Knauer BlueShadow Pump 80P Instructions Manual
  1. Manuals
  2. Brands
  3. Knauer Manuals
  4. Water Pump
  5. BlueShadow 80P
  6. Instructions manual

Knauer BlueShadow Pump 80P Instructions Manual

Hide thumbs Also See for BlueShadow Pump 80P:
Table of Contents

Advertisement

Quick Links

Pump 80P
Instructions
HPLC
HPLC
Document no. V7685

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the BlueShadow Pump 80P and is the answer not in the manual?

Questions and answers

Baba Odangla
June 7, 2025

How can you effectively prime the 80p pump to achieve consistent volume

0

Subscribe to Our Youtube Channel

Related Manuals for Knauer BlueShadow Pump 80P

Summary of Contents for Knauer BlueShadow Pump 80P

  • Page 1 Pump 80P Instructions HPLC HPLC Document no. V7685...
  • Page 2 Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone: +49 30 809727-111 (workdays 9-17h CET)

  • Page 3: Table Of Contents

    Scope of delivery ............. .11 BlueShadow Pump 80P Instructions V7685...
  • Page 4 Create program ............39 BlueShadow Pump 80P Instructions V7685...
  • Page 5 Storing the pump head ..........57 BlueShadow Pump 80P Instructions V7685...
  • Page 6 Spare parts ..............66 BlueShadow Pump 80P Instructions V7685...

  • Page 7: General

    These operating instructions are an integral part of the device. It must be kept in the immediate vicinity of the device and accessible to the user at all times. You can download these and other instructions from the KNAUER web- site: www.knauer.net/library...

  • Page 8: Legal Information

    1.4.4 Warranty seal A blue or orange warranty seal is affixed to some devices. A blue seal is used by KNAUER’s Manufacturing or Customer Support ƒ for devices to be sold. After repair, service technicians attach an orange seal onto the identi- ƒ...

  • Page 9: Basic Safety Instructions

    Operation in potentially explosive areas without special and additio- ƒ nal explosion protection. Contact the KNAUER Customer Support for more information. User qualification The user is qualified to handle the device if all of the following points apply: He has at least a basic knowledge of liquid chromatography.

  • Page 10: Operator Responsibility

    ƒ Protective gloves in accordance with the prevailing ambient conditions ƒ and used solvents (e.g. heat, cold, protection against chemicals). Lab coat ƒ Personalised protective safety equipment which is specified in the ƒ particular laboratory. BlueShadow Pump 80P Instructions V7685...

  • Page 11: Working With Solvents

    In case of leakage: Switch off the system. Only take the device into ƒ operation if the cause of the leak has been resolved (see chapter 8 on page 50). BlueShadow Pump 80P Instructions V7685...

  • Page 12: Specific Environments

    ƒ system in order to avoid dead volumes, not exactly fitting connections and spreading contamination. Safety features: The device may only be opened by the KNAUER Cus- ƒ tomer Support of KNAUER or any company authorized by KNAUER (see chapter 1.4.1 on page 2).

  • Page 13: Service Request Form And Decontamination Report

    Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired. If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www.knauer.net/servicerequest BlueShadow Pump 80P Instructions V7685...

  • Page 14: Product Information

    LPG system: Connection of a binary or ternary LPG valve block ƒ Ultra-precise gradient mixtures throughout entire flow range ƒ High physical and chemical stability ƒ Direct pump control via analog and digital signals ƒ Control with chromatography software ƒ BlueShadow Pump 80P Instructions V7685...

  • Page 15: Device Overview

    Fig. 1: Front view Legend Socket for the LPG valve block Events connector Opening of the fan Warranty seal Power switch Power supply CE-mark Remote connector Interface RS-232 LAN connection Serial number Fig. 2: Rear view BlueShadow Pump 80P Instructions V7685...

  • Page 16: Pump Heads

    Number of cycles and operating times — Limit values of the pump head parameters All measuring data archived in accordance with GLP (Good Laboratory ƒ Practice). Transfer of all data to the software and pump display. ƒ BlueShadow Pump 80P Instructions V7685...

  • Page 17: Symbols And Signs

    A warranty seal is affixed to some devices. For more information see chap. 1.4.4 on p. 2. Scope of delivery Note: Only use original parts and accessories made by KNAUER or a company authorized by KNAUER. Power cable ƒ BlueShadow Pump 80P with pump head ƒ...

  • Page 18: Installation

    As an alternative, allow the device to warm up in the laboratory for at ƒ least 3 hours and do not connect to the power supply during this dura- tion to avoid damages by condensed water. BlueShadow Pump 80P Instructions V7685...

  • Page 19: Power Supply

    Electronic defect Electronic hazard when using an identically constructed power adapter from another manufacturer. Only use spare parts and accessories from KNAUER or a company authorized by KNAUER. Conditions The electrical power supply at the installation site must be connected ƒ...

