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JEOL JEM1400 Operation page 9

Transmission electron microscope

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 Set magnification 80,000-100,000x using MAG/CAML knob (right console)
 Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It
may help condense the beam before doing this (BRIGHTNESS knob).
 use AMT camera capture engine program
 Focus the sample. Use FOCUS coarse and fine knobs (right console).
 Select 'FFT' in AMT camera imaging modes list. New 'FFT' window opens – black
background with white shape in the centre.
 Use FOCUS coarse. Go through focus forward and backward and try to maximise size
of the white shape, so its form become more visible
 The white shape should be circular, if not need to be adjusted.
 To adjust, press 'OBJ STIG' button (left console)
 Use the DEF/STIG knobs, X and Y, to get white shape circular form.
 Press 'BRIGHT TILT' button to return to imaging mode.
 Cancel 'BRIGHT TILT' button
Reduce magnification to 4,000x using MAG/CAML knob (right console).
Use BRIGHTNESS knob to reduce illumination of the beam.
EM Suite 15/11/2016
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