Biotek 405 LS Operator's Manual page 155

Microplate washer
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Required Materials:
Precision digital balance with a capacity of 400 g and readability of 0.01 g resolution
l
200 mL
Solution
l
runs.
See Washer Qualification Test Materials on page
The Excel spreadsheet provided on the operator's manual CD to perform the required
l
calculations: in the Verify Technology Spreadsheets folder, open
VerifyPlateReplacement.xls.
Prerequisites:
Make sure the replacement Verify Test Plate is clean and dry to begin this procedure.
l
Mix the dye solution, fill a buffer bottle attached to the washer, and fully prime the
l
system.
1. Perform Test Plate Alignment
LHC
1. Select
Tools>Instrument Utilities>Verify Manifold>Configure Utilities
2. Put the test plate on the carrier and click
This process updates configuration data needed to successfully run the Verify
routine.
2. Determine the Slope and Offset Volume Coefficients
This step is not mandatory but strongly recommended to ensure the most
n
reliable performance.
1. Put the Verify Test Plate on the balance and tare the balance.
2. Run the special procedure:
Touch screen
1. Select
Instrument>Other>Verify
.
Technology
2. Select
Plate Replacement
button in the Sensor Calibration box)
and press
Start
The 405 LS will perform the first part of the Verify process.
3. At the first pausing message, weigh the plate and record the
(W
) value.
A
#3: Dispense Precision solution. This volume is sufficient for two test
(select this
.
Replacement Procedure for Verify Test Plate | 135
155.
Test Plate Alignment
LHC
1. Select
Tools>Instrument
Utilities>Verify
Manifold>Configure Utilities
2. Click the
Sensor Calibration
link.
.
.
.
Aspirate Weight
BioTek Instruments, Inc.

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