Millipore Amicon Ultra-4 Series User Manual

User Guide
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Amicon
for volumes up to 4 mL
Amicon Ultra-4 3K, 10K, 30K, 50K, and 100K devices for research use only; not for use in diagnostic procedures
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Introduction
Amicon Ultra-4 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate
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recovery from dilute and complex sample matrices. The vertical design and available membrane surface area provide fast sample
processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration.
Typical processing time is 10 to 40 minutes depending on Molecular Weight Cutoff (MWCO). Solute polarization and subsequent fouling
of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and
potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipettor, while the ultrafiltrate is
collected in the provided centrifuge tube. The device can be spun in a swinging-bucket (for optimal performance) or fixed-angle rotor.
Amicon Ultra-4 devices are supplied nonsterile and are for single use only.
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The Amicon Ultra-4 product line includes 5 different cutoffs (MWCO):
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• Amicon Ultra 3K device — 3,000 MWCO
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• Amicon Ultra 10K device — 10,000 MWCO
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• Amicon Ultra 30K device — 30,000 MWCO
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• Amicon Ultra 50K device — 50,000 MWCO
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• Amicon Ultra 100K device — 100,000 MWCO
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Applications
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or
double-stranded), microorganisms, column eluates, and purified samples
• Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular
labels from a reaction mix, and protein removal prior to HPLC
• Desalting, buffer exchange, or diafiltration
Materials Supplied
The Amicon Ultra-4 device is supplied with a cap, a filter device, and a centrifuge tube.
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Required Equipment
• Centrifuge with swinging-bucket (preferred) or fixed-angle rotor with wells/carriers that can accommodate 17 mm × 124 mm 15 mL
conical-bottomed tubes
CAUTION: To avoid damage to the device during centrifugation, check clearance before spinning.
• Pipettor with 200 microliter (μL) tip for concentrate recovery
Amicon Ultra-4 Centrifugal Filter Devices
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Ultra-4 Centrifugal Filter Devices
Cap
Filter device
Centrifuge tube