Biotek 405 LS Operator's Manual page 204

184 | Chapter 7: Troubleshooting
Problem Possible Cause
Clogged aspiration
tubes on the
washer manifold.
Aspirate height
adjustment too
high or too low.
Vacuum pump
failure.
Uneven No surfactant in
aspiration of the buffer, such as
water buffer. Tween
Some wells
Insufficient
left full.
vacuum.
Protocol settings
not optimized.
Aspiration tubes
not properly
positioned
horizontally in
wells.
Microplate not
level in carrier, or
strips not level in
holder.
Too much Clogged vacuum
residual left filter.
in wells after
Waste bottle cover
aspiration.
not properly
sealed or fittings
not properly
connected.
Manifold out of
alignment or not
moving freely.
405™ Microplate Washer LS
What To Do
Remove and clean the washer manifold on page 131
Make sure the microplate carrier is level and the waste
valve is not touching the bench.
Change the aspiration height (Z-axis position) in the
protocol.
Contact BioTek TAC.
Add surfactant to the buffer. If this is not possible,
continue below.
®
20.
BioTek offers a high-flow pump for assays using only
water for the wash fluid. Contact BioTek for more
information.
Optimize protocols to improve evacuation on page 40
If none of the tubes are bent, try adjusting the
horizontal aspirate position (X-/Y-axis) in the protocol.
Reseat microplate in carrier or strips in holder.
Make sure the carrier is clean.
Try a different microplate or strip holder.
If the problem is unresolved, the carrier may have to
be realigned. Contact BioTek TAC.
If you are using an in-line vacuum filter (PN 49943),
the filter may need to be cleaned or replaced.
Firmly seat the waste bottle stopper. Make sure tubing
is connected properly.
Check for obstructions. If none are found, contact
BioTek TAC.