Knauer Azura P 2.1S User Manual
  1. Manuals
  2. Brands
  3. Knauer Manuals
  4. Water Pump
  5. AZURA P 2.1S
  6. User manual

Knauer Azura P 2.1S User Manual

Hide thumbs Also See for Azura P 2.1S:
Table of Contents

Advertisement

Quick Links

Pump P 2.1S/P 4.1S
User Manual
V6870
HPLC

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Azura P 2.1S and is the answer not in the manual?

Questions and answers

Related Manuals for Knauer Azura P 2.1S

Summary of Contents for Knauer Azura P 2.1S

  • Page 1 Pump P 2.1S/P 4.1S User Manual V6870 HPLC...
  • Page 2 Note: The contents of this user manual apply for the BlueShadow product as well. Please submit any request on article numbers for BlueShadow.

  • Page 3: Table Of Contents

    Table of Contents Table of Contents Note: For your own safety, read the manual and always observe the warnings and safety information on the device and in the manual! Intended Use..........5 Device Overview .
  • Page 4 Table of Contents Switching Off the Pump ........31 Functionality Tests .

  • Page 5: Intended Use

    Intended Use Intended Use Note: Only use the device for applications that fall within the range of the intended use. Otherwise, the protective and safety equipment of the device could fail. Device Overview The HPLC pumps P 4.1S/ P 2.1S with pump heads can be used as feed pumps or dosing pumps in analytical or preparative applications.

  • Page 6: Features

    Intended Use Legend LAN port Opening of the fan Pin header for remote control CE mark Power connection - bushing Serial number Hole for the ground connection RS-232 port External devices like computers, fraction collectors, etc. can be connected in 3 different ways: ...

  • Page 7: Pump Heads

    Intended Use  Piston backflushing  High physical and chemical stability  Flexible control with LAN connection, RS-232 interface, and analog control signals  Control with chromatography software Options A pump in combination with another pump can be used to set up a binary high pressure gradient system.

  • Page 8: Eluents

    Intended Use Eluents Even small quantities of other substances, such as additives, modifiers, or salts can influence the durability of the materials. Note: The list of selected solvents was compiled based on research in the perti- nent literature and is only a recommendation. If there is any doubt, contact the Technical Support of the manufacturer.

  • Page 9: Scope Of Delivery

    Dilute sodium hydroxide (1M)  Water 1. valid for the specified temperature range Scope of Delivery Note: Only use original parts and accessories made by KNAUER or a company authorized by KNAUER.  Pump P 2.1S/P 4.1S  User manual ...
  • Page 10 The environmental, installation, and connection specifications in the user manual  National and international regulations pertaining to laboratory work  Original spare parts, tools, and solvents made or recommended by KNAUER  Good Laboratory Practice (GLP)  Accident prevention regulations published by the accident insurance compa- nies for laboratory work ...

  • Page 11: Signal Words

    Decontaminatin Report Devices without a completed Decontamination Report will not be repaired. If you would like to return a device to KNAUER, make sure to enclose a completed Decontamination Report with the device: http://www.knauer.net/en/down- loads/service.html...

  • Page 12: Symbols And Signs

    Unpacking and Setup Contacting the Technical Support You have various options to contact the technical support: Phone:+49 30 809727-111 Fax:+49 30 8015010 E-mail:support@knauer.net Location Requirements Requirements The location for the device must meet the following requirements: Device defect The device overheats at exposure to sunlight and insufficient air circulation.

  • Page 13: Power Supply

    Unpacking and Setup  Weight 1.5 kg  Dimensions 105 × 100 × 185 mm (Width × Height × Depth)  Power supply 24 V DC  Air humidity < 90 %  Temperature 4 – 40 °C (39.2 – 104 °F) ...

  • Page 14: Connecting The Eluent Line To The Pump Head

    Unpacking and Setup 3. Open the accessories kit and take out all accessories. In case any parts are missing, contact the technical support. Grip the device at its side panels and lift it out of the packaging. 4. Remove the foam inserts from the device. Pull off the antistatic bag, if neces- sary.

  • Page 15: Control

    Unpacking and Setup Next steps: Integrate the pump into the HPLC flow system. Control Fig. 6 Pump, rear view Legend LAN port CE mark Pin header for remote control Opening of the fan Power connection - bushing Serial number Warning 1 RS-232 port Hole for the ground connection Pin Header for Remote Control...
  • Page 16 Unpacking and Setup Contact data Explanation ERROR IN/OUT TTL-compatible input  min. 10 mA  Low active After receiving a signal (e. g. short-circuit to ground) from an external device, an error message appears and the device stops. ANALOG IN Flow rate is controlled through external control voltage (0–10 V).

