Information
Product information IMPORTANT! Before using this product, read and understand the information in the “Safety” appendix in this document. Product description EVOS Onstage Incubator (OSI-2) ™ The EVOS Onstage Incubator (OSI-2) is an optional accessory for the EVOS M5000 and EVOS ™... -
Page 6: Required Materials Not Provided
Chapter 1 Product information Product description • Power Cord, Type A (North America) Note: A country-specific power cord must be ordered separately in regions not using the Type A power plug. Required materials not provided • CO gas or CO /air premix for CO regulation •... -
Page 7: Incubator Components
Chapter 1 Product information Incubator components Incubator components Control unit and Environmental chamber Figure 1 EVOS Onstage Incubator (OSI-2) Control unit and Environmental chamber ™ Control unit Heated hose Hose heater connector Water reservoir door Water reservoir door release Environmental chamber Sensor data cable with 6‑pin connector EVOS Onstage Incubator (OSI-2) User Guide ™... -
Page 8: Control Unit (Back View)
Chapter 1 Product information Incubator components Control unit (back view) Figure 2 Back view of the OSI-2 Control unit showing the locations of the gas ports, input jacks, and the power switch Port 1: Air or air‑CO premix Port 2: CO Port 3: N Power switch Power input jack 6‑pin sensor data cable jack... -
Page 9: Status Led Modes
Chapter 1 Product information Incubator components Status LED modes The status indicator LED on the OSI-2 control unit identifies the status of the incubator. Figure 3 OSI-2 status indicator LED on the control unit Status indicator LED LED color LED mode Incubator status Blue Solid The OSI-2 is powered on and connected to the computer, but no functions have been enabled. -
Page 10: Enviromental Chamber
Chapter 1 Product information Incubator components Enviromental chamber The environmental chamber of the EVOS Onstage Incubator (OSI-2) consists of the incubator ™ chamber, the vessel holder/adaptor, the heated glass lid, the light shield, and the light shield cover. The environmental chamber sits on the onstage incubator master stage plate that is attached to the X-Y stage of the imaging system. - Page 11 Chapter 1 Product information Incubator components arrows _Libary. Figure 5 Bottom view of the Environmental chamber lid showing the lid contacts Environmental chamber lid contacts EVOS Onstage Incubator (OSI-2) User Guide ™...
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Page 12: Chapter 2 Methods
Methods Set up the EVOS Onstage Incubator (OSI-2) ™ IMPORTANT! Before using this product, read and understand the information in the “Safety” appendix in this document. Install the EVOS Onstage Incubator (OSI-2) ™ Assemble the Environmental chamber 1. Place the incubator chamber on the onstage incubator master plate, then secure it in place with the four 2.0‑mm hex screws. - Page 13 Chapter 2 Methods Set up the EVOS Onstage Incubator (OSI-2) ™ Install the Onstage Incubator Master Plate Note: The images in the following steps show the installation of the master plate on the EVOS M5000 ™ Imaging System X‑Y stage. However, the steps to install the master plate on the EVOS M7000 Imaging ™...
- Page 14 Chapter 2 Methods Set up the EVOS Onstage Incubator (OSI-2) ™ 2. Secure the onstage incubator master plate with the environmental chamber to the X‑Y stage with the four thumb screws. Environmental chamber Onstage incubator master plate X‑Y stage Perfusion ports Thumb screw IMPORTANT! If you plan to use the perfusion ports on the environmental chamber, install the master plate such that the perfusion ports face towards the front of the system (as shown in the image above).
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Page 15: Set Up The Evos ™ Onstage Incubator (Osi-2) For Operation
Chapter 2 Methods Set up the EVOS Onstage Incubator (OSI-2) ™ Set up the EVOS Onstage Incubator (OSI-2) for operation ™ Follow the procedures below to set up the EVOS Onstage Incubator (OSI-2) for operation. For the ™ locations of various input jacks and gas ports, refer to “Control unit (back view)” page 8. - Page 16 Chapter 2 Methods Set up the EVOS Onstage Incubator (OSI-2) ™ Connect the power and system control lines 1. Plug the power cord into the power input jack on the control unit and the outlet. 2. Plug the USB cable into the USB control cable jack on the control unit and the USB port on the imaging system computer.
- Page 17 Chapter 2 Methods Set up the EVOS Onstage Incubator (OSI-2) ™ Set up the Environmental chamber for operation 1. Place the heated glass lid with the no-fog glass window on the incubator chamber. The heated glass lid is guided and attached in its place by the two magnets on its rim. Heated glass lid Incubator chamber Onstage incubator master...
- Page 18 Chapter 2 Methods Set up the EVOS Onstage Incubator (OSI-2) ™ 3. If desired, place the light shield cover on the light shield for fluorescence imaging applications. Note: The light shield cover completely blocks any ambient light from entering the environmental chamber and improves image quality in fluorescence imaging applications. 4.
