Handling, Preparation, And Storage Of Starting Materials - Macherey-Nagel NucleoSpin RNA Clean-up XS User Manual

Table 1: Kit specifications at a glance
Parameter
Technology
Format
Sample material
Fragment size
Typical recovery
A /A
260 280
Elution volume
Preparation time
Binding capacity
2.3

Handling, preparation, and storage of starting materials

RNA intended to be used as sample for the NucleoSpin
should be handled with the same care as any RNA sample. The stability of prepurified
RNA samples (e.g., RNA isolated with phenol based protocols) depends very much on the
performed procedure.
Wear gloves at all times during the preparation. Change gloves frequently.
RNA clean up XS
®
NucleoSpin RNA Clean-up XS
Silica membrane technology
Mini spin columns – XS design
< 300 µL RNA solution containing < 90 µg RNA
> 200 nt
85–95 %
1.9–2.1
5–30 µL
Approx. 20 min/6 preps
110 µg
MACHEREY-NAGEL – 03/2021, Rev. 05
®
RNA Clean-up XS procedure
7