Biotek 405 LS Operator's Manual page 91

create cleaning turbulence (at the specified height). The manifold descends to
aspirate again and ends with a final dispense to fill the wells.
Select the buffer to use, if applicable.
Buffer:
Bottom Wash Volume:
during the bottom wash.
Set the rate. Valid range is 3-11. The cell wash rates, 1 CW and 2 CW,
Flow Rate:
which use low-flow tubing, when available, are not recommended. Cell wash
options are designed for gentle washing, while bottom wash is designed for
vigorous washing.
Advanced Options
Parameters
Bottom Wash
Height:
Z-axis Position
Horizontal X-axis
Position
Horizontal Y-axis
Position
Delay start of
Vacuum
Enter the volume of wash solution to dispense per well
Description
The distance between the bottom of the aspirate
tubes and the carrier surface. The valid range is 12
to 175. The value in mm is displayed on screen.
The left and right position of the dispense tubes
when the carrier is beneath the manifold.
The front-to-back carrier position (or Y axis) to align
the microplate with the manifold tubes during a
dispense. The range depends on the model.
until dispensing this volume. 3000 µL max
Target Instrument Settings | 71
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