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3
Storage conditions and preparation of working
solutions
Attention: Buffer A3 contains guanidine hydrochloride! Wear gloves and goggles!
CAUTION: Buffer A3 contains guanidine hydrochloride which can form highly reactive
compounds when combined with bleach (sodium hypochlorite). DO NOT add bleach or
acidic solutions directly to the sample-preparation waste.
• All kit components can be stored at room temperature (18–25 °C) and are
stable for at least one year.
• Always keep buffer bottles tightly closed, especially if buffers are preheated
during the preparation.
• Storage of Buffer A2 below 20 °C may cause precipitation of SDS. If salt
precipitate is observed, incubate buffer at 30–40 °C for several minutes and
mix well until all precipitate is redissolved completely. Cool down to room
temperature before use.
Before starting any NucleoSpin
• Add Liquid RNase A to Buffer A1 and mix thoroughly. Indicate date of RNase A
addition. Store Buffer A1 containing RNase A at 4 ° C. The solution will be stable
at this temperature for at least six months.
• Add the indicated volume of 96–100 % ethanol to Buffer AQ (Concentrate).
REF
Wash Buffer AQ
(Concentrate)
10
Plasmid DNA purification
Plasmid EasyPure protocol prepare the following:
®
NucleoSpin
10 preps
740727.10
6 mL
Add 24 mL ethanol
MACHEREY-NAGEL – 08 / 2 013, Rev. 01
Plasmid EasyPure
®
50 preps
740727.50
6 mL
Add 24 mL ethanol
250 preps
740727.250
30 mL
Add 120 mL ethanol
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