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Alignments
1. Use fluorescent screen and, if needed, binoculars with small fluorescent screen
2. Center condenser aperture
Set magnification 5,000x using MAG/CAML knob (right console)
Condense beam to a spot with 'BRIGHTNESS' knob
Move beam to center of fluorescent screen with Shift ('X', 'Y') knobs
Expand beam with 'BRIGHTNESS' knob
When centered, beam will expand symmetrically around the fluorescent screen
Accurately adjust X and Y knobs (do not move the knobs more than 1/4 turn) on
condenser aperture until expansion is symmetrical
3. Voltage center adjustment
Find your sample by using trackball or pressing arrows on sample control console.
Find a feature to align and focus your sample on – e.g. unevenness in the film, edge
of a resin section etc
Set magnification 30,000x using MAG/CAML knob (right console)
Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It
may help condense the beam before doing this (BRIGHTNESS knob).
Press HT wobbler button (right console) – the beam will start consistently
condensing-spreading.
Use binoculars and small fluorescent screen. Adjust brightness (BRIGHTNESS knob)
to comfortably see the feature. The image should pulse on center.
If not, press Bright Tilt button (left console) and use DEF/STIG knobs (X and Y, on left
and right consoles) to align.
Turn off Bright Tilt and HT wobbler buttons.
Hint: do one knob at a time and minimize movement.
Note. If the image shifts significantly while focusing, check Voltage Center again.
4. Adjust gun shift
a) Set magnification 15,000x using MAG/CAML knob (right console)
b) Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It
may help condense the beam before doing this (BRIGHTNESS knob).
c) Select spot-size – 5 ('SPOT SIZE' knob, left console, to confirm and check use 'TEM
System Task Bar' window)
d) Condense the beam to smallest size
e) If beam position will be not in the centre of the screen, press 'BRIGHT TILT' button
(left console) and use the SHIFT knobs, X and Y, to move the beam back to the
centre.
f) Cancel 'BRIGHT TILT' button
g) Select spot-size – 1 ('SPOT SIZE' knob, left console, to confirm and check use 'TEM
System Task Bar' window)
EM Suite 15/11/2016
P a g e
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