Biotek 405 LS Operator's Manual page 83

Keypad
Option
name
Filtration
Travel Rate: The rate at which the washer manifold
Positioning: X- and Y- horizontal axes, Z- height
Secondary
aspirate:
DISP Dispense
Flow Rate:
96-tube &
192-tube
manifolds
Description/Values range
When applicable, specify duration of
Time: vacuum filtration in seconds. 5-999
travels down into the wells. The selection
range is 1 to 5 for non-cell-based assays,
from slowest to fastest. With these rates,
the tubes slow their descent as they
approach the defined aspirate height (Z
Position) to aid complete evacuation of
the well.
For delicate, cell-based assays, the range
is 1CW (cell wash) to 4CW and 6CW.
These rates minimize turbulence in the
wells. The tubes descend at a constant
rate to the specified height. Rate 6CW
creates the least disturbance and
performs fastest.
Delay: Amount of time the tubes stay at the
aspirate height before lifting out of the
wells. Define a delay between 0 - 5000
ms. Increasing the delay may improve
evacuation of the wells.
(vertical) axis can be adjusted to improve
performance. Default Z-axis for 384-well
plates is 22 steps, 2 for 384-well PCR
plates.
Also called Crosswise Aspiration. First the
wells are aspirated using the position
defined above. The aspirate tubes rise and
then descend to the secondary position to
aspirate again.
The rate at which the fluid is dispensed
from the tubes. For cell-based assays, use
rate 1 or 2 for gentle washing with the 96-
tube manifold only. For normal
dispensing, the range is 3-11, 3 is slowest
and 11 is fastest.
Target Instrument Settings | 63
Default
values
30
3
0
Z = 29 for
96-well
plates;
X & Y = 0
No
7
BioTek Instruments, Inc.