Table of Contents
Advertisement
11
DETAILED OBSERVATION PROCEDURE
1
TURNING THE LAMP ON
-
0
Fig. 17
2
PLACING SPECIMEN ON THE STAGE
Fig. 18
3
ADJUSTING THE FOCUS
1
Fig. 19
7125000-795
1. Flip the main switch to "I" (ON) as shown in Figure 17.
2. Rotating the light intensity adjustment knob in the direction of
the arrow increases brightness and rotating knob in the oppo-
site direction decreases brightness. The intensity bar on the
knob indicates the direction of intensity level.
Place the specimen on the stage plate. In case the specimen is in
Petri dish, keep it on the petri dish holder.
In case you are using high Flask, remove the condenser front lens
by rotating it anti clock wise to fit in the flask. As shown fig. 18
In case your microscope has mechanical stage mounted and you
are using either glass slide or 90 well plate, use appropriate
holders.
Focusing Procedure (Figure 1 9)
1. Rotate the coarse adjustment knob (1) clockwise so that the
objective (3) is as close as possible to the specimen (We recom-
mend starting with10X).
2. While observing the specimen through the eyepieces, slowly
rotate the coarse adjustment knob (1) counterclockwise to
3
lower the objectives.
3. When coarse focusing of the specimen is obtained (an image is
2
observed), rotate the fine adjustment knob (2) for fine detail
focusing.
Working Distance (WD)
The WD refers to the distance between each objective and the
Specimen, when acute focus of the specimen is obtained.
Objective
Magnification
WD (mm)
TCM 400
4X
10X
20X
16
7.2
5.35
TCM 400
40X
2.0
18
Issue 1.3
Printed on April, 2022
Advertisement
Table of Contents