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Page 19: Use The Onstage Incubator With The Evos M5000 Imaging System
Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ Use the Onstage Incubator with the EVOS M5000 Imaging ™ System Note: For EVOS M7000 Imaging System instructions, see “Use the Onstage Incubator with the ™ EVOS M7000 Imaging System” page 27. - Page 20 Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ Note: Alternatively, go to the Settings tab, click Incubator to expand the Incubator panel, then click Incubator Control to open the Incubator Control window. 5. Select Enable to initiate the control of the incubator. 6.
- Page 21 Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ 7. Select the desired Shutdown option: • Manual shutdown: The incubator remains on until you manually de-select the Enable option, then click Close. • Automatic shutdown: Select Shutdown after, then enter the time in Hours and Minutes that must elapse before the incubator is shut down automatically.
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Page 22: Optional: Set Ambient Temperature Offset
Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ Optional: Set Ambient Temperature Offset Ambient Offset enables you to adjust the ambient room temperature setting, which may be needed if condensation is observed on the glass lid. Review “Troubleshooting” on page 33 before you adjust the ambient temperature offset. -
Page 23: Configure Gas Inputs
Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ Configure gas inputs 1. Go to the Settings tab, then click Incubator to expand the Incubator panel. his SVG. uts or arrows Arrow_Libary. 2. Click Incubator Setup to open the Incubator Setup window. ... -
Page 24: Calibrate Incubator Oxygen Sensor
Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ 3. Select the appropriate options for the Gas Inputs that reflect the set-up for the EVOS Onstage ™ Incubator (OSI-2): • Port 1: Select Air or Premix, if not using the internal air compressor. If you select Premix, manually enter the percentage of the CO and O to reflect the specifics... - Page 25 Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ 2. Click Oxygen Sensor Calibration to open the Incubator Oxygen Sensor Calibration window. 3. Verify that the gas connections have been correctly configured. 4. Verify that the O % is set to a value similar to the types of conditions used, for example hypoxic or normoxic.
- Page 26 Chapter 2 Methods Use the Onstage Incubator with the EVOS M5000 Imaging System ™ 5. Select the purge gas source for the calibration: N or CO Note: A pure CO or N gas tank is required. 6. Click Begin Calibration. The EVOS M5000 Imaging System calibrates the oxygen sensor for the proper functioning of ™...
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Page 27: Use The Onstage Incubator With The Evos ™ M7000 Imaging System
Chapter 2 Methods Use the Onstage Incubator with the EVOS M7000 Imaging System ™ Use the Onstage Incubator with the EVOS M7000 Imaging ™ System Note: For EVOS M5000 Imaging System instructions, see “Use the Onstage Incubator with the ™ EVOS M5000 Imaging System” page 19. - Page 28 Chapter 2 Methods Use the Onstage Incubator with the EVOS M7000 Imaging System ™ 5. Select Use Incubator to initiate the incubator, then enter the target values for Temperature, CO and Oxygen. Select Use humidity to use a humidified atmosphere in the incubator chamber. ...
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Page 29: Configure Gas Inputs
Chapter 2 Methods Use the Onstage Incubator with the EVOS M7000 Imaging System ™ 7. Click Apply. Incubator status changes to Running and the control panel displays the Current, Target, and Actual values for Temperature, Humidity, CO , and Oxygen. 8. Click Done to return to the Capture tab. Note: Place an empty "dummy"... - Page 30 Chapter 2 Methods Use the Onstage Incubator with the EVOS M7000 Imaging System ™ SVG. or arrows ow_Libary. 3. Click Done after you have configured the gas connections for each port. 4. Open the gas cylinder valve, taking care to shut off or turn down the output pressure before opening.
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Page 31: Calibrate Oxygen Sensor
Chapter 2 Methods Use the Onstage Incubator with the EVOS M7000 Imaging System ™ Calibrate oxygen sensor Calibrating the oxygen sensor ensures that the atmosphere in the environmental chamber is replenished with the appropriate gasses in the correct proportion. Note: We recommend calibrating the oxygen sensor every 6 months or whenever precise control of oxygen is needed (e.g., for hypoxia experiments). - Page 32 Chapter 2 Methods Use the Onstage Incubator with the EVOS M7000 Imaging System ™ 4. Select the purge gas source for the calibtation: Nitrogen or CO Note: A pure CO or N gas tank is required. 5. Click Begin Calibration. The EVOS M7000 Imaging System automatically calibrates the oxygen sensor for the proper ™...
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Page 33: Chapter 3 Troubleshooting
Troubleshooting Troubleshooting Observation Resolution/Suggestion Cells appear unhealthy or are dying, or Check that the media and supplements are appropriate for the cell media turning the wrong color. type. Were any dyes, drugs, or other compounds added? If so, test a sample without them to see of they are the cause. - Page 34 Chapter 3 Troubleshooting Troubleshooting (continued) Observation Resolution/Suggestion Condensation under the stage, such Leakage of humid air from the environmental chamber due to a gap as on the objectives or on other metal near the sample. Check for gaps, such as from cut off corners of components.
