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JEOL JEM1400 Operation page 2

Transmission electron microscope

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Hints or tips
1. 'BRIGHTNESS' knob (left console)
- CLOCKWISE (CW) – spread beam = decrease illumination
- ANTI-CLOCKWISE (ACW) – condense beam = increase illumination
2. CRS button –any controls are in the coarse mode (16-fold change) when the nearby is
illuminated
3. right console F1 button: 'Raise fluorescent screen'
4. right console F5 button: 'Beam Blank'
5. Double press 'LF2' button (left console): exchange sample – X, Y and Z neutralize and
filament is turned off.
6. In case of beam lost. Press 'LF3' button (left console). Reloads current saved alignment
settings for the microscope.
7. 'Nanospace Map' window: use it to move to specific areas on the stage and to memorize
different positions (select point, then click 'Shoot')
 Red point = current position
 Yellow square = memories
 Green square = current selected position
 Press Memory - position is stored. Text can be entered in the Memo column
 To delete a position – select position from the list and press 'Delete'
 To move stage to position needed – left click on the map where you want to be and
press 'Shoot'.
8. Do not change MAGNIFICATION when WOBBLER is on – microscope can glitch and
focusing become impossible. If that happened, close TEM Center and open it again
9. It is good practice to protect the chip of the camera from excess illumination using the
'Brightness MAG link' facility (See TEM center).
 Select a magnification of 25,000x
 Set the illumination using the camera
 Switch on 'Brightness MAG link' in the Illumination System window – the
illumination will now be maintained through the magnification range, up or down.
EM Suite 15/11/2016
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