Removing Protein Residuals And Fungi Growth - Biotek 405 LS Operator's Manual

124 | Chapter 4: Maintenance
6. You can
Submerge
Set in
Duration
After the dispense tubes have been primed, the manifold moves down into the
priming trough that is filled with the dispensed solution. The vacuum pump is
turned off and the tubes are allowed to soak. After the specified duration, the
vacuum pump is turned on and the trough is aspirated.
It takes approximately 93 mL of fluid to fill the priming trough. Be sure to
specify a volume of fluid that will cover the tubes.
7. Click to transfer the settings to the instrument.
Send
Keep in mind:
AutoPrime can be stopped when it is underway: press STOP on the keypad.
l
Any interaction with the instrument via the keypad or the LHC resets the
l
interval clock.
AutoPrime only runs when the main menu, quick menu, or run completion
l
message is displayed.

Removing Protein Residuals and Fungi Growth

Important! Solutions containing proteins, such as bovine serum albumin (BSA), will
compromise the 405 LS's performance over time unless a strict maintenance regime is
adhered to. Do not use isopropyl alcohol to flush out BSA.
When using protein solutions or similar fluids, BioTek recommends performing the
following additional Maintenance procedures to thoroughly flush out protein
particles and other contaminants from the fluid path.
Daily Practice with buffer or deionized water:
If the 405 LS will be idle between plates for longer than 45 minutes, flush the
proteins:
1. Fill a supply bottle with deionized water. Connect the bottle to the washer.
(Buffer valve "A" for Buffer Switching models)
2. Run the DAY_RINSE protocol.
3. Enable AutoPrime for 60-minute intervals.
At the end of the day:
1. Fill a supply bottle with deionized water. Connect the bottle to the washer
(Buffer valve "A" for Buffer Switching models).
405™ Microplate Washer LS
the manifold tubes in the fluid
and .
Hours Minutes
the , if desired.
after prime