  • Page 20: Connecting The Piston Backflushing

    Upon switch-on: The rear piston area of the pump head is flushed ƒ automatically for 15 seconds. In continuous mode: The rear piston area of the pump head is flushed ƒ automatically every 30 minutes, for 15 seconds. BlueShadow Pump 80P Instructions V7685...

  • Page 21: Eluent Inlet

    PEEK or stainless steel parts are available. The eluent lines are connected by the eluent inlet to the pump head. Legend Knurled-head screw Eluent inlet Fig. 5: Connecting the eluent inlet to the pump head BlueShadow Pump 80P Instructions V7685...

  • Page 22: Connecting The Eluent Inlet To The Pump Head

    2. Turn the eluent inlet until the intake manifold input points forward. 3. Tighten the knurled-head screw. Fig. 6: Eluent inlet, variant 1 Fig. 7: Eluent inlet, variant 2 BlueShadow Pump 80P Instructions V7685...

  • Page 23: Connecting The Eluent Line

    Pump head Process Figure 1000 ml 1. Push the Teflon hose directly ƒ on the olive-type tube fitting 2. Fasten the Teflon hose with hose clamp. Fig. 9: Connecting the eluent line to the 1000 ml pump head BlueShadow Pump 80P Instructions V7685...

  • Page 24: Changing The Setup To Lpg

    5. Connect the eluent tubing to the inlets of the LPG Fig. 11: Valve block connection valve block. 6. Plug a cap fitting into the unused inlet. BlueShadow Pump 80P Instructions V7685...

  • Page 25: Controlling The Lpg Module

    Operation on display ƒ All connectors are located on the rear side of the detector. Legend Socket for the LPG valve block Events connector Remote connector Interface RS-232 LAN connection Fig. 13: Rear view with connectors BlueShadow Pump 80P Instructions V7685...

  • Page 26: Pin Header Connectors

    4TTL TTL-compatible output Levels: passive 0 V ƒ active 5 V ƒ Pulse: 5 V for at least 1000 ms 5TTL TTL-compatible output Levels: passive 0 V ƒ active 5 V ƒ Pulse: 5 V for at least 1000 ms ƒ BlueShadow Pump 80P Instructions V7685...
  • Page 27 10 V according to maximum flow rate Start GND Reference point of the voltage at the signal inputs. TTL Output Start OUT Levels: passive 5 V ƒ active 0 V ƒ Output is active for 500 ms when a program starts. BlueShadow Pump 80P Instructions V7685...

  • Page 28: Events Connector

    EVENT. Protection: 5 V–50 mA Reference point of the voltage at the signal inputs. 4.5.2 Events connector Sending control signals (Events) to external devices ƒ opening and closing contacts ƒ activating 500 ms pulses ƒ Symbol Explanation Pulse BlueShadow Pump 80P Instructions V7685...

  • Page 29: Connecting Cables To The Pin Header

    Connecting cables to the multi-pin connector of a switched on device cau ses a short circuit. Turn off the device before connecting cables. Pull the power plug. Electronic defect Electrostatic discharge can destroy the electronics. Wear a protective bracelet against electrostatic discharge and ground. BlueShadow Pump 80P Instructions V7685...

  • Page 30: Connecting The Device To A Computer

    4.5.4 Connecting the device to a computer Note: HPLC devices from KNAUER only work with IP addresses, which have been assigned by IPv4. IPv6 is not supported. This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network.

  • Page 31: Configuring The Lan Settings

    To connect the router to a LAN, an additional patch cable is required, which is not supplied within the scope of delivery. Prerequisites The computer has been switched off. ƒ There is a patch cable for each device and the computer. ƒ BlueShadow Pump 80P Instructions V7685...

  • Page 32: Configuring The Router

    Procedure 1. Find out port number and change it on the device. 2. Enter the port number in the chromatography software. Result The connection is established. BlueShadow Pump 80P Instructions V7685...

  • Page 33: Analog Control

    Installation 4.5.10 Analog control Analog ports serve for exchanging analog control signals. Reference point for the signals is the connector GND. OUT: Device sends signal. IN: Device receives signal. BlueShadow Pump 80P Instructions V7685...

  • Page 34: Operation

    Purging the pump Column defect Damage to the column due to purging. Open the venting screw. Remove the column. Before the pump can be used, it must be purged to remove excess air in the capillaries. BlueShadow Pump 80P Instructions V7685...

  • Page 35: Piston Backflushing

    Functional principle The piston backflushing function automatically flushes the rear piston area of the pump head upon switch-on and in continuous mode. BlueShadow Pump 80P Instructions V7685...

