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Using the Column Oven
Shutting Down the System
When shutting down the system overnight or for the weekend, observe all
precautions pertaining to hazardous solvents and/or those solvents which
form harmful deposits or by-products.
1. Remove harmful mobile phases from the LC system.
2. Flush the column according to the instructions supplied with the column.
Flush buffer salts from the system with water; evaporation leaves salt
crystals which may form harmful deposits. Remove chloroform or
solvents which can decompose to form hydrochloric acid from the system.
3. After removing harmful mobile phases, prepare the detector for most
mobile phases by flushing it with isopropanol.
To avoid contaminating the system, re-filter or discard solvents
(including water) that were exposed to the environment for more
than 24 hours before use.
4. For weekend storage we recommend flushing 60%/40% MeOH/Water
through the pump, column, and flowcell (provided your column is
compatible with MeOH/Water). Then turn off the column oven, pump,
and detector.
Damage caused to the system by precipitating buffer
salts in capillary tubing, or damage resulting from this
condition, is specifically excluded from warranty.
Shutting Down the System
3-3
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