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Viewing and Imaging Specimen
1. Insert specimen (see page 5-6 of this instruction)
2. Use 'Valve/vacuum monitor' window. Make sure the specimen chamber says 'Evac
ready'.
3. Turn on filament. Use 'beam controller' window. Filament is on when 'On' and 'Beam
Ready' buttons turn bright green.
4. Record the start time in the log book.
5. Turn the lights off in the room, remove the cover of the viewing screen.
6. Check for beam on fluorescent screen. If no beam, then see instructions 'finding the
beam' (on separate sheet).
7. Insert objective aperture - rotate big wheel and place second big white dot opposite
black dot.
Set Magnification of 15,000x using MAG/CAML knob (right console)
Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It may
help condense the beam before doing this (BRIGHTNESS knob).
Using the BRIGHTNESS knob (left console), spread CW the beam until it reaches the
edges of the fluorescent screen
Press 'DIFF' button (right console). Microscope will be switched over to diffraction
mode. You should see a circle with bright spot in the centre. If bright spot is not
condensed use 'DIFF FOCUS' button (right console) to condense the bright spot (turn
and see which direction the spot gets condensed).
Centre the spot, if it is not centred. Press 'PLA' button (left console) and use
DEF/STIG knobs, X and Y.
Adjust X and Y knobs on objective aperture until bright spot become in the centre of
the circle.
Press 'MAG2' button (right console) to return to normal imaging mode.
Spread beam CW with BRIGHTNESS knob.
8. Set Z height. This can be done with stage binoculars + small fluorescent screen or with a
live camera image (see instruction below how to use camera).
Find your sample by using sample control console - rotate a trackball or press arrows
change the position of the sample.
Find a feature to align and focus your sample on – e.g. unevenness in the film, edge
of a resin section etc.
Choose magnification of 12,000x using MAG/CAML knob (right console)
Using the BRIGHTNESS knob (left console), establish a comfortable illumination for
imaging the sample.
Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It may
help condense the beam before doing this (BRIGHTNESS knob).
EM Suite 15/11/2016
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