Elution Procedures; Stability Of Isolated Rna - Macherey-Nagel NucleoSpin RNA Clean-up XS User Manual

2.4

Elution procedures

A high RNA concentration in the elution fraction is desirable for all typical downstream
applications. In particular with regard to limited volumes of reaction mixes, high RNA
concentration can be a crucial criterion. Due to a high default elution volume, standard kits
often result in low concentrated RNA, if only small samples are processed.
Such RNA often even requires a subsequent concentration to be suitable for the desired
application.
In contrast to standard kits, NucleoSpin
very small volume resulting in highly concentrated RNA.
Elution volumes in the range of 5–30 µL are recommended, the default volume is 10 µL.
2.5

Stability of isolated RNA

Eluted RNA should immediately be put and always kept on ice during work for optimal
stability! Contamination with almost omnipresent RNases (general lab ware, fingerprints,
dust) may be a risk for isolated RNA. For short-term storage freeze at -20 °C, for long-term
storage freeze at -70 °C.
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RNA clean up XS
®
RNA Clean-up XS allows an efficient elution in a
MACHEREY-NAGEL – 03/2021, Rev. 05