  • Page 36: Switch On And Self Test

    Flushing program — Standby and Wake up (WU) program — Program sequences ( Link menu) — Checking the system status as part of quality assurance measures in accordance with good laboratory practices ( GLP menu). BlueShadow Pump 80P Instructions V7685...

  • Page 37: Led Status

    Blue Device in standby. 5.5.2 RFID icon The status of a RFID valve is shown on the display in the main display. RFID Status Icon RFID tag found. Icon flashing RFID tag not found BlueShadow Pump 80P Instructions V7685...

  • Page 38: Operating With The Keypad

    Start and stop Starts or stops the pump button with set values. Purge button Opens purge option. Standby button Starts standby mode. ƒ Inserts a decimal ƒ point if numeric pad is activated. BlueShadow Pump 80P Instructions V7685...

  • Page 39: Menu Structure

    If you want to use the numeric pad, simply press the confirm button to activate numeric pad. A decimal point is located on the standby key in case the numeric pad is activated. BlueShadow Pump 80P Instructions V7685...

  • Page 40: Menu Structure: Overview

    Operation 5.6.2 Menu structure: overview Fig. 19: Menu structure overview BlueShadow Pump 80P Instructions V7685...

  • Page 41: Device Setting Parameters

    The selection is used as a basis for calculating the flow rate and determines the maximum system pressure. This value applies for all ope- rating modes and cannot be exceeded. BlueShadow Pump 80P Instructions V7685...
  • Page 42 None (isocratic) Channel A-D: ON/OFF Acti- ƒ vate up to four channels Note: The gradient modus is displayed as A, B, C or D with filled background in the lower left area of the main window. BlueShadow Pump 80P Instructions V7685...
  • Page 43 — Disabled Time constant OUT: Set the ƒ time constant for analog output to be used for signal smoothing. The larger the value of the selected time constant, the stronger the applied signal smoothing. BlueShadow Pump 80P Instructions V7685...
  • Page 44 Event check Testing the events of the pin header connectors. Start-IN and Error-IN: Status ƒ at the digital inputs. Active only: Connects Event 1 ƒ to Event 8 and 24 V. BlueShadow Pump 80P Instructions V7685...

  • Page 45: Glp Menu

    5. Enter the time value of the new line and adjust the respective parameters. 6. Press for two seconds to exit the submenu. The program is automatically saved. BlueShadow Pump 80P Instructions V7685...

  • Page 46: Run Program

    2. Select the program 00. 3. Activate the numeric pad by pressing enter the program number you wish to delete. 4. You will be asked whether you really want to delete the program. Confirm with or deny with BlueShadow Pump 80P Instructions V7685...

  • Page 47: Create A Program With A Starting Time

    If W1 (W = Wait) is used, the pump waits for an external start signal or for the user to press the upward key to set the status to run before exe- cuting this line. If the W0 setting is used, the link sequence runs without interruption. BlueShadow Pump 80P Instructions V7685...

  • Page 48: Run Link

    5.9.3 Delete link Process Figure 1. Select the menu Link and choose the link 00. 2. Activate the numeric pad by pressing enter the desired program number you wish to delete. 3. Confirm with or deny with BlueShadow Pump 80P Instructions V7685...

  • Page 49: Functionality Tests

    Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the technical support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation.

  • Page 50: Troubleshooting

    6. Replace the patch cable to the device with that no connection could be established. Has this been successful? ƒ 7. Make sure that the IP port of the device matches the port in the chro- matography software. BlueShadow Pump 80P Instructions V7685...

  • Page 51: Possible Problems And Solutions

    Technical Support. Flow rate is not Check the following options: correct Check the data for the solvent ƒ compressibility. Cleaning the check valves. ƒ Changing the check valves. ƒ BlueShadow Pump 80P Instructions V7685...

  • Page 52: System Messages

    First stop the link and then edit the data on gram from the the device display or with the chromatography running link software. Cannot initialize Check the cables and connections in the local area network. BlueShadow Pump 80P Instructions V7685...
  • Page 53 ƒ software. Insufficient access Change the entry. Invalid command Change the entry. ƒ Check the cable connections. ƒ Invalid parameter(s) Check the validity of the parameter(s). Invalid time in time Correct the time entry. table BlueShadow Pump 80P Instructions V7685...
  • Page 54 100 program lines are possible. Not supported Change the entry. Prg not compatible Modify the program or replace the pump head. with pump head! Program does not Create and edit a program. exist BlueShadow Pump 80P Instructions V7685...
  • Page 55 Wake up time Correct the entry for date or otherwise time. already passed! Change the line index in the link. Wrong Line number Wrong link Check Link-Nr. Values from 1-10 are possible ƒ Correct the entry. ƒ BlueShadow Pump 80P Instructions V7685...

