Summary of Contents for Major Science SmartView Pro 1100
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SmartView Pro 1100 Imager System Instruction Manual Catalog Nos. UVCI-1100 www.majorsci.com service@majorsci.com Version 09H Revised on : 2019/07/22...
-2 × M7 nuts Signed by: Date: Major Science is liable for all missing or damaged parts / accessories within 7 days after customer received this instrument package. Please contact Major Science immediately regarding this issue. If no response within such time period from consignee party, that will be consignee...
Warning This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. NOTE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules.
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Major Science SmartView Pro Imager System series has been tested and found to comply with the limits for the CE regulation. Also, SmartView Pro Imager System series is RoHS compliant to deliver confident product which meets the environmental directive. These limits are designed to provide reasonable protection against harmful interference when the instrument series is operated in a commercial environment.
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Major Science makes no claim that its instruments are designed or certified as medical device; no presentation, promises, express warranty or implied warranty will be made concerning the suitability of these instruments for any medical use.
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or any connected apparatus. 4. ALWAYS make sure that your hands, work area, and instruments are clean and dry before making any connections or operating the power supply. 5. ONLY connect the power cord to a properly grounded AC outlet. Avoiding Damage to the Instrument 1.
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Symbols The symbols used on SmartView Pro 1100 Imager System are explained below. Indicates an area where a potential shock hazard may exist. Consult the manual to avoid possible personal injury or instrument damage. Indicate a warning of UV radiation.
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2. Preventative measures of risk Potential Risk Preventive measures Do not put the machine near the table edge. Bruise Move the machine by cart. Pinch Do not put your hands on the open door. Slash Prevent hard impact on the acrylic panel. UV radiation Do not open the darkroom door while you turn on the dangerous...
Section 1 Introduction 1.1 Overview SmartView Pro Imager System is the next generation of gel documentation instrument. It is specifically designed for ease of use for any lab experiment with gel imaging. SmartView Pro Imager System is packed with 5.0 megapixels high sensitivity and resolution camera for outstanding imaging quality.
1.3 Components guide Front View Camera and lens Viewing window Darkroom door with UV safety switch LED indicator light A-type USB port Main power Power socket & Fuse holder Rear View Side View...
Section 2 Technical Specification Model Name UVCI-1100 Total Weight Approx. 24.5 kg Unit Dimension 410 × 405 × 570mm (W × D × H) Construction Material Painted iron metal, ABS front door Display LED indicator light Rated Voltage 240V~, 50/60Hz, 2A Power 120W Connectivity...
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(optional) White Light Table Viewing Window Built-in UV viewing window Built-in viewing window Amber filter (560nm) PC Minimum Hardware Requirements 1.8GHz Pentium® IV or equivalent AMD Athlon® Processor processor Memory Storage 1GB available HD space Media CD-ROM drive Connectivity 1 port USB 2.0 Display 1280 ×...
Section 3 Installation Instructions SmartView Pro Imager System comes with epi white light and trans-UV as standard. Optional blue light module or white light table is offered for expansion to offer higher flexibility. There are few procedures to install the optional devices: place the unit on a sturdy, level safe and dry place, then follow the instruction below for installation.
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Step2 Open the darkroom door and hold the left blue light on left inner wall. Step3 Connect the blue light quick connector with darkroom inner socket.
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Step4 Insert the blue light into the inner lining of darkroom. Step5 Put the other side of blue light into the stud of darkroom inner wall.
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Step6 Take a nut from parts kit. Use 7M/M socket wrench to tighten the nut on the stud. Step7 The blue light is fixed as shown below. Please repeat steps 2~6 for right side blue light to install it in the darkroom.
3.2 Installing White Light Table Module (Optional) Step1 Get the white light table module ready. There are one white light table and 2 pieces of M7 nuts in the package. Before assembling, please check to make sure the screw studs on both sides of the white light table are in the upright position.
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Step3 Connect the white light table quick connector with darkroom inner socket Step4 Make sure the stud holders on the white light table align with the studs of darkroom.
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Step5 To take nuts from parts kit. Use 7M/M socket wrench to tighten nuts on the studs. Right Left Step6 The white light table is fixed as shown below. Left Right...
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Step7 All accessories are installed as shown below. Note: If necessary, please manually adjust the aperture to have the best performance while working with White Light Table. Aperture ring F1.2 - Close For more details, please refer to the operation instructions on 4.2.6.
