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Summary of Contents for Macherey-Nagel NucleoSpin RNA Clean-up XS
- Page 1 MACHEREY-NAGEL User manual RNA clean-up n NucleoSpin RNA Clean-up XS ® March 2021 / Rev. 05 www.mn-net.com www.mn-net.com...
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Page 2: Table Of Contents
5 Protocols 5.1 RNA clean up and concentration of RNA 5.2 DNA digestion in crude RNA extracts and subsequent clean up 6 Appendix 6.1 Troubleshooting 6.2 Ordering information 6.3 Literature 6.4 Product use restriction / warranty MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 3: Components
NucleoSpin RNA Clean-up XS Binding Columns (light blue rings – plus Collection Tubes) Collection Tubes (2 mL) Collection Tubes (1.5 mL) User manual * For preparation of working solutions and storage conditions see section 3. MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 4: Reagents, Consumables, And Equipment To Be Supplied By User
All technical literature is available on the internet at www.mn-net.com. Please contact Technical Service regarding information about changes of the current user manual compared to previous revisions. MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 5: Product Description
(e.g., RT-PCR). RNA enrichment of 20 x up to 50 x can be achieved (e.g., input: 300 µL sample containing crude RNA (10 ng/µL); output: 5 µL eluate containing pure RNA (510 ng/µL); enrichment of factor 51 (MACHEREY-NAGEL in-house data)). -
Page 6: Handling, Preparation, And Storage Of Starting Materials
RNA sample. The stability of prepurified RNA samples (e.g., RNA isolated with phenol based protocols) depends very much on the performed procedure. Wear gloves at all times during the preparation. Change gloves frequently. MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 7: Elution Procedures
Eluted RNA should immediately be put and always kept on ice during work for optimal stability! Contamination with almost omnipresent RNases (general lab ware, fingerprints, dust) may be a risk for isolated RNA. For short-term storage freeze at -20 °C, for long-term storage freeze at -70 °C. MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 8: Storage Conditions And Preparation Of Working Solutions
Add 15 mL ethanol Add 15 mL ethanol Add 15 mL ethanol to each bottle Wash Buffer RA3 6 mL 12 mL 50 mL (Concentrate) Add 24 mL ethanol Add 48 mL ethanol Add 200 mL ethanol MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 9: Safety Instructions
* Hazard labeling not neccessary if quantity per bottle below 125 g or ml (certificate of exemption according to 67 / 548/EEC Art. 25, 1999 / 45/EC Art. 12 and German GefStoffV § 20 (3) and TRGS 200 7.1). For further information see Material Safety Data Sheet. MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 10: Protocols
For high demanding applications, the recovery rate can further be increased as follows: Centrifuge 30 s at 2,000 x g prior to centrifugation for 30 s at 11,000 x g. MACHEREY-NAGEL – 03/2021, Rev. 05... - Page 11 If higher RNA concentrations or higher elution volumes are desired, elution volume may be varied in the range of 5–30 µL. 11,000 x g, 30 s For further details on alternative elution procedures see section 2.4. MACHEREY-NAGEL – 03/2021, Rev. 05...
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Page 12: Dna Digestion In Crude Rna Extracts And Subsequent Clean Up
RNase control and subsequent repurification of the RNA (in order to remove buffer, salts, DNase, and digested DNA) are usually required. The MACHEREY-NAGEL rDNase Set (to be ordered separately, see ordering information), contains high quality, recombinant RNase-free DNase (rDNase) and reaction buffer. It is optimized for a highly efficient digestion in order to remove even traces of contaminating DNA. -
Page 13: Appendix
Sample material not stored properly. Keep thawed samples on ice before addition of Buffer RCU. Sample material already contaminated with DNA Contamination of RNA with • Digest contaminating DNA in an RNA sample according to section genomic DNA 5.2. MACHEREY-NAGEL – 03/2021, Rev. 05... - Page 14 Unexpected the initially measured A and A values are significantly above ratio the detection limit of the photometer used. An A value close to the background noise of the photometer will cause unexpected ratios. MACHEREY-NAGEL – 03/2021, Rev. 05...
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Page 15: Ordering Information
FFPE RNA 740969.10 10 preps 740969.50 50 preps 740969.250 250 preps ® NucleoSpin RNA/DNA Buffer Set 740944 Suitable for 100 preps rDNase Set 740963 1 set ® NucleoSpin Filters 740606 Collection Tubes (2 mL) 740600 1000 MACHEREY-NAGEL – 03/2021, Rev. 05... -
Page 16: Literature
DNA/RNA/PROTEIN purification products of MACHEREY-NAGEL are suitable for IN-VITRO- USES ONLY! ONLY MACHEREY-NAGEL products specially labeled as IVD are also suitable for IN-VITRO- diagnostic use. Please pay attention to the package of the product. IN-VITRO-diagnostic products are expressly marked as IVD on the packaging. - Page 17 Supplementary reference is made to the general business terms and conditions of MACHEREY-NAGEL, which are printed on the price list. Please contact us if you wish to get an extra copy.
- Page 18 Tel.: +49 24 21 969–270 tech-bio@mn-net.com Trademarks: LightCycler™ is a trademark of a member of the Roche Group ® NucleoSpin is a registered trademark of MACHEREY-NAGEL GmbH & Co KG ® RiboGreen is a registered trademark of Thermo Fisher Scientific ® SYBR is a registered trademark of Molecular Probes, Inc.
- Page 19 MACHEREY-NAGEL GmbH & Co. KG Tel.: +49 24 21 969-0 info@mn-net.com Neumann-Neander-Str. 6–8 Tel.: +41 62 388 55 00 sales-ch@mn-net.com 52355 Düren · Germany Tel.: +33 388 68 22 68 sales-fr@mn-net.com A035557/0310.3 Tel.: +1 484 821 0984 sales-us@mn-net.com...
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