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3
Storage conditions and preparation of working
solutions
Attention: Buffers RCU contains chaotropic salt. Wear gloves and goggles!
CAUTION: Buffer RCU contains guanidinium thiocyanate which can form highly reactive
compounds when combined with bleach (sodium hypochlorite). DO NOT add bleach or acidic
solutions directly to the sample-preparation waste.
•
All kit components should be stored at room temperature (18–25 °C) and are stable up
to one year. Storage at lower temperatures may cause precipitation of salts.
•
Check that 96–100 % ethanol is available as additional solution in the lab.
Before starting any NucleoSpin
•
Clean-up Buffer RCU: Add the indicated volume of 96–100 % ethanol to the Clean-up
Buffer RCU Concentrate. See table below or bottle label for necessary volumes. Store
Buffer RCU at room temperature (18–25 °C) for up to one year.
•
Wash Buffer RA3: Add the indicated volume of 96–100 % ethanol (see table below) to
Wash Buffer RA3 Concentrate. Mark the label of the bottle to indicate that ethanol was
added. Store Wash Buffer RA3 at room temperature (18–25 °C) for up to one year.
REF
Clean-up Buffer
RCU (Concentrate)
Wash Buffer RA3
(Concentrate)
RNA clean up XS
®
RNA Clean-up protocol, prepare the following:
NucleoSpin
10 preps
740903.10
5 mL
Add 15 mL ethanol
6 mL
Add 24 mL ethanol
MACHEREY-NAGEL – 03/2021, Rev. 05
®
RNA Clean-up
50 preps
740903.50
5 mL
Add 15 mL ethanol
12 mL
Add 48 mL ethanol
250 preps
740903.250
5 x 5 mL
Add 15 mL ethanol to
each bottle
50 mL
Add 200 mL ethanol
9
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