3.3 Install SmartView Pro 1100 software Step1 Select UVCI-1100 setup program. According to user s system, the software will choose language automatically. (English/Traditional Chinese/Simplified Chinese) Step2 Install SmartView Pro 1100 setup program.
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Step3 Accept the license agreement and then press the next to proceed. Step4 Save this program in your specifying location then press Next to proceed.
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Step5 Click install to begin the installation. Step6 Press "Finish" to complete the program installation.
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Note: Please restart the computer when you install all software well down. Step8 The short paths will be shown on desktop. Step9 Double click the SmartView Pro 1100 Imager System icon in order to key in the license key. (on the camera and CD box cover)
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License Key Note: The license key must match the camera serial number. Note: Do not unplug the USB wire of SmartView Pro Imager System when you operate the analysis software. If you need to disconnect the SmartView Pro Imager System with your computer, please close the analysis software first.
Section 4 Operation Instructions 4.1 Capture/Analysis Control interface (23) (3)~(18) Function (19)~(22) Function...
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Display screen: This area shows the real-time or captured image. Camera Status : Connect Camera Status : Disconnect Function: Capture: This button allows you to acquire the current image and save it Real-time Crop: Crop the image you need and press Capture to save the area you just cropped.
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Analysis: Process to the gel analysis software. Blue Light: ON/OFF switch for the Blue light. White Light: ON/OFF switch for the White light. White Light Table: ON/OFF switch for the White light table. UV: ON/OFF switch for the UV transilluminator UV Intensity: Change the UV light intensity between 70% and 100% illumination.
Auto Exposure: Adjust the exposure time of camera automatically. Press Auto Exposure disable the function first if you want to manually adjust the exposure time. Note: Depending on the autoexposure setting, the system may take up to 30 seconds for the camera to process and calculate the best exposure time.
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(2)~(13) a. Open the data file. b. Save the file. c. Exit the program. Step: Next Step: Go to next step to select analysis mode: gel positive/ gel negative/ dot positive/ dot negative. Process: Crop: Select the cropping area of the image.
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Rotate: Rotate the image. Flip: Flip the image according to the orientation you desired. Brightness: Adjust the brightness of the image. Contrast: Adjust the contrast level of image. Negative: To reverse the image between black and white (not applicable to colored images). Zoom: To show a larger area or to examine more closely of the image.
(1)~(4) Step: Previous Step: Return to previous step, current setting will be deleted. Next Step: Go to next step. Editor: Add Lane: Add the lane to analysis. Delete Lane: To cancel the lane.
Note: Be sure to add all lanes you wish to analyze before you proceed to the next step. (1)~(9) Step: Previous Step: Return to previous step, current setting will be deleted. Next Step: Go to next step.
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Home: Return to Image Process Interface. Analyze: Auto Find: Find the peaks automatically. Add Peak: Add the peak manually. Delete Peak: Delete the peak manually. Base Line: Set the base line manually for selecting peaks. Previous Lane: Go back to last lane. Next Lane: Go to next lane.
4.2 Start the Operation To have a better result when documenting with the blue light as lighting source, the recommended stain selection guide table is provided below, choose the appropriate dye to have the gel stained: Experimental Protocol Nucleic Acid Stain Performance Post Sample...
Place the gel onto the center of the UV Transilluminator. Make sure you use the appropriate filter, the default type is optical EtBr filter. For different applications, please contact Major Science or your regional distributor for suitable filters. Note: For use with UV light as activation source, optical filters should be used.
Turn on the SmartView Pro Imager System Connect the SmartView Pro Imager System with the computer by USB wire. With the system turned on, double click the SmartView Pro system icon in order to key in the license key. License Key Note: The license key must match the camera serial number.
For adjusting exposure time, refer to section 4.1.1: Time set. Note: (1) Depending on the autoexposure setting, the system may take up to 30 seconds for the camera to process and calculate the best exposure time. The autoexposure time is capped at a maximum of 3 seconds to prevent the system from over-exposure.
Nucleic acid samples imaging (Agarose gel) You may use UV light for Nucleic acid DNA sample imaging. You can switch between UV high (100%) and UV low (70%) to get better signals. With environmental safe dye (such as Novel Juice and Midori Green Direct), you may use optional blue light module for less invasion to your DNA sample Protein samples imaging (Protein gels such as Coomassie blue or...
