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User Guide
Amicon
®
Stirred Cell Reservoir
Cat. No. 6028
Introduction
The 800 mL Amicon® Stirred Cell Reservoir increases the volume capacity of Amicon® Stirred
Cells. When pressurized by an external gas source, the reservoir automatically replenishes
liquid in the stirred cell due to the pressure differential between the two vessels. The
reservoir can also be used for continuous diafiltration to exchange sample buffer. In order
to maintain an appropriate volume of fluid in the Amicon® Stirred Cell, the Amicon® Stirred
Cell Selector Valve, cat. no. 6003, (or similar user-supplied valve(s) to control gas and liquid
output independently) should be installed between the reservoir and stirred cell.
Components Supplied
Spare inlet/
outlet fitting
O-rings
Pressure
relief valve
Amicon® Stirred
Cell Reservoir with
retaining stand.
Cap removal
tool
Tubing
(¼ in. outer diameter)
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Summary of Contents for Millipore Amicon 6028
- Page 1 User Guide Amicon ® Stirred Cell Reservoir Cat. No. 6028 Introduction The 800 mL Amicon® Stirred Cell Reservoir increases the volume capacity of Amicon® Stirred Cells. When pressurized by an external gas source, the reservoir automatically replenishes liquid in the stirred cell due to the pressure differential between the two vessels. The reservoir can also be used for continuous diafiltration to exchange sample buffer.
- Page 2 Setup Reservoir Assembly/Disassembly 1. Remove pressure relief valve (if installed) and slide reservoir out of retaining stand. Disassemble and clean all components prior to first time use. 2. Attach a 5 in. (12.7 cm) piece of ¼ in. (6.4 mm) outer diameter (OD) tubing to the liquid outlet port (labeled "L") on the bottom of the reservoir cap.
- Page 3 Setup, continued Additional tubing may be needed if using the reservoir with other devices. 1. Lay out the stirred cell, selector valve, reservoir, and four pieces of tubing in positions appropriate for their use together. Estimate length of tubing required for each connection and cut to length if necessary.
- Page 4 Operation WARNING: Do not exceed pressure limit of 5.2 bar (75 psi). During diafiltration, the pressure should not exceed 3.8 bar (55 psi), in order to maintain equilibrium between the stirred cell and the reservoir. If the liquid level increases slightly, concentrate briefly.
- Page 5 Pressure Equipment Directive. It has been designed and manufactured in accordance with sound engineering practices to ensure safe use. The product is accompanied by user instructions and bears markings to permit identification of EMD Millipore Corporation as the manufacturer or authorized representative of this product within the European Community. In compliance with Article 3 §...
- Page 6 The information in this document is subject to change without notice and should not be construed as a commitment by EMD Millipore Corporation (“Millipore”) or an affiliate. Neither EMD Millipore Corporation nor any of its affiliates assumes responsibility for any errors that may appear in this document.
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