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1-8 Introduction
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Carefully use and store flammable solvents, which may form hazardous
by-products when the instrument is shut down, by following the
recommended shutdown procedure.
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Check compatibility of solvent(s) with the type of column(s) being used.
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Know the relative polarity and miscibility of the solvents being used.
Buffers
Exercise care when using buffers in conjunction with organic solvents.
NEVER LEAVE BUFFERS IN THE SYSTEM OVERNIGHT. Buffers left in
the system can form salt crystals, which may cause premature pump seal
failure, interfere with proper check valve operation, and plug the connecting
tubing or the detector flowcell.
To remove buffers, flush the system first with water, then with methanol
or isopropanol. Change the mobile phase from methanol or isopropanol to
water before using buffers.
Corrosion
All parts that contact the mobile phase are made of stainless steel, Teflon,
PEEK, or glass. Some of these materials are extremely sensitive to acid
chlorides. If you have questions about your mobile phase, contact a
PerkinElmer representative.
Table 1-1. Solvents Which May Corrode the Series 200 Peltier Column Oven
Aqua Regia (80% HCl, 20% HNO 3 )
Chlorinated solvents
Sulfuric Acid (Conc.)
*
Titanium is more resistant to chloride salt solutions.
Air Bubbles
To prevent air from entering the system, and to ensure that pressure
fluctuations do not occur, observe the following precautions:
Ensure that the pump's solvent inlet filter is below the solvent level in
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the solvent reservoir.
If the pump has not been used for an extended period of time, remove air
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bubbles by connecting a priming syringe to the drain valve on the pump,
opening the drain valve, and fast-flushing the system to prime the pump.
After priming the pump, close the drain valve completely.
Warnings and Precautions
Chloride salt solutions*
Halogenated solvents
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