The system allows the user to change the default settings by pressing the button (as box1). After setting up the parameters, "Start up settings" (as box 2) will come into effect once you restart the system and act as default permanently until you change it next time.
Advanced features: The display will show the Saturation The display will show the date stamp if you warning if you enable the saturation enable the Date stamp from Advanced detection from Advanced features. features. The image will adjust to saturation detect level you set.
To capture the image, click on the Capture button then a dialog will pop up to save the image. Select the image file format you desire and choose the data location and give a file name. To capture an image with the real-time crop function, click on the "Real-time Crop"...
4.3 Image Analysis: Using Gel Positive/Gel Negative to analyze the sample To start the image analysis, first you will have to choose your image from the data file. Step1 Press Analysis (as box 1) to select your image. You can download the image file from the hard drive or the USB drive.
Step2 The image will be shown on the center of a new window. You will be directed to the image processing tool box as shown below. Step1 Crop: Crop the image by press the Crop button (as box 1). In order to select the cropping area of the image, hold and drag the cursor to select the area you want (as box 2).
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Step1-1 The image will be shown below as you selected. * Remember: Always rotate the image before you crop!
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Step2 Rotate: To rotate the image, first select the Rotate button (as box 1) then the toolbar (as box 2) will pop up. Toolbar: : Rotate counter-clock wise : Rotate clock wise : Confirm action : Cancel...
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Step2-1 You may rotate the image counter-clock wise or clock wise in two ways: a. Using the toolbar labelled as box 1, press " " to confirm action. b. Input the desired angle (0~359) directly in the blank space labelled as box 2, press ENTER first before pressing "...
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: Confirm action : Cancel Step4 Brightness: Adjusting the brightness of the image by pressing on the Brightness button (as box 1), then use the scrollbar (as box 2) to adjust the brightness level. : Low High Step4-1 The result of Brightness is shown below. Press to proceed or press to cancel.
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Step5 Contrast: Adjusting the contrast level of the image by pressing the Contrast button (as box 1), then use the scrollbar (as box 2) to adjust the contrast level. : Low High...
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Step5-1 The result of Contrast is shown below. Press to proceed or press to cancel. : Confirm action : Cancel Step6 Negative: To reverse the image between black and white by pressing on the Negative button (as box 1). If you don t want to negative the image, you can press the Negative button again or you can press the Undo button (as box 2) to recover the image.
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Step7 Zoom: Adjusting the zoom of the image by pressing on the Zoom button (as box 1) and then a toolbar will pop out. If you want to zoom in the image, press the + button (as box 2). If you want to zoom out the image, press the - button (as box 2).
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Step8 Text: You could add any text onto the image. Click on the Text button (as box 1) and then the toolbar (as box 2) will show up. The sample text (as box 3) will show on middle of the screen. Step8-1 Click on the input box, type in your text here.
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: Confirm action : Cancel Note: 1. To add another set of text, please press "TEXT" again. ndo .
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Step9 Original: This is the master undo button. It will undo ALL the changes you made to the image and revert it back to the original image. A massage will pop up to ask for confirmation once you press the Original button.
Step1 Add Lane: Selecting your lane by creating a long rectangular box along the lane. Press Add Lane button (as box 1) and then hold and drag the rectangular box to the area you want. Each time you press Add Lane button, the system will duplicate an exact box as your previous box.
The analysis software will analyze the bands inside the lane to create a histogram.
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Step1 Auto Find: Click on the Auto Find button (as box 1), the software will allow you to adjust the sensitivity level according to your needs. Sensitivity level (as box 2) is ranged from 1 to 10. Select a number appropriate to your image.
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Example B: Sensitivity level 10 Step1-2 If the density of band (as box 1) is not clear, the Auto Find (as box 2) function cannot find any peaks.
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Step1-3 The warning dialog will pop up on the screen. Step2 Add Peak: Click Add Peak button (as box 1), choose two points which covers the range of the peak from the screen (select one point from the left side and a second point from the right side of the desired peak) to add a peak (as box 2) and then click the...
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Step2-1 The peak will turn RED . The new peak number is 6 (shown below). Step3 Delete Peak: Press Delete Peak button (as box1), click on that peak (as box 2), the peak will turn gray and then click to finished.
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Step3-1 The peak will turn white. The white peak will not be included in the calculation. The order of the peaks will be rearranged. Step4 Base Line: This feature is used to help you increase (or cut off) the peaks you want (or do not want) to include in the quantification step. Press Base Line button (as box 1) a green line will show up on the screen, you could move the line to the position you desiredand the peaks above the base line will be selected, then press...
