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- Page 1 Instructions For Use TLA-110 Fixed-Angle Rotor For Use in the Beckman Coulter Optima MAX-XP, MAX-TL, MAX, MAX-E, TL, TLX, and TL-100 Tabletop Ultracentrifuges PN TL-TB-019AL April 2023 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 • In the UK, call us at +44 845 600 1345 • In Ireland, call us at +353 (01) 4073082 • In Italy, call us at +39 0295392 456 • In other locales, contact your local Beckman Coulter Representative. Find us on the World Wide Web at: www.beckman.com.
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Page 3: Revision History
Revision History This document applies to the latest and higher versions. When changes affect the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckman.com/techdocs and download the most recent manual or system help for your instrument. - Page 4 Revision History PN TL-TB-019AL...
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Page 5: Safety Notice
Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval. -
Page 6: Alerts For Warning, Caution, And Note
Although rotor components and accessories made by other manufacturers may fit in the TLA-110 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the TLA-110 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. -
Page 7: Safety During Installation And/Or Maintenance
Beckman Coulter Representative or returning parts to Beckman Coulter for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated. -
Page 8: Radiation Safety
Refer to applicable external device user’s manuals for operating procedures of that device. NOTE For Safety Data Sheets (SDS) information, go to the Beckman Coulter website at https://www.beckman.com/techdocs. WARNING Risk of chemical injury from bleach. To avoid contact with the bleach, use barrie protection, including protective eyewear, gloves, and suitable laboratory attire. -
Page 9: Mechanical Safety
Safety Notice Mechanical Safety WARNING Risk of personal injury or contamination. Before running the centrifuge with chemical or biological samples, test new labware types to determine if normal operation of the rotor may involve the use of materials that are toxic or otherwise biologically harmful. -
Page 10: Cleaning
Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your rotors for corrosion or damage. -
Page 11: Table Of Contents
Contents Revision History, iii Safety Notice, v Alerts for Warning, Caution, and Note, vi Safety Information for the TLA-110 Rotor, vi Safety During Installation and/or Maintenance, vii Radiation Safety, viii Chemical and Biological Safety, viii Mechanical Safety, ix Cleaning, x... - Page 12 Contents Storage, 20 Returning a Rotor, 20 Supply List, 21 Replacement Rotor Parts, 21 Other, 22...
- Page 13 Illustrations Illustrations Tube Cap Orientation for Microfuge Tubes, 4 Arranging Tubes in the Rotor., 4 Precipitation Curves for the TLA-110 Rotor, 14 CsCl Gradients at Equilibrium for the TLA-110 Rotor, 15 xiii...
- Page 14 Tables Tables Available Tubes for the TLA-110 Rotor, 7 Relative Centrifugal Fields for the TLA-110 Rotor when Using g- Max 3.5-mL Quick-Seal Tubes, 11 Relative Centrifugal Fields for the TLA-110 Rotor when Using 5.1-mL Quick-Seal Tubes, 12...
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Page 15: Tla-110 Fixed-Angle Rotor
TLA-110 Fixed-Angle Rotor Specifications Maximum speed 110,000 RPM 28° Density rating at maximum speed 1.7 g/mL Relative Centrifugal Field at maximum speed at r (48.5 mm) 657,000 g at r (37.2 mm) 504,000 g at r 353,000 g (26.0 mm) -
Page 16: Description
Beckman Coulter TLA-110 rotors are manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges. The TLA-110 fixed-angle rotor has a tube angle of 28 degrees from the axis of rotation. The rotor can centrifuge up to eight tubes. -
Page 17: Preparation And Use
Beckman Coulter. Use of other manufacturers’ components or accessories in the TLA-110 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor. - Page 18 TLA-110 Fixed-Angle Rotor Preparation and Use If using 1.5 mL Microfuge tubes, place the adapters into the tube cavities before inserting the tubes. a. Cut the hinges off the caps. b. Face the lift tab of all tubes toward the outside of the rotor (see...