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Page 35: Appendix A Specifications
Specifications Physical characteristics Note: Specifications of the EVOS Onstage Incubator (OSI-2) are subject to change without notice. ™ See the EVOS product page at www.thermofisher.com/evos for the latest product information. ™ Stagetop environmental chamber Control unit Length: 27 cm (11 in) 45 cm (18 in) 17 cm (6.8 in) 21 cm (8.3 in) -
Page 36: Appendix B Compatible Vessel Holders And Stage Plates
Compatible vessel holders and stage plates EVOS Onstage Incubator (OSI-2)-compatible vessel ™ holders and stage plates Capacity Catalog No. Adapter option Slides One Nunc Lab-Tek Chambered Coverglass AMEPVH055 ™ ™ One Nunc Lab-Tek II Chambered Coverglass AMEPVH039 ™... - Page 37 Appendix B Compatible vessel holders and stage plates EVOS Onstage Incubator (OSI-2)-compatible vessel holders and stage plates ™ (continued) Capacity Catalog No. Adapter option AMEPVH054 required for One Nunc multiwell dish AMEPVH040 EVOS M5000 Imaging ™ ™ System Dishes ...
- Page 38 Appendix B Compatible vessel holders and stage plates EVOS Onstage Incubator (OSI-2)-compatible vessel holders and stage plates ™ (continued) Capacity Catalog No. Adapter option Master/stage plates One BD Falcon T‑75 flask or one ™ 128.2 mm × 86.2 mm multiwell plate or AMEPVH054 AMEPVH001−AMEPVH020 ...
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Page 39: Appendix C Safety
Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document. -
Page 40: Location Of Safety Labels
Appendix C Safety Symbols on this instrument Location of safety labels Label and location s SVG. or arrows ow_Libary. Figure 6 Rear panel of EVOS Onstage Incubator (OSI-2) ™ ary. Figure 7 EVOS Onstage Incubator (OSI-2) stagetop environmental chamber ™ EVOS Onstage Incubator (OSI-2) User Guide ™... -
Page 41: Control And Connection Symbols
Appendix C Safety Symbols on this instrument Control and connection symbols Symbols and descriptions On (Power) Off (Power) Earth (ground) terminal Protective conductor terminal (main ground) Direct current Alternating current Both direct and alternating current Conformity symbols Conformity mark Description Indicates conformity with safety requirements for Canada and U.S.A. Indicates conformity with China RoHS requirements. -
Page 42: Safety Information For Instruments Not Manufactured By Thermo Fisher Scientific
Appendix C Safety Safety information for instruments not manufactured by Thermo Fisher Scientific Safety information for instruments not manufactured by Thermo Fisher Scientific Some of the accessories provided as part of the instrument system are not designed or built by Thermo Fisher Scientific. Consult the manufacturer's documentation for the information needed for the safe use of these products. -
Page 43: Safety Requirements For Evos ™ Onstage Incubator (Osi-2)
Appendix C Safety Instrument safety Safety requirements for EVOS Onstage Incubator (OSI-2) ™ WARNING! Thermo Fisher Scientific recommends the use of nitrogen, oxygen, and carbon dioxide gas with the Onstage Incubator. The use of alternative gasses is currently not supported and can adversely affect system performance. -
Page 44: Cleaning And Decontamination
Appendix C Safety Instrument safety Cleaning and decontamination CAUTION! Cleaning and Decontamination. Use only the cleaning and decontamination methods that are specified in the manufacturer user documentation. It is the responsibility of the operator (or other responsible person) to ensure that the following requirements are met: ·... -
Page 45: Safety And Electromagnetic Compatibility (Emc) Standards
Appendix C Safety Safety and electromagnetic compatibility (EMC) standards Safety and electromagnetic compatibility (EMC) standards The instrument design and manufacture complies with the following standards and requirements for safety and electromagnetic compatibility. Safety standards Reference Description EU Directive 2014/35/EU European Union “Low Voltage Directive” IEC 61010-1 Safety requirements for electrical equipment for measurement, control, and laboratory use –... -
Page 46: Environmental Design Standards
Appendix C Safety Chemical safety Environmental design standards Reference Description Directive 2012/19/EU European Union “WEEE Directive”—Waste electrical and electronic equipment Directive 2011/65/EU & European Union “RoHS Directive”—Restriction of hazardous substances in electrical Commission Delegated and electronic equipment Directive 2015/863 SJ/T 11364-2014 “China RoHS” Standard—Marking for the Restricted Use of Hazardous Substances in Electronic and Electrical Products For instrument specific certificates, visit our customer resource page at www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html. -
Page 47: Chemical Waste Safety
Appendix C Safety Chemical waste safety Chemical waste safety Chemical waste hazard CAUTION! HAZARDOUS WASTE! Refer to Safety Data Sheets (SDSs) and local regulations for handling and disposal. Chemical waste safety guidelines To minimize the hazards of chemical waste: • Read and understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste. -
Page 48: Biological Hazard Safety
Appendix C Safety Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. -
Page 49: Appendix D Documentation And Support
Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •... - Page 50 EVOS Onstage Incubator-2_UG_MAN0027987-v1-GUID- B55C8A2E-9840-4215-834E-022239185670-2022/05/18 21:23:26 en 20:50:01.849Z thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 6 March 2023...
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