Note: You need to have at least one peak in order for Base Line feature to function. Step4-1 The software will only count the peaks that are above the base line. There were three lanes selected (as box 1) in this example, so you need to press on the Next Lane button (as box 2) go to select peaks for lane3 before proceeding to summarizing the data.
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Step2 Double click the blank L1 Concentration box associated with the known marker size. After key in the first value, the system will show a dialog to remind you to key in the second value. Note: The concentration values of marker are provided by marker manufactuerer.
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Step3 Entering the second value of known concentation then hit Enter on your keyboard to finish, the analysis software will project all the other unknown concentrations based on the 2 known (input) concentrations. Step4 Click Save image button (as box 1) to store this image from the screen.
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Step5 Click on the Export Data button (as box1), a window pops up to ask you to select a destination location to save your file. Give the data a file name (as box 2) and press Save (as box 3). (Data is then exported using the CSV file format, which could be easily access by using Microsoft Excel) 4.3.5.2 Calculating the molecular weight...
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Step2 Key in the respective molecular weight values of the marker in this page. Note: The Mol weight values of marker are provided by marker manufacturer. Click on the Mol weight box and then input all known values. Step3 Fill in all known values of the marker on the list.
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Step4 Click Save image button (as box 1) to store this image from the screen. Choose a destination location to save your image. Give the image a file name (as box 1) and press Save (as box 2) to finish. Step5 When clicking the Mol Weight Calc.
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Step6 Choose a destination location to save your export. Give the data a file name (as box1) and press Save (as box 2) to finish.
4.4 Image Analysis: Using Dot blot positive/ Dot blot negative to analyze the sample Step1 Press Analysis (as box 1) to select the image you wish to analyze. You may select the image file from the hard drive or the USB drive. Select an image from the folder (as box 2).
You will be directed to the image processing tool box as shown below. Step1 Crop: Crop the image by press the Crop button (as box 1). In order to select the cropping area of the image, hold and drag the cursor to select the area you want (as box 2).
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Step2 Rotate: To rotate the image, first select the Rotate button (as box1) and the toolbar (as box 2) will pop up. Toolbar: : Rotate counter-clock wise : Rotate clock wise : Confirm action : Cancel...
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Step2-1 You may rotate the image counter-clock wise or clock wise in two ways: a. Using the toolbar labelled as box 1, press " " to confirm action. b. Input the desired angle (0~359) directly in the blank space labelled as box 2, press ENTER first before pressing "...
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: Invert the image from left to right. : Confirm action : Cancel Step4 Brightness: Adjusting the brightness of the image by pressing on the Brightness button (as box 1), then use the scrollbar (as box 2) to adjust the brightness level. : Low High...
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Step4-1 The result of Brightness is shown below. Press to proceed or press to cancel. : Confirm action : Cancel...
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Step5 Contrast: Adjusting the contrast level of the image by pressing the Contrast button (as box 1), then use the scrollbar (as box 2) to adjust the contrast level. : Low High Step5-1 The result of Contrast is shown below. Press to proceed or press x to cancel.
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Step6 Negative: To reverse the image between black and white by pressing on the Negative button (as box 1). If you don t want to negative the image, you can press the Negative button again or you can press the Undo button (as box 2) to recover the image. Step7 Zoom: Adjusting the zoom of the image by pressing on the Zoom button (as box 1) and then a toolbar will pop out.
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Step8 Text: You could add text onto the image. Click on the Text button (as box 1) and then the toolbar (as box 2) will show up. The sample text (as box 3) will show on middle of the screen.
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Step8-1 Click on the input box, type in your text here. You could adjust your text using this toolbar. The toolbar functions are explained as below. : Change the font type : Change the font size. : Move the text to the desired location by move the mouse cursor to the desired location on the image and click the left button of the mouse.
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Step9 Original: This is the master undo button. It will undo ALL the changes you made to the image and revert it back to the original image. A massage will pop up to ask for confirmation once you press the Original button.
Press blot Positive button and the software will automatically analyze the dot-blot samples from the image. Step1 Add Dot: Click Add Dot button (as box 1) to add one sample. Use the mouse to select the sample (as box 2) from the screen. Each time you press the Add Dot button, the system will duplicate a random circle size.