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Page 19: Operation
TLA-110 Fixed-Angle Rotor Preparation and Use Use the required spacers, if necessary (see Table 1), to complete the loading operation. After the rotor is loaded, insert it into the portable polypropylene rotor vise (346133). Place the lid on the rotor and tighten it firmly to the right (clockwise) by hand. -
Page 20: Removal And Sample Recovery
Remove the lid by unscrewing it to the left (counterclockwise). Use a tube removal tool to remove the spacers and tubes. Tubes and Accessories The TLA-110 rotor uses tubes and accessories listed in Table 1. Be sure to use only those items listed, and to observe the maximum speed limits shown. -
Page 21: Temperature Limits
• If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior to centrifugation. Table 1 Available Tubes for the TLA-110 Rotor Tube Required Accessory Max. -
Page 22: Optiseal Tubes
Quick-Seal tubes and floating spacers (also called g-Max spacers). This means that you can run the shorter tubes listed in the table in the TLA-110 rotor without reduction in g force. Additional information about the g-Max system is available in publication DS-709B. -
Page 23: Microfuge Tubes
Table Run Times The k factor of the rotor is a measure of the rotor’s pelleting efficiency. Beckman Coulter has calculated the k factors for all of its preparative rotors at maximum rated speed and using full tubes. The k factor is calculated from the formula: EQ 1 ⁄... -
Page 24: Run Speeds
TLA-110 Fixed-Angle Rotor Preparation and Use Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows: EQ 4 110,000 --------------------------------------- - actual run speed Run times can also be estimated from data established in prior experiments if the k factor of the previous rotor is known. - Page 25 TLA-110 Fixed-Angle Rotor Preparation and Use Table 2 Relative Centrifugal Fields for the TLA-110 Rotor when Using g-Max 3.5-mL Quick-Seal Tubes Relative Centrifugal Field ( g) At r At r At r Rotor Speed (RPM) (48.5 mm) (37.2 mm) (26.0 mm)
- Page 26 TLA-110 Fixed-Angle Rotor Preparation and Use Table 3 Relative Centrifugal Fields for the TLA-110 Rotor when Using 5.1-mL Quick-Seal Tubes Relative Centrifugal Field ( g) At r At r At r Rotor Speed (RPM) (48.6 mm) (33.6 mm) (18.4 mm)
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Page 27: Selecting Cscl Gradients
Each curve in Figure 4 is within the density limits allowed for the TLA-110 rotor: each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities (one for each fill level) that avoid precipitation at that speed. - Page 28 TLA-110 Fixed-Angle Rotor Selecting CsCl Gradients Figure 3 Precipitation Curves for the TLA-110 Rotor 1.90 1.80 1.70 1.60 1.50 TLA-110 ROTOR 1.40 = 20°C = 4°C 1.30 1.20 Rotor Speed (K RPM) * Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation.
- Page 29 TLA-110 Fixed-Angle Rotor Selecting CsCl Gradients Figure 4 CsCl Gradients at Equilibrium for the TLA-110 Rotor TLA-110 ROTOR = 20°C = 4°C 18.0 21.7 25.5 29.2 32.9 36.7 40.4 44.2 47.9 Distance from Axis of Rotation (mm) * Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds (from...
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Page 30: Typical Examples For Determining Cscl Run Parameters
TLA-110 Fixed-Angle Rotor Typical Examples for Determining CsCl Run Parameters Typical Examples for Determining CsCl Run Parameters Example A: A separation that is done frequently is the banding of plasmic DNA in cesium chloride with ethidium bromide. The starting density of the CsCl solution is 1.55 g/mL. In this separation the covalently closed, circular plasmid bands at a density of 1.57 g/mL, while the nicked and linear species band at... - Page 31 TLA-110 Fixed-Angle Rotor Typical Examples for Determining CsCl Run Parameters centerband-to-centerband separation. This interband distance, d , can be calculated from the formula: EQ 7 θ ----------- - θ where d is the interband distance when the tube is held at an angle, , in the rotor.
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Page 32: Care And Maintenance
Replace O-rings about twice a year or whenever worn or damaged. Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. - Page 33 TLA-110 Fixed-Angle Rotor Care and Maintenance The Rotor Cleaning Kit contains two plastic-coated brushes and two quarts of Solution 555 for use with rotors and accessories. NOTE Do not wash rotor components in a dishwasher. Do not soak in detergent solution for long periods, such as overnight.
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Page 34: Decontamination
Ethanol is a flammability hazard. Do not use it in or near operating ultracentrifuges. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 35: Supply List
Customers located outside the United States should contact their local Beckman Coulter office. Supply List To order parts and supplies or obtain publications referenced in this manual, call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada) or contact your local Beckman Coulter Representative. -
Page 36: Other
TLA-110 Fixed-Angle Rotor Supply List Other NOTE For MSDS information, go to the Beckman Coulter website at www.beckman.com. Description Part Number Tubes and accessories Table 1 Tube rack 355872 Quick-Seal Cordless Tube Topper kit, 60 Hz 358312 Quick-Seal Cordless Tube Topper kit, 50 Hz (Europe) - Page 37 3. This warranty covers the Beckman Coulter Centrifuge Systems only (including but not limited to the centrifuge, rotor, and accessories) and Beckman Coulter shall not be liable for damage to or loss of the user’s sample, non-Beckman Coulter tubes, adapters, or other rotor contents.
- Page 38 Beckman Coulter will repair or replace the rotor free of charge. 4. If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube, bottle, tube cap, spacer, or adapter, covered under the Conditions of this Warranty, Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point (2) above, and the replacement policy.
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Page 41: Related Documents
Related Documents Rotors and Tubes for Beckman Coulter Tabletop Preparative Ultracentrifuges (TLR-IM-9) • Rotors Tubes and Accessories • • Using Tubes and Accessories • Using Rotors Care and Maintenance • • Chemical Resistances • The Use of Cesium Chloride Curves Gradient Materials •...
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