How To Shake The Plate - Biotek 405 LS Operator's Manual

3. Press the
Options
image of the column is filled it will be dispensed to. Conversely, when the
column image is blank or unfilled, the column will not be dispensed to.
4. Press to save the settings and continue.
Enter
Wash Step Format: Plate or Sector
When washing a high-density plate you can choose between two processing
methods, Plate or Sector:
Plate format performs each wash cycle to the entire plate before it starts the next
l
cycle.
Sector format performs the entire wash step to each sector of the plate before it
l
moves to the next sector. A sector is defined by the manifold's footprint:
96-tube manifolds process 384-well plates in 4 evenly spaced sectors (quadrants);
o
192-tube manifolds process 384-well plates in two sectors: even and odd
o
numbered columns.
Use sector mode when you are concerned about the fluid drying out before the
procedure is completed. To learn more
111

How to shake the plate

These instructions are for keypad control.
The shake command is tied to the
or incubating the plate at room temperature, as well as shaking.
There are two ways to specify a shake period:
During a wash cycle: To shake (and/or soak) in between every wash cycle,
l
select
OPTS
and follow the prompts to specify parameters.
Shake step: create a protocol to shake the plate, it can be a one-step, shake-
l
only protocol, or you can
See Create or Edit a Protocol (Keypad Only) on page 48
n
Soak is not the same as "submerge" the tips. You must define a prime step or
n
use the AutoPrime feature to soak the tips in the priming trough.
Soak is equivalent to incubating the plate at room temperature or delaying the
n
protocol.
key to toggle between filling the column or not. When the
See About Wash Processing Patterns on page
Soak
when defining
Wash Step Parameters
a Shake step before or after another step.
ADD
Introducing the 405 LS Keypad | 51
option. These instructions apply to soaking
and then select ,
MIDCYC
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