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Press 'Standard Focus' button on right console to neutralize the focus
Press Wobbler button X or Y ('IMG WOB') on right console.
Use the '+Z' and '−Z' buttons on right console (or '+Z' and '−Z' buttons in the 'Stage
Controller' window) to move stage z-axis and bring image into fine focus.
Adjust focus until the image wobble is minimized. You will be at or near the focus
point.
Turn off the wobbler
9. Align microscope (see page 7-9, alignment instruction). Skip if used for basic imaging.
10. Focusing:
Use FOCUS coarse and fine knobs (right console).
Use Wobbler button X or Y ('IMG WOB') on right console. Adjust focus until the
image wobble is minimized. You will be at or near the focus point. The image
wobbler is useful for rough focusing at magnifications up to ～50,000×.
Turn off the wobbler when focus is established and before recording an image
11. Record images (see instructions below)
12. LOW MAGNIFICATION imaging
Switch from normal magnification mode
Choose magnification of 800x using MAG/CAML knob (right console)
Press 'LOW MAG' button (right console).
Remove objective aperture – rotate big wheel and place red dot opposite black dot.
Switch back to normal magnification mode
Insert objective aperture in – rotate big wheel and place second big white dot
opposite black dot.
Press 'MAG2' button
Adjust objective aperture as described in step.7.
Get help if something is not right. Notify EM Suite staff if problems arise (e.g. vacuum in
13.
column gets worse, error message shows up, microscope shuts itself down).
EM Suite 15/11/2016
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