  • Page 17: Computer Control

    Unpacking and Setup Process Figure 1. Place the terminal strip on a suitable surface. 2. Push the depressor tool into the opening on the upper side. 3. Continue pushing the depressor tool down and lead the cable into the front end of the termi- nal strip.

  • Page 18: Connecting The Cables

    Unpacking and Setup  In case you use an USB-to-COM box, the option "Allow the computer to turn off ths device to save power" in the devicemanager must be deactivated for all USB hosts.  Only for Windows 7: For the network adapter, the option "Allow the com- puter to turn off this device to save power"...

  • Page 19: Configuring The Router

    Unpacking and Setup  There is a patch cable for each device and the computer. Process: 1. Use the patch cable to connect the router and the computer. Repeat this step to connect all devices. 2. Use the power supply to connect the router to the mains power system. Configuring the Router The router is preset at the factory.

  • Page 20: Ground

    If the supplied power adapter is used, then the ground connection remains unused.  Please contact the technical support of KNAUER, if the pump along with other devices should be connected to the power supply with a 6-prong power adapter; a pump needs to be grounded exclusively.

  • Page 21: Selecting The Pump Head

    Operation 3. Switch on the power adapter. 4. Wait until the pump has completed the self-test. Result: After the device is switched on, the display shows Pump and the Firmware version. The device performs a self-test. After all tests have been successfully completed, the status of the pump with its current flow rate is displayed.

  • Page 22: Control

    Operation Process Figure 1. Prime liquid with the syringe. 2. Hold down start/stop key until flushing starts. Control The pump can be operated in two ways:  Using the buttons at the device ® ®  Using the software ClarityChrom or ChromGate Note: Operator errors and clogged capillaries can cause high pressure spikes.
  • Page 23 Operation dent on the compressibility and the viscosity of the used solvent and on the pump. Therefore, it must be determined individually for each pump. The flow rate can be altered while the pump is in operation. Practical Tip: Hold down both arrow keys to expedite changing the values. Process Figure 1.
  • Page 24 (e. g. if leaking). The pump is preset to a standard value for the maximum power consumption. KNAUER recommends that with smaller flow rates the standard value for the maximum power consumption should be insignificantly decreased.

  • Page 25: Software

    Operation 3. When the cursor flashes, use the arrow keys again to set the value for the maximum power consump- Fig. 15 Minimum power consumption tion. 4. Hold down left arrow key. Press right arrow key once. 5. When the cursor flashes, use the arrow keys again to set the value for the minimum power consump- Fig.
  • Page 26 Operation Via Local Area Network (LAN) A device connected to a LAN is recognized by the software and automatically receives an IP address because it is set to Dynamic Host Configuration Protocol (DHCP) at the factory. Prerequisite:  Device has been connected to LAN. ...
  • Page 27 Operation Control com- Range and specifi- Description mand cation ADJ10(?) RD/WR 100 – 2000 Adjust parameter for 10 ml pump head ADJ50(?) RD/WR 100 – 2000 Adjust parameter for 50 ml pump head CORR10(?) RD/WR 0 – 300 Correction parameter for 10 ml pump head CORR50(?) RD/WR 0 –...
  • Page 28 Operation Control com- Range and specifi- Description mand cation EXTFLOW? IMOTOR? RD 0 – 100 Motor current in relative units LOCAL Put the instrument in local mode REMOTE Put the instrument in remote mode ERRORS? Returns 5 last saved errors Start flow Stop flow Changing to Analog Control (before firmware version 1.37)
  • Page 29 Operation In case you change back to RS-232 or LAN, you are re-setting the standard set- tings. The pump does not start with activated flow. The flow stops if the short- circuit connection GROUND and START IN is active. Prerequisite: ...
  • Page 30 Operation Starting with a Short-Circut Connection for the short circuit (or TTL-low) for interrupting and continuing the operation of the pump. The operation of the pump is dependent on the STARTLEVEL setting:  STARTLEVEL 1 (standard setting RS-232, LAN): The pump does not operate during the time of short-circuit.

  • Page 31: Switching Off The Pump

    Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a provider autho- rized by KNAUER performs this functionality test during the installation. The...

  • Page 32: Troubleshooting

    Troubleshooting Execution The test can be carried out either by the Technical Support of KNAUER or from a provider authorized by KNAUER. Troubleshooting First measures for troubleshooting:  Check all screw fittings  Check whether air has gotten into the supply lines ...