  • Page 56: Maintenance And Care

    All smooth surfaces of the device can be cleaned with a mild, commer- cially available cleaning solution, or with isopropanol. Keypad The keypad of the modules can be cleaned with isopropanol and wiped dry with a soft, lint-free cloth. BlueShadow Pump 80P Instructions V7685...

  • Page 57: Checking The Fittings

    When buffers have been used, flush with water. ƒ When aggressive solvents have been used, flush with isopropanol. ƒ Note: For applications with normal phase, only use isopropanol as flush ing solution. BlueShadow Pump 80P Instructions V7685...

  • Page 58: Pump Head

    Note: Loosen the opposite pairs of fastening screws of the pump head evenly and alternately to prevent the pump pistons on the inside from jamming. BlueShadow Pump 80P Instructions V7685...

  • Page 59: Installing The Pump Head

    3. Screw tight the capillary screw fittings on the pump head and pressure sensor. 4. Screw the eluent lines into the eluent inlets. 5. Fasten the tubing of the piston backflushing at the inlet and out- let of the flush pump and the pump head. BlueShadow Pump 80P Instructions V7685...

  • Page 60: Check Valves

    1. Unscrew the outlet fittings 2. Remove the check valve 3. Unscrew the inlet fittings 4. Remove the check valve. Fig. 23: Removing the check valve Next Steps You can either replace or clean the check valves. BlueShadow Pump 80P Instructions V7685...

  • Page 61: Cleaning The Check Valve

    2. Manually screw in inlet and out let fittings and tighten them with a torque wrench and the respective torque. Fig. 24: Check valve Next step Re-install the pump head. BlueShadow Pump 80P Instructions V7685...

  • Page 62: Transport And Storage

    Transporting the device Carefully prepare the device for transport or storage. If you want to return your device to KNAUER for repairs, enclose the Service Request Form which can be downloaded from our website.

  • Page 63: Storing The Pump Head

    3. Fill the pump head with isopropanol. 4. Seal the inlets and outles with cap fittings. The pump head can be stored under the ambient conditions described in Storage conditions chapter „Technical data“ on page 59. BlueShadow Pump 80P Instructions V7685...

  • Page 64: Disposal

    160214. 10.2 WEEE registration number KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number classifies to category 8 and 9, which, among others, comprises laboratory equipment.

  • Page 65: Technical Data

    +/- 2 % at 5 – 50 % of the flow range with ethanol/water 10/90 Flow rate precision < 0,1 % RSD, measured at 5 – 50 % of the flow range with ethanol/water 10/90 BlueShadow Pump 80P Instructions V7685...

  • Page 66: Communication

    For indoor use only Line voltage fluctuations do not exceed ± 10 % of normal voltage. Overvoltage category II: Energy consumers are supplied by a fixed device. Permitted pollution degree Temperature range 4 – 40 ˚C; 39.2 – 104 ˚F BlueShadow Pump 80P Instructions V7685...
  • Page 67 Technical data Air humidity below 90 %, non-condensing Operating height max. 2000 meters above sea level BlueShadow Pump 80P Instructions V7685...

  • Page 68: Chemical Compatibility Of Wetted Materials

    The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter “Technical data”.
  • Page 69 This amorphous perfluorinated copolymer is inert against all commonly used solvents. However, it is soluble in perfluorinated solvents like Fluo- rinert® FC-75 and FC-40, and Fomblin perfluor-polyether solvents from Ausimont. In addition, it is affected by Freon® solvents. BlueShadow Pump 80P Instructions V7685...

  • Page 70: Non-Metals

    Synthetic ruby is monocrystalline alumina and gets its red color by the addition of some chromium oxide. It is biocompatible and very resistant to corrosion and wear. The material is characterized by a high hardness and a high thermal conductivity. BlueShadow Pump 80P Instructions V7685...

  • Page 71: Metals

    Titanium, titanium alloy (TiA16V4) Titanium has a low weight and a high hardness and stability. It stands out due to its very high chemical compatibility and biocompatibility. Titan is applied when neither stainless steel nor PEEK are usable. BlueShadow Pump 80P Instructions V7685...

  • Page 72: Repeat Orders

    500 ml pump head, titanium, accessories A4038V2 1000 ml pump head, stainless steel, accessories A4022-1 1000 ml pump head, titanium, accessories A4022V2 Check valve for 100 ml and 250 ml pump head A1122 Check valve for 500 ml and 1000 ml pump head A1080 BlueShadow Pump 80P Instructions V7685...
  • Page 73 2 x flat ribbon cable (1.5 m, 10-pos.) A1467 RS-232 connection cable A0895 Instruction kit 80P F4020V1.0 Accessory kit for 100 ml, 250 ml and 500 ml F4021 pump head Accessory kit for 1000 ml pump head F4022 BlueShadow Pump 80P Instructions V7685...
  • Page 74 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2020...

Table of Contents

Save PDF