1.2
Reagents, consumables, and equipment to be supplied
by user
Reagents
•
96–100 % ethanol (to prepare Wash Buffer RA3 and prepare Clean-up Buffer RCU and
Wash Buffer RA3)
Consumables
•
1.5 mL microcentrifuge tubes
•
Sterile RNase-free tips
Equipment
•
Manual pipettors
•
Vortex mixer
•
Centrifuge for microcentrifuge tubes
•
Personal protection equipment (e.g., lab coat, gloves, goggles)
1.3
About this user manual
It is strongly recommended reading the detailed protocol sections of this user manual if the
®
NucleoSpin
RNA Clean-up XS kit is used for the first time. Experienced users, however,
may refer to the Protocol at a glance instead. The Protocol at a glance is designed to be used
only as a supplemental tool for quick referencing while performing the purification procedure.
All technical literature is available on the internet at www.mn-net.com.
Please contact Technical Service regarding information about changes of the current user
manual compared to previous revisions.
RNA clean up XS
MACHEREY-NAGEL – 03/2021, Rev. 05
5