  • Page 33: Error List And Solutions

    • Clean the check valves • Exchange the check valves • If the pump head seals are defective, solvent enters the piston backflushing; inform the technical support of KNAUER. Pressure or flow rate Check the following options: fluctuations • Purge the pump head so as to remove the air bub- bles •...

  • Page 34: Maintenance And Care

    • Pump without pressure sensor: Pay attention to the influence of the pressure on the flow rate (will not be compensated). • Inform the Technical Support of KNAUER. Pump will not turn Power cable needs to be connected to power supply and power adapter has to be turned on.

  • Page 35: Contacting The Technical Support

    Maintenance Contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract: • Opening the device or removing housing parts. Pump Head Removing the Pump Head Prerequisite: The pump head has been flushed with suitable solvent.

  • Page 36: Installing The Pump Head

    Maintenance and Care Process Figure 1. Unscrew inlet fitting and outlet fitting 2. Unscrew the inlet fitting to the eluent. 3. Unscrew the outlet fitting to the pressure sensor. 4. Alternately unscrew the 4 fastening screws 5. Hold the pump head and consecu- tively pull out all fastening screws.

  • Page 37: Removing The Check Valves

    Maintenance and Care Fig. 24 Check valve Removing the Check Valves The pump head is equipped with two check valves. Prerequisite:  The pump head has been purged.  The capillary and tubing have been removed.  The pump head has been removed. Tools: Open-end wrench, size 13 Process Figure...

  • Page 38: Installing The Check Valves

    Maintenance and Care Installing the Check Valves Insert the check valves in the direction of the flow. The notch of the check valve points downward. Insert the NP check valves in the direction of the flow. The arrow on the NP check valve points upward. Prerequisite: The check valves have dried.

  • Page 39: Technical Data

    Technical Data Technical Data Solvent delivery Pump type Dual-piston pump with main and auxiliary piston  Flow rate range 10 ml pump head: 0.001 - 10 ml/min  50 ml pump head: 0.01 - 50 ml/min  Maximum pressure 10 ml pump head: 40 MPa to 10 ml/min Imax = 70 ...

  • Page 40: Accessories And Spare Parts

    Accessories and Spare Parts Technical parame- Temperature range 4 – 40 °C; 39.2 – 104 °F ters Humidity below 90 % non-condensing General Power supply 100 – 240 V; 50 – 60 Hz  Dimensions Pump without pressure sensor: 220 × 121 × 138.1 mm ...

  • Page 41: Available Pump Heads

    Legal Information Name Order number Pump P 2.1S with 10 ml pump head (stainless steel) APG90EA Pump P 2.1S with 10 ml pump head (ceramic) APG90EB Pump P 2.1S with 10 ml pump head (Hastelloy-C) APG90EC Pump P 2.1S with 50 ml pump head (stainless steel) APG90FA Pump P 2.1S with 50 ml pump head (ceramic) APG90FB...

  • Page 42: Declaration Of Conformity

    KNAUER are marked as waste electrical and elec- tronic equipment: 160214. WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to category 8 and 9, which, among others, comprise laboratory equipment.
  • Page 43 Abbreviations and Terminology Term Explanations Good Laboratory Practice - quality assurance for laborato- ries High Pressure Gradient. Operating mode of an HPLC sys- tem. The solvent is mixed on the high pressure side of the pump. HPLC High Performance Liquid Chromatography. Solvent Mobile phase (eluent) or carrier for liquid chromatography Remote...

  • Page 44: Index

    Index Index Location Accessories Additives Maintenance AVV marking maintenance contract Modifiers Cap fitting Care Operation Check valves Arrow keys Cleaning Exchanging Removing Cleaning port (LAN) Connectors on the Rear Side power cable Contact power strip contamination Power supply Controlling the pump power supply professional group Pump control...
  • Page 45 Index Steuerung der Pumpe Switch-on Technical Data Technical data Technical support test Installation Qualification Operation Qualification transport damage Warning instructions warranty P 2.1S/P 4.1S user manual V6870, version 3.1...
  • Page 46 Index P 2.1S/P 4.1S user manual V6870, version 3.1...
  • Page 47 © KNAUER Wissenschaftliche Geräte GmbH All rights reserved. The information in this document is subject to change without prior notice. Original edition/Translation of the original edition of this manual, version 1.0 Last update: 29.08.2016 Printed in Germany on environmentally friendly paper from sustainable forests.

This manual is also suitable for:

Azura p 4.1s

Table of Contents