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Step1-1 The image will be shown below as you have selected. Step2 Delete Dot: Press Del Dot button (as box 1), and then select the dot (as box 2) you want to delete. After selecting the dot, click on Next Step button (as box 3) to move forward to next step.
Step1 Double click on the Dot concentration blank boxes to input the real density of each sample. (You will need at least one value in order to calculate the rest of the numbers.) Step2 Enter the value of known concentration. The analysis software will project other unknown concentrations based on the known (input) concentration.
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Step3 Click Save image button (as box 1) to store this image from the screen. Choose a destination location to save your image. Give the image a file name (as box 2) and press Save (as box 3) to finish. Step4 Click on the Export Data button (as box 1), a window pops up to ask you to select a destination location to save your file.
Section 5 Troubleshooting Guide Many operating problems may be solved by carefully reading and following the instructions in this manual accordingly. Some suggestions for troubleshooting are given below. Should these suggestions not resolve the problem, please contact our SERVICE DEPARTMENT or a distributor in your region for assistance.
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The software displays Please contact the service department of Major Science or your local distributor. You did not connect the computer with device. Check the connection between the computer and device. You did not install the drivers on the computer. Check the detail instruction of installation in Section 3.3.
Always disconnect the SmartView Pro Imager System from the electrical power prior to cleaning. 6.1 Replacing the Fuse For additional fuses, contact Major Science Co., Ltd. To replace the fuse: 1. Turn off the main power switch at the rear of system and detach the power cord.
6.2 Adjust the camera for clearer image Tool: 2.5 mm hex wrench (not provided) Step1 Open camera door. Remove the filter to a stable place.
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Step2 Use 2.5mm hex wrench to loosen the screws of camera holder and hold the camera to prevent damage to the lens. Step3 Pull out the camera gently.
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Step4 Disconnect the USB port with camera. Step5 Separate the camera with darkroom, you could use general camera cleaning kit to clean the lens and then reinstall the camera on the camera holder for clearer image.
6.3 Replacing Amber Filter onto Viewing Window Two filters (transparent filter and amber filter) are installed as default, if you did not purchase the blue light module and would like to remove the amber filter, please follow the steps below. Tool: 7M/M socket wrench (not provided)
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Step1 Loosen 4 nuts from the corners. Remove the transparent filter. Step2 There are no screw holes on the amber filter, please put the amber filter on the inside of the bolt.
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Step3 Cover the transparent filter on the amber filter, put the nuts of four corners and then use the socket wrench to tighten the nuts. Step4 Confirm the amber filter is be fixed in the center of the viewing window.
6.4 Adjust the scientific camera when out of focus Adjust the scientific camera for out of focus only under SmartView Pro 1200 Imager System pc version to operate, please refer to SmartView Pro 1200 Imager System pc version instruction manual to install the software on your computer.
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Step2 Put fluorescent ruler on the UV table again. Turn on the UV light and press Size button (as box 1) to make sure the picture is clear and focus on. Fix the focus ring after adjust.
ODELS PPLICATIONS Cat. No. Description UVCI-1100 SmartView Pro 1100 Imager system, standard version SmartView Pro 1100 Imager system, standard version, high UVCI-1101 UV performance Filter (for camera) Ordered Separately UVCI-1000-EB Optical EtBr Filter 610nm for SmartView Pro & Simple Imager System UVCI-1000-SG Optical SYBR Green Filter 520nm for SmartView Pro &...
Appendix: Install Camera Software * To install the program, please log in as an administrator on the computer. Please refer to the Web link to change the account of computer: a. For Windows® XP http://www.microsoft.com/resources/documentation/windows/xp/all/proddocs/en-us/windows_ security_runas.mspx?mfr=true b. For Windows® 7 http://windows.microsoft.com/en-hk/windows7/installing-programs-frequently-asked-questions For Windows®...
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Step2 Check computer system type. Step3 Install camera setup software. Note: Five files are included in the software disk. Please accord to your system type to install camera setup software. System type A. 32 bit : StCamSWare_x86 (as box 1) System type B.
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Step4 Select the language for your location. Step5 Step6...
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Step7 Step8 Save this program in destination folder.
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Step9 Click install to begin the installation. Step10 Complete the installation program.
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Note: Under Windows XP system, if you want to change the USB port from computer/laptop with camera, a found new hardware dialog of the system will pop up. Please install the hardware as following to proceed. 1. Select No, not this time then press Next button. 2.
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3. Press Continue Anyway to finish the hardware wizard.