Create An Autoclean Protocol - Biotek 405 LS Operator's Manual

2. Fill the "
Rinse Buffer
or with a wash buffer to leave the instrument primed and ready for use. Buffer
Switching models: specify the
3. Set the desired duration in minutes, up to 4 hours, to soak the tubes and run the
sonicator: use the arrow or
4. When you're ready, press
Warning! Ultrasonic energy is present in the cleaning reservoir when the AutoClean
program is running. Do not put your fingers in the bath! Ultrasonic energy can harm
human tissue.
Important! Ensure there is adequate room in the waste bottle and sufficient
n
volume in the supply bottle

Create an AutoClean protocol

Create an
AutoClean
tubing with the cleaning fluid. Approximately 93 mL of fluid fills the reservoir
during each run.
General Recommendation
deionized water to remove the detergent from the system and/or with a wash
buffer to leave the instrument primed and ready for use.
LHC Keypad
DEFINE>CREATE>NAME>PLATE>ADD>
Define the cleaning protocol:
1. First, add a
Prime
tubing with the cleaning fluid (or water).
2. Add an
AutoClean
3. : select the supply bottle containing the desired cleaning
Buffer Valve
fluid if Buffer Switching is installed.
4. : enter the duration, up to 4 hours. Input the time in minutes
Duration
when using the keypad; hours and minutes when using LHC.
5. Click to add a cleaning step to the protocol.
OK
" bottle with at least 305 mL of deionized or distilled water
RINBUF
key.
Options
.
Start
before running AutoClean!
protocol for regular use. Make sure it begins by priming the
: Run
AutoClean
step to fully prime the
step to the protocol.
( ) valve.
Rinse Buffer
for 1 hour. Follow with a full prime using
g
>ACLEAN
AutoClean the Washer | 127
BioTek Instruments, Inc.